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OfflineSrching4Reality
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PDA dishes Contam & sterilization
    #12048383 - 02/17/10 04:23 PM (14 years, 10 months ago)

-Posted in Contamination Forum but few people are viewing-

hey everyone; ok so I cooked up some PDA on Sunday, and allowed until today for the condensation to reabsorb (they are stacked). However, when I checked today, 5-9 dishes appeared contaminated (quickly took out of room) but I took some pictures and am curious as what kind of contams these are? I know the green one is trich. Also; how should I sterilize my dishes? open, scrape out, wash with soap and water? then PC? or PC, then wash, and PC when needed?




Edit: so pics are not that great quality - Sony A330 didn't want to focus for me.

15 views....kinda need a reply since I have the plates in tuperware (as not to spread spore or anything) waiting to be steralized (in w/e way is recommended here)

Edited by Srching4Reality (02/17/10 05:35 PM)

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InvisibleDoc_T
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Re: PDA dishes Contam & sterilization [Re: Srching4Reality]
    #12048994 - 02/17/10 05:40 PM (14 years, 10 months ago)

Safest would be to PC them as-is, then scrape out the stuff.
But I'd probably just run some water and flip the agar out into the disposal or a bag, then wash the dish.


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OfflineSrching4Reality
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Re: PDA dishes Contam & sterilization [Re: Doc_T]
    #12051923 - 02/18/10 03:13 AM (14 years, 10 months ago)

kk, cool thanks Doc

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InvisiblePsilliCoder
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Re: PDA dishes Contam & sterilization [Re: Srching4Reality]
    #12052141 - 02/18/10 05:57 AM (14 years, 10 months ago)

Yeah, pcing the dish first would be the smartest option here. I would also scrape out the dish outside on a porch or something if you could.


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OfflineRogerRabbitM
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Re: PDA dishes Contam & sterilization [Re: PsilliCoder]
    #12052454 - 02/18/10 07:45 AM (14 years, 10 months ago)

Just about everyone gets glass petri dishes the first time, and then quickly realize plastic pre-sterilized are the way to go.  I'm sure it will cost more in soap and energy to clean and re-sterilize glass dishes than the plastic ones cost.
RR


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OfflineSrching4Reality
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Re: PDA dishes Contam & sterilization [Re: RogerRabbit]
    #12053733 - 02/18/10 12:38 PM (14 years, 10 months ago)

ha Thanks RR; but they are soo0o0o much more scientific looking:eek: erm...gonna buy 100 plastic from mycosupply today  :headbang3: lol

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