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OfflineFcuerkt
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starting from a print
    #1211385 - 01/12/03 09:32 AM (14 years, 2 months ago)

I got a print from FSRE. Can I scrape the spores directly into the jars, or do I need to put them into a syringe, or colonize some water, or what? Thanks in advance, sorry if this question is too elementary


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Offlinegeo2001m
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Re: starting from a print [Re: Fcuerkt]
    #1211403 - 01/12/03 09:46 AM (14 years, 2 months ago)

the faq has answers better than what i can explain


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OfflineMacey Howard
Formally MOE HOWARD
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Post deleted by Moe Howard [Re: Fcuerkt]
    #1211410 - 01/12/03 09:52 AM (14 years, 2 months ago)



--------------------
Hugs and Kisses!


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Invisiblefunkymonk
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Re: starting from a print [Re: Macey Howard]
    #1211412 - 01/12/03 09:55 AM (14 years, 2 months ago)

Scrap the spore's into a shot glass, use sterile water, and suck up into a syringe...


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OfflineFcuerkt
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Re: starting from a print [Re: Fcuerkt]
    #1211414 - 01/12/03 09:56 AM (14 years, 2 months ago)

I did.

"You can either make a syringe and use this inoculate pf cakes, transfer the spores to agar medium or directly to sterilized grain substrates. "

Does this mean that I CAN scrape the spores directly into the jars? I only have one print and I'm not anxious to screw it up.


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Invisibledeanofmean
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Registered: 12/07/02
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Re: starting from a print [Re: Fcuerkt]
    #1211454 - 01/12/03 10:11 AM (14 years, 2 months ago)

the simplest, easiest way would be to make a test syringe with a weak dextrose or sucrose solution first .let the spores germinate in the syringe, and wait a few days to see if any contams show up .
and, yes go to faq and read, read, read !


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OfflineAnnoA
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Re: starting from a print [Re: Fcuerkt]
    #1211493 - 01/12/03 10:38 AM (14 years, 2 months ago)

>Does this mean that I CAN scrape the spores directly into the jars? I only have
>one print and I'm not anxious to screw it up.

Yes, BUT:
- you will need a shakeable substrate/tye, millet, corn...
- you will need some kind of a sterile working space, so when you open the jar, air from outside doesn?t enter and contaminate the jar(glovebox, flowhood...)

I?d suggest that you follow the Psilocyber?s syringe tek.
Then inoculate PF-jars with the syringe(s).

Depending on the size of the print, you can make 5-10 good syringes from one print.


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