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OfflineLogicaL ChaosM
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What To Do After Casing a Grain Jar: Fruiting Chamber or Incubate in Darkness?
    #12052279 - 02/18/10 06:48 AM (14 years, 10 months ago)

I've been searching for about an hour in older postings and I haven't really found what I was looking for. Its eithier there's a conflict on the best procedure to do, or I just didn't find any credible information to follow, so I'm trying here....

Right now, I have some 100% colonized (P. cubenis) wheat berries that I just cased 2 days ago with straight 100% vermiculite that was sterilized in the oven at some high temp (no temp gauge) for 1 hour. Then it was cooled for about 2 hours, sprayed the vermiculite with water mixed with H2O2 at a ratio of 1 fluid oz. H2O2 to 24 fluid Oz. filtered water. Then the wet vermiculite and jar of wheat berries went into my glovebox, where the jar was empyted into a metal cooking pan (which was sterilized while the vermiculite was sterilized). Next, while still in the semi-sterile glovebox, I put a layer of vermiculite into the pan ab out 3/4" deep.

Like So....


Lastly, I covered the pan with aluminum foil and poke small holes in the top for FAE and placed it into my newly built "shotgun fruiting chamber", which is at a silly 99% rH from the perlite (too high for cases). I tried to drop the humidity to 70% using glass pint jars put onto the perlite, and by placing the pan directly onto of the perlite, but it didn't help at all. How do I drop the rH without introducing infections to my casing attempt?

Here's it in the fruiting chamber, Day 2....

^I have not used my Flouro Light yet, because I read somewhere that will cause too early inapporiate pinning....

Anyway, what's the best thing to do after casing a grain jar: put it inside a FC with a humidity of 70% and a temp range of 72F-78F or put it into a dark, incubation chamber of temps like 78F-82F with no light and 70% humidity?

Thanks for any help!

P.S. I didn't use a better, more complex casing because I failed last time with the pasturization process, so I'm simplifying it to the basics....I am well aware that a 100% vermiculite casing is *not* the optimum recipe for high-yield casing, but I'm doing this attempt as an experiment to see how well (or poor) it performs as a casing....

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Re: What To Do After Casing a Grain Jar: Fruiting Chamber or Incubate in Darkness? [Re: LogicaL Chaos]
    #12052288 - 02/18/10 06:52 AM (14 years, 10 months ago)

i would just leave it on a shelf at room temp
you want the grains to recover before you introduce to fruiting conditions
once you see myc poking the casing layer then fruit it

    -noobie-


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Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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Re: What To Do After Casing a Grain Jar: Fruiting Chamber or Incubate in Darkness? [Re: noobieshroomie]
    #12052437 - 02/18/10 07:40 AM (14 years, 10 months ago)

There is also no need to drop the humidity.  99% is great, especially with all the fresh air you'll get in a shotgun terrarium.  There's a lot of horrible information floating around on humidity.  A cased substrate can 'tolerate' a lower humidity than cakes, but that doesn't mean a cased tray 'should' be put into lower humidity.  Mushrooms form best in near saturation humidity with lots of fresh air.
RR


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OfflineLogicaL ChaosM
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Re: What To Do After Casing a Grain Jar: Fruiting Chamber or Incubate in Darkness? [Re: RogerRabbit]
    #12055520 - 02/18/10 05:27 PM (14 years, 10 months ago)

Thanks mR. Rabbit for clearing up my confusion on humidity and casings. I kept reading in different places that casings, quote, "cannot" fruit in 99% humidity conditions like cakes, but will fruit in 75%-85% rH, because the casing material is already damp with water or something and "does not" require high humidity. At least, thats the common argument I have read.

So RR, you're saying that is actually benefical to put a just-cased layer of colonized grains into 99%-100% humidity enviroment because mushrooms love near saturation humidity levels and lots of fresh air. Alright! :laugh::thumbup:

I guess that means I should remove the upside pint jars I have covering the perlite, because thats just slowing down the FAE on the bottom of my Shotgun Chamber.

Well, good to know! I'll be coming back later with photos to share when the casing is covered with mycelium.

Till then, this is I, signing off....

Thanks for stoppin' by mR. Rabbit
~ LogicaL Chaos ~

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