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Shnezbit
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Registered: 09/30/04
Posts: 1,202
Loc: The Threshold.
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Spores on Agar: Green Mold, can I try again and transfer to H2o2 b4 the green?
#12109871 - 02/27/10 06:54 PM (13 years, 11 months ago) |
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Is this salvageable? Perhaps with the peroxide tech?
Edited by Shnezbit (02/27/10 11:50 PM)
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Shnezbit
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Registered: 09/30/04
Posts: 1,202
Loc: The Threshold.
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Re: Spores on Agar: Green Mold? [Re: Shnezbit]
#12111059 - 02/27/10 11:46 PM (13 years, 11 months ago) |
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I had the jar in the pic was started by droping spores from a print onto agar. These spores are like 3 years old I think(Been out of the hobby for the most part).
Yesterday I inocculated a jar of agar with spores from a syringe from that same print.
There appears white myc on the agar now, just a day later (These spores were from a Brazillian Cloned to liquid culture and was really agressive)
I'm thinking that syringe has active spores. Is there a way I could transfer the early myc to peroxide agar? Does anyone think it could work?
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Randomstickynote



Registered: 07/25/09
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Re: Spores on Agar: Green Mold? [Re: Shnezbit]
#12111199 - 02/28/10 12:15 AM (13 years, 11 months ago) |
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I only see mold growing in that jar, I don't see any mushroom mycelium unless it's hiding. The white that you see around the green is mold mycelium, the green that you see is mold spores. If there are tiny traces of mushroom mycelium in that glass, they're not worth salvaging due to the amount of mold spores floating around in there.
Try again definitely though, and try to catch the mushroom mycelium before the mold starts to sporulate if you get mold again. If you can get a little wedge of mycelium away, you might be able to get a clean culture in a couple of transfers.
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Shnezbit
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Registered: 09/30/04
Posts: 1,202
Loc: The Threshold.
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hi thanks. heres a pic of the newer jar started from a syring of the same spores dropped on the first pic jar:

theres no sign of the green in this one yet. was wondering if in theory i could just transfer a white spec to some peroxide agar.
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solumvita
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Re: Spores on Agar: Green Mold? [Re: Shnezbit]
#12112615 - 02/28/10 10:47 AM (13 years, 11 months ago) |
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try with multiple agar plates and isolate from there.
-------------------- One of these days all the answers will be revealed until then we learn from each other! www.mushrush.co.za
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suns_for_shadows
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Re: Spores on Agar: Green Mold? [Re: Shnezbit]
#12114420 - 02/28/10 04:03 PM (13 years, 11 months ago) |
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Quote:
Shnezbit said: hi thanks. heres a pic of the newer jar started from a syring of the same spores dropped on the first pic jar:

theres no sign of the green in this one yet. was wondering if in theory i could just transfer a white spec to some peroxide agar.
You could transfer it, and it would grow, but I wouldn't. Let it grow. Like your first picture, just because it's white, doesn't mean it's mycelium. It could very well be be more contams that haven't begun to produce spores yet. Wait it out, let it grow. Patience, It will show it's true colors eventually. No need to rush it.
What is your procedure?
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RogerRabbit
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I see no mushroom mycelium on your dish. You won't isolate mushroom mycelium from the cladisporium. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Shnezbit
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Registered: 09/30/04
Posts: 1,202
Loc: The Threshold.
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Re: Spores on Agar: Green Mold? [Re: RogerRabbit]
#12120872 - 03/01/10 02:38 PM (13 years, 11 months ago) |
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hmm..so basically theres no hope for those spores? you think the prints are likely infested with green mold spores or whatever it would be at that micro level? I have a bunch of prints from the same batch from a long time ago. I was hoping to resurect them.
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RogerRabbit
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Re: Spores on Agar: Green Mold? [Re: Shnezbit]
#12126208 - 03/02/10 08:04 AM (13 years, 11 months ago) |
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Try a different print, or a different section of that one. I see nothing you can isolate in that petri dish. Toss it out. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Shnezbit
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Registered: 09/30/04
Posts: 1,202
Loc: The Threshold.
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Re: Spores on Agar: Green Mold? [Re: RogerRabbit]
#12128152 - 03/02/10 02:32 PM (13 years, 11 months ago) |
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Thanks for the help. I did throw those 2 jars out. I was pretty disheartened But before I got disheartened I had inoculated another couple Agar jars with the Brazilian syringe. I didn't label my jars in time so I'm not sure if its the same Brazillian syringe or not Although I think this is different syringe from a different spore print from the same harvest about 3 years ago.
Well it's come along further than the other 2 jars so far and it's showing no signs of green and looks to be healthy myc to me, although the pictures don't do it clear enough for you to tell I really tried to get the best pictures but it comes out blurry through the glass and makes the myc look like mounds of white but in reality the white seems to be fluffy and spikey.
Also I noticed today for the first time that a Tazmanian jar I inoculated with the first batch is beginning to show growth too and so far no signs of green. This would really be amazing because that strain was really weak to begin with. It was from a harvest of old taz spores and the fruits were weak looking.
Anyway I'm new to working with agar and glove boxes and the concept of reviving a strain. Brazillian:
  Taz:
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