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Offlinerelapsd
odear
Registered: 01/12/03
Posts: 3
Last seen: 13 years, 6 months
new here- something i couldn't find in the FAQ
    #1212963 - 01/12/03 10:19 PM (14 years, 4 months ago)

hello. i'm a newb here. i couldn't find this question in the FAQ.

i was wondering if i had to have a fresh cap to develop a spore print.
is there any way i can create a spore print with the cap of a mushroom that has been dried?

i appreciate any help, thanks!
-michael


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OfflineGratefulDread
Never pass up anopportunity topee.

Registered: 10/21/02
Posts: 366
Last seen: 12 years, 8 months
Re: new here- something i couldn't find in the FAQ [Re: relapsd]
    #1212987 - 01/12/03 10:50 PM (14 years, 4 months ago)

    You could possibly take the black powder that is left from a bag of shrooms and rehydrate it in a syringe full of water and use that to innoculate jars but the chances that you would get no contaminants are very low. The spores that would be in the bag would be mixed with particles of the actual mushrooms which would contaminate your jars.

    Another way would be to rehydrate a cap in a cup of water and then draw that up into a syringe which would once again have a high probability of being contaminated as the shrooms have been handled and dried out in the open air so the cap is probably contaminated as well.

    You could theoretically do an agar isolation by allowing the spores to rehydrate and colonize agar and then cut away an uncontaminated sample of mycellium to start another plate then repeat the process until you have gotten rid of all contaminants. This would take about a month and a half, dozens of agar plates, a pressure cooker, and very sterile conditions so it's not really feasible.

    Or...  you could send a buck to the FSR in your area and get a print to start out with.

:smirk:


--------------------
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That way, when you criticize them, you're a mile away and you have their shoes.


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