Home | Community | Message Board

MRCA Tyroler Gluckspilze
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Left Coast Kratom Buy Kratom Extract   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   Original Sensible Seeds Autoflowering Cannabis Seeds   Kraken Kratom Red Vein Kratom   Bridgetown Botanicals CBD Concentrates   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinece_greg
Stranger

Registered: 09/25/06
Posts: 43
Last seen: 12 years, 2 days
culture syringe from grain
    #12054059 - 02/18/10 01:25 PM (14 years, 10 months ago)

I have a jar of rye 1/2 colonized, its my only jar thats colonizing so its pretty precious.

I have loads more jars that need inoculating and I'm debating whether or not to do a grain exchange or to try and make a culture syringe from  a piece of colonized grain in the same manner that one would make a mycelium syringe from a slice of mycelium from a petri dish.

I have a few spores left but I have diluted the syringe to the point where I feel I would be adding excessive water to my jars just to get a single spore in... or maybe not.

I'm going to try inoculating with the spore syringe and see what happens, but failing that would I be able to make a mycelium syringe with colonized rye or should I just try adding colonized rye to each jar instead?

I also had another idea which i'm trying to put into motion. I made up some jars of bird seed out of those really small jars that you get dried herbs in.
I want to wait for it to colonize then get a syringe full of sterile water, shoot it all into the colonized jar, give it a good shake and extract the water back into the syringe.
It seems like a good method in theory, so I'll report back and see how that goes.

adios

Extras: Filter Print Post Top
OfflineDamion5050
Mush Doctor
Male User Gallery

Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation ! Flag
Last seen: 3 years, 11 months
Trusted Cultivator
Re: culture syringe from grain [Re: ce_greg]
    #12054077 - 02/18/10 01:28 PM (14 years, 10 months ago)

Look up grain LC by agar I think it is..

Second I would not use any jar that isnt 100%

Extras: Filter Print Post Top
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: culture syringe from grain [Re: Damion5050]
    #12054081 - 02/18/10 01:29 PM (14 years, 10 months ago)

Quote:

Damion5050 said:
Look up grain LC by agar I think it is..

Second I would not use any jar that isnt 100%



:thumbup:
exactly wat i was thinking


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Offlinece_greg
Stranger

Registered: 09/25/06
Posts: 43
Last seen: 12 years, 2 days
Re: culture syringe from grain [Re: truskool]
    #12054291 - 02/18/10 02:03 PM (14 years, 10 months ago)

yeah, that would make sense actually, especially considering its my only jar thats wanting to do anything.

I guess I'll be sorting out the woodlovers tonight then...

Extras: Filter Print Post Top
Invisiblemyceleus_rex
seeker
Male User Gallery


Registered: 07/01/09
Posts: 1,581
Loc: alized
Re: culture syringe from grain [Re: ce_greg]
    #12054353 - 02/18/10 02:15 PM (14 years, 10 months ago)

Agar's tek is right in my sig.
:goodluck:


--------------------
Cervantes' sclerotia thread    Citric's self-healing lid tek  Agar's Grain LC  Breaking Up Spawn Jars

World's Ugliest Cat?

If ignorance is bliss, why aren't there more happy people?

Extras: Filter Print Post Top
OfflineJiggle
Peanut Herder
Male


Registered: 01/13/10
Posts: 360
Loc: The South
Last seen: 11 years, 7 months
Re: culture syringe from grain [Re: myceleus_rex]
    #12054837 - 02/18/10 03:34 PM (14 years, 10 months ago)

G2G, you don't need 100% for that plus it seems harder to screw up a G2G than an LC...

Make a grain lc when you got a few more jars

Extras: Filter Print Post Top
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: culture syringe from grain [Re: Jiggle]
    #12054870 - 02/18/10 03:40 PM (14 years, 10 months ago)

But why not do a GLC then when it regrows do g2g?  Thats wat I would do in the situation


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
OfflineJiggle
Peanut Herder
Male


Registered: 01/13/10
Posts: 360
Loc: The South
Last seen: 11 years, 7 months
Re: culture syringe from grain [Re: truskool]
    #12054897 - 02/18/10 03:45 PM (14 years, 10 months ago)

Only because I find it easier to screw up a glc than to screw up a g2g. And in this situation where it's your last chance before you have to buy more spores I would just prefer to take the safest route.

Extras: Filter Print Post Top
Invisiblemyceleus_rex
seeker
Male User Gallery


Registered: 07/01/09
Posts: 1,581
Loc: alized
Re: culture syringe from grain [Re: Jiggle]
    #12054944 - 02/18/10 03:54 PM (14 years, 10 months ago)

Quote:

Jiggle said:
Only because I find it easier to screw up a glc than to screw up a g2g. And in this situation where it's your last chance before you have to buy more spores I would just prefer to take the safest route.



You really think so? You don't even have to work with any open jars to do a GLC.

In either case, I'd wait, if possible, for full colonization.


--------------------
Cervantes' sclerotia thread    Citric's self-healing lid tek  Agar's Grain LC  Breaking Up Spawn Jars

World's Ugliest Cat?

If ignorance is bliss, why aren't there more happy people?

Extras: Filter Print Post Top
OfflineJiggle
Peanut Herder
Male


Registered: 01/13/10
Posts: 360
Loc: The South
Last seen: 11 years, 7 months
Re: culture syringe from grain [Re: myceleus_rex]
    #12055025 - 02/18/10 04:09 PM (14 years, 10 months ago)

Quote:

myceleus_rex said:
Quote:

Jiggle said:
Only because I find it easier to screw up a glc than to screw up a g2g. And in this situation where it's your last chance before you have to buy more spores I would just prefer to take the safest route.



You really think so? You don't even have to work with any open jars to do a GLC.

In either case, I'd wait, if possible, for full colonization.





I have no idea what OP's experience is but in my experience it's a lot harder to contaminate a master grain jar than an LC. Plus if your jar gets contaminated you can generally tell.

But I like the fact that theres a whole network of strong myc already in the jar that can fend off some contaminants where as in the LC there isn't.

Extras: Filter Print Post Top
OfflineDamion5050
Mush Doctor
Male User Gallery

Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation ! Flag
Last seen: 3 years, 11 months
Trusted Cultivator
Re: culture syringe from grain [Re: Jiggle]
    #12055038 - 02/18/10 04:10 PM (14 years, 10 months ago)

Yeah but a grain LC is just water and mycelium so there really is no way to get a contamination unless you introduce one by not flaming the needle or buy have an already contaminated grain jar.

Extras: Filter Print Post Top
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: culture syringe from grain [Re: Damion5050]
    #12055051 - 02/18/10 04:14 PM (14 years, 10 months ago)

Quote:

Damion5050 said:
Yeah but a grain LC is just water and mycelium so there really is no way to get a contamination unless you introduce one by not flaming the needle or buy have an already contaminated grain jar.



:thumbup:
tru dat
cause truskool says so


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
OfflineJiggle
Peanut Herder
Male


Registered: 01/13/10
Posts: 360
Loc: The South
Last seen: 11 years, 7 months
Re: culture syringe from grain [Re: truskool]
    #12055073 - 02/18/10 04:17 PM (14 years, 10 months ago)

Well, like I said it's just my opinion that g2g's are easier. If I was doing bulk with 40 or so jars I would do LC. For smaller scale cultivation though..I prefer G2G.

Extras: Filter Print Post Top
OfflineDamion5050
Mush Doctor
Male User Gallery

Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation ! Flag
Last seen: 3 years, 11 months
Trusted Cultivator
Re: culture syringe from grain [Re: truskool]
    #12055074 - 02/18/10 04:17 PM (14 years, 10 months ago)

LoL I am glad you approve of my bad grammar lol

Extras: Filter Print Post Top
OfflineDamion5050
Mush Doctor
Male User Gallery

Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation ! Flag
Last seen: 3 years, 11 months
Trusted Cultivator
Re: culture syringe from grain [Re: Jiggle]
    #12055082 - 02/18/10 04:19 PM (14 years, 10 months ago)

Quote:

Jiggle said:
Well, like I said it's just my opinion that g2g's are easier. If I was doing bulk with 40 or so jars I would do LC. For smaller scale cultivation though..I prefer G2G.




A smart cultivator IMO would do a grain jar take that to a grain LC then knock up 10 jars then use those 10 jars as masters and expand those 10 jars with G2G to 100 jars then once you use all those get your LC back out and make 10 more master jars.

Extras: Filter Print Post Top
OfflineJiggle
Peanut Herder
Male


Registered: 01/13/10
Posts: 360
Loc: The South
Last seen: 11 years, 7 months
Re: culture syringe from grain [Re: Damion5050]
    #12055212 - 02/18/10 04:39 PM (14 years, 10 months ago)

Quote:

Damion5050 said:
Quote:

Jiggle said:
Well, like I said it's just my opinion that g2g's are easier. If I was doing bulk with 40 or so jars I would do LC. For smaller scale cultivation though..I prefer G2G.




A smart cultivator IMO would do a grain jar take that to a grain LC then knock up 10 jars then use those 10 jars as masters and expand those 10 jars with G2G to 100 jars then once you use all those get your LC back out and make 10 more master jars.




A smart cultivator IMO would do g2g's with a colonised jar then when there is only 50% of the substrate left make a grain LC that way if your LC fails you have those other jars to fall back on, and since it's unlikely you will contaminate your grain transferring jar since colonized sub is very resistant to contaminations it's just an extra safe method IMO.

:2cents:

Extras: Filter Print Post Top
Invisiblemyceleus_rex
seeker
Male User Gallery


Registered: 07/01/09
Posts: 1,581
Loc: alized
Re: culture syringe from grain [Re: ce_greg]
    #12055431 - 02/18/10 05:14 PM (14 years, 10 months ago)

A GLC is a competely different animal from a standard sugar LC. With a sugar LC you are flying blind. You don't know if the culture is good until you try it out. With a GLC, you work from a healthy grain jar and make a myc slurry. You don't use it unless it looks like healthy mycelium.  "Culture" in the case of GLC is probably even a misnomer.  The only growing you do is in the originating grain jar. You grow healthy Myceleum, then strip it off into distilled water, which you can store in a sealed container for a far longer period than a sugar LC. Plus, like I said, you don't even need to open a jar in the whole process. It's actually less risky and far more productive than G2G in most situations.


--------------------
Cervantes' sclerotia thread    Citric's self-healing lid tek  Agar's Grain LC  Breaking Up Spawn Jars

World's Ugliest Cat?

If ignorance is bliss, why aren't there more happy people?

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Left Coast Kratom Buy Kratom Extract   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   Original Sensible Seeds Autoflowering Cannabis Seeds   Kraken Kratom Red Vein Kratom   Bridgetown Botanicals CBD Concentrates   North Spore Bulk Substrate   Mushroom-Hut Liquid Cultures   Myyco.com Isolated Cubensis Liquid Culture For Sale   MagicBag.co All-In-One Bags That Don't Suck   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* Help! Liquid culture on grain going bad wrong Submob 2,676 4 10/26/03 03:01 AM
by Submob
* Can I use rye grains to start honey h20? LucidDayDream 1,011 4 03/09/02 01:51 PM
by BeppoMarx
* PF Cake to Liquid culture transfer? Rohypnol 2,786 5 10/25/03 05:05 PM
by Hanky
* Never success with Monotubs - maybe bacteria contamination? apshroom 1,530 16 06/12/18 07:08 PM
by apshroom
* NEW Tissue Culture Technique - No agar, No flow hood, easy as hell
( 1 2 all )
funkyballoon 21,902 38 05/11/01 11:08 AM
by MAIA
* Liquid culture not starting up... Why?
( 1 2 3 4 5 all )
paranoidMushroomer 3,815 88 05/27/18 07:40 AM
by wushroow
* WET GRAINS! Anonymous 5,250 9 10/21/03 05:52 AM
by shirley knott
* Does your grain ever touch the filter when you shake? Atman Alaya 741 12 05/27/18 12:00 PM
by Atman Alaya

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
615 topic views. 10 members, 96 guests and 18 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.025 seconds spending 0.008 seconds on 12 queries.