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InvisibleburtonRebel
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Registered: 12/02/06
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sterilize mycelium from lc
    #12055661 - 02/18/10 05:48 PM (14 years, 10 months ago)

Hi, i am having problems innoculating brf with lc from wild sporeprints. i am curious if it is possible to isolate pieces of the mycelium from the lc and sterilize them without harming it. i was thinking a quick dunk in a light bleach solution or rubbing alcohol, adding to distilled water, and then making a syringe.


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InvisibleJonEveryman88
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Registered: 01/18/10
Posts: 1,638
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Re: sterilize mycelium from lc [Re: burtonRebel]
    #12055684 - 02/18/10 05:53 PM (14 years, 10 months ago)

You'd have more success isolating a contam with agar.

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Invisiblemyceleus_rex
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Registered: 07/01/09
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Re: sterilize mycelium from lc [Re: JonEveryman88]
    #12055786 - 02/18/10 06:14 PM (14 years, 10 months ago)

That's best, or cloning the wild specimens rather than printing them. Agar is still the best route, but with tissue cloning, at least you have a fighting chance going to grain or LC.


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Invisiblepassenger
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Registered: 11/05/08
Posts: 337
Re: sterilize mycelium from lc [Re: burtonRebel]
    #12055821 - 02/18/10 06:19 PM (14 years, 10 months ago)

Quote:

burtonRebel said:
Hi, i am having problems innoculating brf with lc from wild sporeprints. i am curious if it is possible to isolate pieces of the mycelium from the lc and sterilize them without harming it. i was thinking a quick dunk in a light bleach solution or rubbing alcohol, adding to distilled water, and then making a syringe.




id put those spores on agar and transfer out the good contam free mycelium to fresh plates until you have a clean plate then make an lc then inoculate you're jars. it's the only sure way.

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InvisibleburtonRebel
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Registered: 12/02/06
Posts: 1,222
Re: sterilize mycelium from lc [Re: passenger]
    #12055899 - 02/18/10 06:32 PM (14 years, 10 months ago)

alright, i guess i'll ditch the lc in some wood chips somewhere and hope for automatic bio-remediation. thanks for your replies.


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