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 MycoUnderground
Enthusiast
 
Registered: 10/23/08
Posts: 467
Loc: Suburban CO
Last seen: 12 years, 7 months
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 I think this is yeast...could use some confirmation 
#12094492 - 02/25/10 10:56 AM (14 years, 1 month ago) |
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These jars started great (MS). Most rhizomorphic growth I've had. I gave them a shake at about 50% colonization. After the shake they recovered, but did not seem as strong as before. As they were recovering I noticed small white specks in some parts of the mycellium. It is especially apparent when looking at the dark sunflower seeds that have a lot of contrast with the light mycellium and the specks.
I took pictures of the jar where the specks are most noticable. I took several to try to get the best focus (my camera is a pain).
As you can see there are spots around that sunflower seed and some less noticable ones around the rest of the jar. It looks to me like this is yeast or some type of bacteria.
What is most concerning me is other jars have similar specks in the mycellium, they are just much smaller; too small for my camera to show. I can't quite tell if it is mycellium or something else. Even though these jars are 100% colonized, will I be able to tell if they are contaminated by smelling when I open them? If not, is there any other way to tell if they are contaminated?
Thanks for checkin this out. I appreciate your responses.
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Hofmann1943
explorer
Registered: 03/05/09
Posts: 409
Loc: Forest
Last seen: 4 years, 18 days
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Re: I think this is yeast...could use some confirmation [Re: MycoUnderground]
#12102209 - 02/26/10 01:30 PM (14 years, 1 month ago) |
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if its yeast it will smell like one, and i don't think you'l have fully colonized jars, whit some bacteria like that in it.
open it smell it, if your still suspicious cut it out.
any way its look fine to me.
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By Albert Hofmann :
Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened.
That very small dosage, the first dose of my experiments i planed, was very very strong.
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Brennus
Student of Life
Registered: 05/31/08
Posts: 3,297
Loc: SE United States 
Last seen: 6 years, 1 month
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Re: I think this is yeast...could use some confirmation [Re: Hofmann1943]
#12102293 - 02/26/10 01:50 PM (14 years, 1 month ago) |
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Those jars look fine to me - but give them the smell test to be sure.
In my experience, grains infected with yeast won't get anywhere near full colonization. Unless the jar completely stalled out after you shook it, it should be good to go.
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whitelight7
Sr.
Registered: 02/17/09
Posts: 1,250
Loc: Washington
Last seen: 6 years, 9 months
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Re: I think this is yeast...could use some confirmation [Re: Brennus]
#12108026 - 02/27/10 01:06 PM (14 years, 1 month ago) |
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Looks fine, just some knots forming, too brilliant white to be yeast.
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Staunch supporter of spontaneous generation
 
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solumvita
Q.B.E.
Registered: 02/12/08
Posts: 2,061
Loc: South Africa
Last seen: 10 months, 4 days
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Re: I think this is yeast...could use some confirmation [Re: whitelight7]
#12112622 - 02/28/10 10:48 AM (14 years, 1 month ago) |
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that does not look like yeast to me either
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One of these days all the answers will be revealed until then we learn from each other!
www.mushrush.co.za
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Tony
Stranger
Registered: 09/25/09
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Re: I think this is yeast...could use some confirmation [Re: solumvita]
#12118307 - 03/01/10 06:03 AM (14 years, 1 month ago) |
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I was told this is yeast, which also made the mycelium act funny and rhizomorphic.
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Hofmann1943
explorer
Registered: 03/05/09
Posts: 409
Loc: Forest
Last seen: 4 years, 18 days
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Re: I think this is yeast...could use some confirmation [Re: Tony]
#12125790 - 03/02/10 05:04 AM (14 years, 1 month ago) |
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Quote:
Tony said:
I was told this is yeast, which also made the mycelium act funny and rhizomorphic.
who the fu*k told you that, we here like rhizomorphic mycelium don't we?
that's all good.
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By Albert Hofmann :
Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened.
That very small dosage, the first dose of my experiments i planed, was very very strong.
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Tony
Stranger
Registered: 09/25/09
Posts: 958
Last seen: 8 years, 5 months
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Re: I think this is yeast...could use some confirmation [Re: Hofmann1943]
#12125820 - 03/02/10 05:28 AM (14 years, 1 month ago) |
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It was RR himself who identified it as yeast 
It shows up as those circled patches of white dots, which appeared too early during the colonization to be hyphal knots(?). Also, growth of the mycelium seemed a somewhat stalled in places.
I'm a noob, but as far as I know, rhizomorphic growth can appear under different conditions, like when the substrate is a bit on the dry side. So, maybe the yeast acted as a stressor which caused the myc to go rhizomorphic, i.e. enter the berserk mode. Maybe that's what the rhizomorphic growth mode is - mycelium going berserk? 
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Brennus
Student of Life
Registered: 05/31/08
Posts: 3,297
Loc: SE United States
Last seen: 6 years, 1 month
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Re: I think this is yeast...could use some confirmation [Re: Tony]
#12126992 - 03/02/10 11:04 AM (14 years, 1 month ago) |
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Quote:
Tony said:
It was RR himself who identified it as yeast
It shows up as those circled patches of white dots, which appeared too early during the colonization to be hyphal knots(?). Also, growth of the mycelium seemed a somewhat stalled in places.
I'm a noob, but as far as I know, rhizomorphic growth can appear under different conditions, like when the substrate is a bit on the dry side. So, maybe the yeast acted as a stressor which caused the myc to go rhizomorphic, i.e. enter the berserk mode. Maybe that's what the rhizomorphic growth mode is - mycelium going berserk?
Never seen yeast do that before in my experience. Thanks for sharing.
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Hofmann1943
explorer
Registered: 03/05/09
Posts: 409
Loc: Forest
Last seen: 4 years, 18 days
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Re: I think this is yeast...could use some confirmation [Re: Tony]
#12128906 - 03/02/10 04:33 PM (14 years, 1 month ago) |
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Quote:
Tony said:
It was RR himself who identified it as yeast
It shows up as those circled patches of white dots, which appeared too early during the colonization to be hyphal knots(?). Also, growth of the mycelium seemed a somewhat stalled in places.
I'm a noob, but as far as I know, rhizomorphic growth can appear under different conditions, like when the substrate is a bit on the dry side. So, maybe the yeast acted as a stressor which caused the myc to go rhizomorphic, i.e. enter the berserk mode. Maybe that's what the rhizomorphic growth mode is - mycelium going berserk?
neither did I.
I think the rhizomorphic growth is possible if you have "clean" strain.
when you transport piece of mycelium from agar to agar you pick the most rhizomorphic piece. So that's what i want in my jar's.
Am i wrong?
that little white things are yeast,damn i never actually see one, I always smell it firs.
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By Albert Hofmann :
Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened.
That very small dosage, the first dose of my experiments i planed, was very very strong.
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whitelight7
Sr.
Registered: 02/17/09
Posts: 1,250
Loc: Washington
Last seen: 6 years, 9 months
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Re: I think this is yeast...could use some confirmation [Re: Hofmann1943]
#12131645 - 03/02/10 11:18 PM (14 years, 1 month ago) |
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yeah, but that was deff yeast in the highlighted area.
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Staunch supporter of spontaneous generation
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Hofmann1943
explorer
Registered: 03/05/09
Posts: 409
Loc: Forest
Last seen: 4 years, 18 days
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Re: I think this is yeast...could use some confirmation [Re: whitelight7]
#12132558 - 03/03/10 03:59 AM (14 years, 1 month ago) |
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it is great when you find out something new to you.
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By Albert Hofmann :
Been cautious man, I though I would start with a smallest, smallest quantity. Namely I started with 0.25mg.....and my intention was to increase dosage to see if something will happened.
That very small dosage, the first dose of my experiments i planed, was very very strong.
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MycoUnderground
Enthusiast
Registered: 10/23/08
Posts: 467
Loc: Suburban CO
Last seen: 12 years, 7 months
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Re: I think this is yeast...could use some confirmation [Re: Hofmann1943]
#12159423 - 03/07/10 02:15 PM (14 years, 1 month ago) |
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Well I let those jars sit for about 5 more days and the spots had grown some. Since I was going to spawn some other jars I decided I might as well check out the questionable ones. When I opened them up they just smelled like mycellium, so I figured I might as well spawn those too.
I had never seen growth like that before, so I was naturally very suspicious. But, if the odd growth spots were bacteria I think they would have become smelly after 10 days of being visible, not to mention the time before being visible.
Thanks for your inputs. I'll keep my fingers crossed and give an update in a few weeks.
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