Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleShnezbit
Psycho-naught
Male User Gallery


Registered: 09/30/04
Posts: 1,202
Loc: The Threshold.
Spores on Agar: Green Mold, can I try again and transfer to H2o2 b4 the green?
    #12109871 - 02/27/10 06:54 PM (13 years, 11 months ago)



Is this salvageable? Perhaps with the peroxide tech?


Edited by Shnezbit (02/27/10 11:50 PM)


Extras: Filter Print Post Top
InvisibleShnezbit
Psycho-naught
Male User Gallery


Registered: 09/30/04
Posts: 1,202
Loc: The Threshold.
Re: Spores on Agar: Green Mold? [Re: Shnezbit]
    #12111059 - 02/27/10 11:46 PM (13 years, 11 months ago)

I had the jar in the pic was started by droping spores from a print onto agar. These spores are like 3 years old I think(Been out of the hobby for the most part).

Yesterday I inocculated a jar of agar with spores from a syringe from that same print.

There appears white myc on the agar now, just a day later (These spores were from a Brazillian Cloned to liquid culture and was really agressive)

I'm thinking that syringe has active spores. Is there a way I could transfer the early myc to peroxide agar? Does anyone think it could work?


Extras: Filter Print Post Top
OfflineRandomstickynote
Male User Gallery

Folding@home Statistics
Registered: 07/25/09
Posts: 824
Loc: TX Flag
Last seen: 3 years, 3 months
Re: Spores on Agar: Green Mold? [Re: Shnezbit]
    #12111199 - 02/28/10 12:15 AM (13 years, 11 months ago)

I only see mold growing in that jar, I don't see any mushroom mycelium unless it's hiding. The white that you see around the green is mold mycelium, the green that you see is mold spores. If there are tiny traces of mushroom mycelium in that glass, they're not worth salvaging due to the amount of mold spores floating around in there.

Try again definitely though, and try to catch the mushroom mycelium before the mold starts to sporulate if you get mold again. If you can get a little wedge of mycelium away, you might be able to get a clean culture in a couple of transfers.


--------------------
AMU


Extras: Filter Print Post Top
InvisibleShnezbit
Psycho-naught
Male User Gallery


Registered: 09/30/04
Posts: 1,202
Loc: The Threshold.
Re: Spores on Agar: Green Mold? [Re: Randomstickynote]
    #12111317 - 02/28/10 12:54 AM (13 years, 11 months ago)

hi thanks. heres a pic of the newer jar started from a syring of the same spores dropped on the first pic jar:


theres no sign of the green in this one yet. was wondering if in theory i could just transfer a white spec to some peroxide agar.


Extras: Filter Print Post Top
Offlinesolumvita
Q.B.E.
Male User Gallery

Registered: 02/12/08
Posts: 2,061
Loc: South Africa
Last seen: 8 months, 13 days
Trusted Cultivator
Re: Spores on Agar: Green Mold? [Re: Shnezbit]
    #12112615 - 02/28/10 10:47 AM (13 years, 11 months ago)

try with multiple agar plates and isolate from there.


--------------------
One of these days all the answers will be revealed until then we learn from each other!

www.mushrush.co.za


Extras: Filter Print Post Top
Offlinesuns_for_shadows
Wanderer


Registered: 05/06/09
Posts: 49
Last seen: 10 years, 8 months
Re: Spores on Agar: Green Mold? [Re: Shnezbit]
    #12114420 - 02/28/10 04:03 PM (13 years, 11 months ago)

Quote:

Shnezbit said:
hi thanks. heres a pic of the newer jar started from a syring of the same spores dropped on the first pic jar:


theres no sign of the green in this one yet. was wondering if in theory i could just transfer a white spec to some peroxide agar.




You could transfer it, and it would grow, but I wouldn't. Let it grow. Like your first picture, just because it's white, doesn't mean it's mycelium. It could very well be be more contams that haven't begun to produce spores yet. Wait it out, let it grow. Patience, It will show it's true colors eventually. No need to rush it.

What is your procedure?


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 4 days
Trusted Cultivator
OG Cultivator
Re: Spores on Agar: Green Mold? [Re: suns_for_shadows]
    #12116052 - 02/28/10 07:45 PM (13 years, 11 months ago)

I see no mushroom mycelium on your dish.  You won't isolate mushroom mycelium from the cladisporium.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
InvisibleShnezbit
Psycho-naught
Male User Gallery


Registered: 09/30/04
Posts: 1,202
Loc: The Threshold.
Re: Spores on Agar: Green Mold? [Re: RogerRabbit]
    #12120872 - 03/01/10 02:38 PM (13 years, 11 months ago)

hmm..so basically theres no hope for those spores? you think the prints are likely infested with green mold spores or whatever it would be at that micro level? I have a bunch of prints from the same batch from a long time ago. I was hoping to resurect them.


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery

Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 4 days
Trusted Cultivator
OG Cultivator
Re: Spores on Agar: Green Mold? [Re: Shnezbit]
    #12126208 - 03/02/10 08:04 AM (13 years, 11 months ago)

Try a different print, or a different section of that one.  I see nothing you can isolate in that petri dish.  Toss it out.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
InvisibleShnezbit
Psycho-naught
Male User Gallery


Registered: 09/30/04
Posts: 1,202
Loc: The Threshold.
Re: Spores on Agar: Green Mold? [Re: RogerRabbit]
    #12128152 - 03/02/10 02:32 PM (13 years, 11 months ago)

Thanks for the help. I did throw those 2 jars out. I was pretty disheartened :frown: But before I got disheartened I had inoculated another couple Agar jars with the Brazilian syringe. I didn't label my jars in time so I'm not sure if its the same Brazillian syringe or not :frown: Although  I think this is different syringe from a different spore print from the same harvest about 3 years ago.

Well it's come along further than the other 2 jars so far and it's showing no signs of green and looks to be healthy myc to me, although the pictures don't do it  clear enough for you to tell :frown: I really tried to get the best pictures but it comes out blurry through the glass and makes the myc look like mounds of white but in reality the white seems to be fluffy and spikey.

Also I noticed today for the first time that a Tazmanian jar I inoculated with the first batch is beginning to show growth too and so far no signs of green. This would really be amazing because that strain was really weak to begin with. It was from a harvest of old taz spores and the fruits were weak looking.

Anyway I'm new to working with agar and glove boxes and the concept of reviving a strain.
Brazillian:

Taz:


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   Unfolding Nature Unfolding Nature: Being in the Implicate Order   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Liquid Cultures   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* Green Mold...CRAP! (PICS) Need Advice Birdman 12,664 8 03/06/03 01:04 PM
by Birdman
* Green mold? *DELETED* poke smot! 1,701 4 01/14/03 01:21 PM
by Anno
* Green Mold Aussieland 2,259 5 10/31/01 07:48 PM
by Zen Peddler
* GREEN MOLD ON ALL MY JARS, HELP!!!!!! Supershrooomer 4,203 5 05/16/19 05:13 AM
by theozaki
* green mold scenario twistedweather 2,602 3 05/20/03 07:37 PM
by twistedweather
* Grey/green mold on casing - PIC Partysan 4,190 4 06/14/02 10:27 PM
by We_come_in_peace
* Does Green mold start white? SubGen1us 1,781 8 12/16/02 03:54 PM
by blahblahblah
* 90% green molds are NOT Trichoderma. Zen Peddler 7,538 5 04/02/02 01:39 PM
by Seuss

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
3,027 topic views. 34 members, 177 guests and 25 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.008 seconds on 12 queries.