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Amazon Shop: ½ Pint Jars, Agar, Potato Dextrose, Rye Grain

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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
PDA bacteria
    #1215231 - 01/13/03 05:59 PM (14 years, 7 months ago)

Last week I inoculated several pints of rye with a self made TC syringe. At the same time, I also inoculated 2 agar plates with the same syringe. All were sterilized in the same manner and inoculated in the same ghetto glovebox.

The rye jars are now showing healthy mycelial growth, but the two agar plates are growing nothing but bacteria. I can think of two possible explanations for this:

a) The syringe harbored a contam that favors PDA over rye.

b) The PDA is more easily contaminated and was infected during the inoculation process. Is a glovebox insufficient for working with agar?

Your opinions and advice would be appreciated. In case you could not tell this is my first experiment with agar.  :crazy:   


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


Edited by dog (01/14/03 04:43 AM)


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Invisibledeanofmean
mycophagous

Registered: 12/07/02
Posts: 2,017
Loc: PNW
Re: PDA bacteria [Re: dog]
    #1215344 - 01/13/03 06:45 PM (14 years, 7 months ago)

i am a newbie with agar, so im not qualified to give advice here .
but i am wondering, how many plates did you make, and did you incubate any before inoculation ?
also i would like to pass on this link which helped me, a lot .

http://www.shroomery.org/forums/showflat.php?Cat=&Board=Forum4&Number=1187975&page=2&view=collapsed&sb=5&o=&fpart=1

hope this helps .



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OfflineRaadt
nicht

Registered: 06/07/02
Posts: 2,104
Loc: azurescending
Last seen: 9 years, 7 months
Re: PDA bacteria [Re: dog]
    #1215666 - 01/13/03 08:29 PM (14 years, 7 months ago)

dirty tools, too early to tell in the rye, more easily contaminated, all could be reasons. You might want to try peroxidated agar, and cardboard.


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Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: PDA bacteria [Re: deanofmean]
    #1216367 - 01/14/03 04:49 AM (14 years, 7 months ago)

Quote:

how many plates did you make, and did you incubate any before inoculation ?



I made only two plates and did not incubate before inoculation. On my next attempt I will leave one plate uninoculated as a control. Thanks for the link.


--------------------


Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.


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Amazon Shop: ½ Pint Jars, Agar, Potato Dextrose, Rye Grain

Mushrooms, Mycology and Psychedelics >> Advanced Mycology

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