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InvisibleAlien
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Registered: 11/14/99
Posts: 1,868
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Post deleted by Administrator [Re: Baby_Hitler]
    #1207725 - 01/10/03 05:02 PM (21 years, 2 months ago)



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OfflineAnnoA
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Re: Popcorn as grain substrate? [Re: Alien]
    #1208324 - 01/11/03 12:40 AM (21 years, 2 months ago)

To add to the general corn-craze, I?m testing corn semolina instead of BRF in PF cakes.
On the left is a substrate with BRF and millet, on the right with corn semolina.


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InvisibleHippie3
mycotopiate
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Registered: 11/06/99
Posts: 3,090
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Re: Popcorn as grain substrate? [Re: EthnoJimmy]
    #1208329 - 01/11/03 12:54 AM (21 years, 2 months ago)

i also just used tapwater.
as for nutrition, a member of mycotopia's forums posted this:
Nutritional Data for 100 grams of POPCORN, AIR-POPPED
Mean value per 100.00 grams edible part; 0.0% refuse
Portions: 1 OZ = 28.35 gm, 1 C = 8.00 gm
10.1% Cals from fat, 13.1% Cals from protein, 76.7% Cals from carbs.
Male Female
Name Unit Amount %RDA %RDA
Food energy KCal: 382.000 13.2% 17.4%
Protein Gms : 12.000 19.0% 24.0%
Total lipid (fat) Gms : 4.200 4.3% 5.7%
Carbohydrate, by diff. Gms : 77.900 16.6% 23.3%
Total saturated fat Gms : 0.570 1.8% 2.3%
Ttl monounsaturated fat Gms : 1.100
Ttl polyunsaturated fat Gms : 1.900
Cholesterol Mg : 0.000 0.0% 0.0%
Sodium Mg : 4.000 0.8% 0.8%
Total dietary fiber Gms : 15.100 60.4% 60.4%
Vitamin A Re : 20.000 2.0% 2.5%
Vitamin A IU : 196.000
Ascorbic acid Mg : 0.000 0.0% 0.0%
Thiamin Mg : 0.203 13.5% 18.5%
Riboflavin Mg : 0.283 16.6% 21.8%
Niacin Mg : 1.944 10.2% 13.0%
Vitamin B6 Mg : 0.245 12.2% 15.3%
Folacin Mcg : 23.000 11.5% 12.8%
Vitamin B12 Mcg : 0.000 0.0% 0.0%
Potassium Mg : 301.000 15.1% 15.1%
Calcium Mg : 10.000 1.2% 1.2%
Phosphorus Mg : 300.000 37.5% 37.5%
Magnesium Mg : 131.000 37.4% 46.8%
Iron Mg : 2.660 26.6% 17.7%
Zinc Mg : 3.440 22.9% 28.7%
Pantothenic acid Mg : 0.420 8.4% 8.4%
Copper Mg : 0.420 21.0% 21.0%
Manganese Mg : 0.943 26.9% 26.9%
Ash Gms : 1.800
Water Gms : 4.100
Food energy KJ : 1598.000
Palmitic acid (16:0) Gms : 0.500
Stearic acid (18:0) Gms : 0.070
Palmitoleic acid(16:1) Gms : 0.000
Oleic acid (18:1) Gms : 1.100
Linoleic acid (18:2/n6) Gms : 1.840 28.8% 37.6%
Linolenic acid(18:3/n3) Gms : 0.060 3.8% 5.0%
Histidine Gms : 0.367 38.6% 48.3%
Isoleucine Gms : 0.431 54.6% 68.4%
Leucine Gms : 1.473 132.7% 167.4%
Lysine Gms : 0.338 35.6% 44.5%
Methionine Gms : 0.252
Cystine Gms : 0.217
Methionine+Cystine Gms : 0.469 45.5% 57.2%
Phenylalanine Gms : 0.590
Tyrosine Gms : 0.488
Phenylalanine+Tyrosine Gms : 1.078 97.1% 122.5%
Threonine Gms : 0.452 82.2% 51.4%
Tryptophan Gms : 0.085 30.4% 38.6%
Valine Gms : 0.607 76.8% 96.3%
Arginine Gms : 0.598
Alanine Gms : 0.900
Aspartic acid Gms : 0.836
Glutamic acid Gms : 2.255
Glycine Gms : 0.492
@Y: 1.048
Serine Gms : 0.571
Protein Score: 100, 43% ideal. EAA score: 1.59.
Limiting Amino Acid: Tryptophan


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Offlinecalishroomer
Looking For Lunatic In TheGrass!

Registered: 12/28/02
Posts: 117
Last seen: 21 years, 1 month
Re: Popcorn as grain substrate? [Re: Baby_Hitler]
    #1208384 - 01/11/03 02:35 AM (21 years, 2 months ago)

"That was before they had a website..
Anyways sparks is the inventor of the TEK and yes its been around for a while, i think ralphster can verify the TEK from the now non existant forum NSS..[Nordic Shroom Service."



I belive always that credit should be given were do. BUT the fact remains that whomever cane up with this wonderful idea should be applauded...i can see alot of benifits of using popcorn for sure. sounds like its easiest as you dont have to mix a ratio of differebt items togeather to achieve the desired result AND its alot easier to comeby for most, including newbeez (everyone has popcorn in there cabnits , well almost)

great thread!!!.....5 shroomer!~!~!~!~!~!~




DISCLAIMER:

As a Registered Donor of TheShroomery ,the information contained in any of my posts relating to Mushroom hunting and Identification is information that I have gathered from publicly known sources correspondingly under the same protections of Free Speech governed under the Laws of the United States and Canada and is for informational use only. As my Constitutional Right under United States and Canadian Laws the redistribution of said information is considered a form of free speech. Using this information in the United States or Canada to Illegally prosess or identify any sort of illegal substance or chemical were has been declared against the law in those countries, and as such I do not advocate the illegal use of such information under both the United States and Canadian Laws. All references and examples of personal experiences are hypothetical in nature, and it is your responsibility to know whether information contained within this post is legal according to applicable laws in the country of usage. I will not be held responsible for how you use this information, nor will i be held responsible for any legal problems incurred by you ( the reader).USE THIS INFORMATION WISELY!


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"The Lunatic Is In The Grass!!"

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InvisibleHippie3
mycotopiate
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Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
Re: Popcorn as grain substrate? [Re: Alien]
    #1208442 - 01/11/03 03:48 AM (21 years, 2 months ago)

alien,
i am still using the portable/wardrobe/closet that you mention.


plastic portable wardrobe closet $25 at walmart
plastic shelving $20
hepa air filter and centrifugal cool mist humidifier run off a timer/power strip to regulate humidity
seems to work pretty good
the thread at mycotopia that inspired me is HERE


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Admin @ mycotopia.net
Mycotopia

Edited by Hippie3 (01/11/03 03:54 AM)

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InvisibleHippie3
mycotopiate
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Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
Re: Popcorn as grain substrate? [Re: Anno]
    #1208445 - 01/11/03 03:51 AM (21 years, 2 months ago)

anno,
corn 'semolina' is exactly what ?
what formula did you use ?
any growth yet ?


--------------------
Admin @ mycotopia.net
Mycotopia

Edited by Hippie3 (01/11/03 10:27 AM)

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OfflineAnnoA
Experimenter
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Posts: 24,166
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Re: Popcorn as grain substrate? [Re: Hippie3]
    #1208459 - 01/11/03 04:09 AM (21 years, 2 months ago)

Corn semolina.
Semolina is.....well, semolina. It looks like a coarse corn flour.
I used the PF-Tek for Simple Minds with 140 ml vermiculite and 40 ml corn semolina.
I just inoculated it today.


The "trure" semolina is actually made of wheat:

Semolina
A creamy white powder that is made by sifting the hard part of the wheat from flour.
Uses: It is used to make the traditional British semolina pudding which is a smooth, creamy pudding - the semolina is cooked slowly in milk, sweetened with sugar and flavoured with vanilla and sometimes enriched with egg. Semolina pudding can be served with raisins, currants or sultanas stirred in or with a dollop of jam. Semolina is also the main ingredient for making pasta and can be added to biscuit, cake and bread doughs. The traditional Italian dumplings, gnocchi, can be made with semolina or mashed potato.


Semolina is coarsely ground durum wheat, a highly glutinous (hard) wheat. When other grains, such as rice or corn, are similarly ground, they are referred to as "semolina," i.e., "corn semolina" or "rice semolina."
http://www.cooking.com/advice/adgloss.asp?GlossType=ingr&Item=Semolina

Edited by Anno (01/11/03 09:50 AM)

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InvisibleAlien
Galactic Shaman
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Registered: 11/14/99
Posts: 1,868
Loc: Jupiter's Moon
Post deleted by Administrator [Re: Hippie3]
    #1208503 - 01/11/03 04:54 AM (21 years, 2 months ago)



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Offlinec0ma
the pimp

Registered: 09/22/02
Posts: 1,249
Last seen: 18 years, 2 months
Re: Popcorn as grain substrate? [Re: Alien]
    #1208882 - 01/11/03 07:54 AM (21 years, 2 months ago)

can inculation be down with a spore print usin oven tek?
i was planning if i could scrape some spores inside the grain jar with a sterile knife?

also what kinda popcorn will work? can i just buy regular popcorn from the store like food mart, yellow or white? what brand works best ?

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Offlineindicaz
Lysol God
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Registered: 04/12/02
Posts: 580
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Re: Popcorn as grain substrate? [Re: c0ma]
    #1208960 - 01/11/03 08:27 AM (21 years, 2 months ago)

Methods of innoculation please...

Obviously Agar to Grain Would work...

Could I take a PK cake and use that to innoculate via a transfer?

Could someone theoretically just use a syringe to innoculate?

What has worked best for everyone???

Laterz

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Offlinec0ma
the pimp

Registered: 09/22/02
Posts: 1,249
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Re: Popcorn as grain substrate? [Re: indicaz]
    #1208969 - 01/11/03 08:32 AM (21 years, 2 months ago)

help!!! while i still have money for popcorn and some water :smile:

(look) above 2 posts up...

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InvisibleBilge
longtimenoC

Registered: 08/26/02
Posts: 1,858
Loc: USA
Re: Popcorn as grain substrate? [Re: c0ma]
    #1208984 - 01/11/03 08:38 AM (21 years, 2 months ago)

you should be able to inoculate using a spore print, yes. oven tek may work, but glove box or flow hood would be better. this is just another grain tek, so research that for your answers.

i would guess that any non-microwave popcorn would work. you don't want microwave popcorn in the microwave bags because there is other junk mixed in. just use the standard cheap plastic bags of popcorn.


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InvisibleHippie3
mycotopiate
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Registered: 11/06/99
Posts: 3,090
Loc: mycotopia.net
Re: Popcorn as grain substrate? [Re: indicaz]
    #1209252 - 01/11/03 10:24 AM (21 years, 2 months ago)

Quote:

Could someone theoretically just use a syringe to innoculate?



yep, that's how i did it, about the easiest way there is, too, imo.
the oven door tek is bogus, build a real glovebox.
any color popcorn should work, but i've been using white 'cause it's cheaper where i shop.


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Offlineshroomerman
dopaminecatalyst

Registered: 09/16/02
Posts: 397
Loc: florida
Last seen: 21 years, 1 month
Re: Popcorn as grain substrate? [Re: Hippie3]
    #1209851 - 01/11/03 03:26 PM (21 years, 2 months ago)

I have just completed the PC-ing of the popcorn jars phase. I used 3 different sized jars,(because that's what I had on hand) half pint, pint, and quart. I let the corn drain well, and even poured out the corn onto a towel (after the boiling/soaking) to get up all the excess water I could. Still I am noticing much condensation in the jars as they come out of the cooker. Is this normal ? Is this OK ? I plan on innoculating with a standard syringe innoculation. I'll let you know how it goes. i hope I don't get all contams because of fucking up with the water content somehow. Please help I am a noob. Peace. :grin: 


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Edited by shroomerman (01/12/03 05:14 AM)

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InvisibleHippie3
mycotopiate
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Registered: 11/06/99
Posts: 3,090
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Re: Popcorn as grain substrate? [Re: shroomerman]
    #1210818 - 01/11/03 10:09 PM (21 years, 2 months ago)

some condensation is normal, esp. if still warm from the cooker. moisture content seems pretty hard to over-do with popcorn so far, so keep the faith.


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Offlineshroomerman
dopaminecatalyst

Registered: 09/16/02
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Last seen: 21 years, 1 month
Re: Popcorn as grain substrate? [Re: Hippie3]
    #1211214 - 01/12/03 05:22 AM (21 years, 2 months ago)

Another quickie question please. I PC-ed the jars with the self sealing lids on them. They are now vacuum sealed. I plan on using a syringe and poking a hole in the lids to innoculate, then taping the holes shut before placing in an incubator. Is this ok? I have been reading, and I am seeing mentions of "filter discs", and "tyrek". Should I have put some sort of  filter disks on the jars before PCing? After PCing? Will I get a lot of contams by just doing as I stated above? Please tell me what I should do, I don't wan't to screw this up. I'll listen and do what you say to do to save my project from noob-inflicted disaster. Thanks. :laugh:


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InvisibleBilge
longtimenoC

Registered: 08/26/02
Posts: 1,858
Loc: USA
Re: Popcorn as grain substrate? [Re: shroomerman]
    #1211225 - 01/12/03 05:33 AM (21 years, 2 months ago)

grain needs more air exchange than brf. you need to have a hole in the jar (lid). generally there is a hole filled with polyfill, a filter disc or tyvek.

http://shroomery.org/faq/faq.php?display=faq&nr=70&catnr=5&prog=1&lang=en


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InvisibleHippie3
mycotopiate
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Posts: 3,090
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Re: Popcorn as grain substrate? [Re: shroomerman]
    #1211264 - 01/12/03 06:06 AM (21 years, 2 months ago)

you have a problem.
when you poke a hole in the lids,
it's gonna suck air into the jars.
since there's no filter in place to equalize the pressure or filter out any incoming contams.
so you have to poke the holes while working inside a decent glovebox.
and don't just stick a syringe thru the lid, the vacume will suck the entire syringe in faster than you can stop it.
so next time poke your holes and put your filter in position before you pc the grains.


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Offlineshroomerman
dopaminecatalyst

Registered: 09/16/02
Posts: 397
Loc: florida
Last seen: 21 years, 1 month
Re: Popcorn as grain substrate? [Re: Hippie3]
    #1211377 - 01/12/03 07:22 AM (21 years, 2 months ago)

Hmmmm. Ok I am thinking, perhaps I can just start over with the same popcorn. Open up the jars, get some of that excess moisture out of them, and re-do them correctly with filters, then PC them over again. I am somewhat at a loss for where to find the proceedures for using the filters. Are they circles of material that are the size of the metal lids? Do they go under the regular metal lids, or are they used alone? I'd love to see a diagram of how they are supposed to go, please link me to some reading materials or help explain. Also, I need to know where I could go purchase a suitable filter material? Please help, and thanks for the much help already given. 5 Mushrooms comin' .Peace :laugh:


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InvisibleBilge
longtimenoC

Registered: 08/26/02
Posts: 1,858
Loc: USA
Re: Popcorn as grain substrate? [Re: shroomerman]
    #1211436 - 01/12/03 08:03 AM (21 years, 2 months ago)

there are two ways, *both* requiring at least a small (3/8 inch) hole in the lid: either stuff the hole with polyfill (polyester filling used to stuff pillows) or cover the mouth of the jar with a filter material (filter disc or tyvek) before putting the lid on.

i use tyvek. i get tyvek by going to office depot and buying a box of dupont tyvek envelopes. then i cut a square out that is large enough so that when i put it on the mouth of the jar and screw down the lid there is still some sticking out all around the lid. then i take an exacto knife and cut the tyvek close to the edge of the lid. so i end up with a lid with a hole in it screwed onto the tyvek and onto the jar. i cover the lid end of the jar in aluminum foil and pc.

after pc'ing and after the pressure drops but before the jars are cool, i remove them from the pc and shake them to mix wet/dry grains. then i put them back in the pc, put the pc lid back on and leave them to cool over night. when i'm ready for inoculation, i remove the foil and throw it away. i drill my hole far to one side of the lid, so when i loosen the lid i can turn it so the hole is on one side of the lid. i inoculate through the hole, through the tyvek layer and into the jar. then i turn the lid so the hole is on the opposite side and screw the lid on tight.


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