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Phanodude
Sir
Registered: 01/18/10
Posts: 475
Loc: US of A
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Experienced Shroomers Cometh Hither, Forst I Have Ye Question
#12042965 - 02/16/10 08:34 PM (14 years, 10 months ago) |
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Lame Title, Now that we're over that these are the things that i have
Items: 1x Microscope 1x Spore Print (Acadian Coast or Texas) in a bag and folded over on foil 3x 12cc 18 Gauge Syringes 1x King size comforter set (The bag that holds it all with 2 holes in the side as a makeshift Glovebox) 1x Large Pot 1x7.5 Qt Pressure Cooker 1x Bag of Organic Rice Flour 1x Bag Of Vermiculite 1x Bag Of Pearlite 1x Roll Of Tin Foil 1x 91% Isopropyl Alcohol 1x Hydrogen Peroxide
My plan is to boil water, draw up boiling water, expell into sink, repeat 3 times, then pull up boiling water and cover syringe in foil, put it in freezer to cool momentarily. This is where my questions involving sterility and noobieness to the shroom game takes hold of my plans and prevents me from going further.
I'm pretty sure that's everything i've got/need. Now, the question that stands is, what's the best way i can ensure that the spores i have can get into a syringe with no contamination? Please don't reference TEK's (like don't just be like "Douchey Mcgee's Syringe Tek" with just a link) because i've read most of them but i'd like to get information from working sources. I'll do my best to respond within a timely manner.
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evildee125
Here now



Registered: 03/23/09
Posts: 3,179
Loc: fl
Last seen: 3 years, 3 months
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Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: Phanodude]
#12043027 - 02/16/10 08:43 PM (14 years, 10 months ago) |
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i put in boling water a ceramic espresso cup or shot glass, a knife or something to scrape the print off the foil and syringe.. i do with the syringe as you described but instead i place the cap and leave it in the water along with the cup and place the lid.. i close all windows and air vents allowing the air in my room to settle while the items cool.. i place the pot in the room and just wait like 15 minutes or so.. i take out the cup empty any water in it.. then scrape the print into the cup.. squirt contents of syringe into cup .. then draw the solution into the syringes.. hope that helps.. you could do this in a still air or glove box.. i make do without them.. keeping in mind always that the purpose of them being still air.. so doing these kinds of thing in a clean space for which the air has done some settling works out for me
actually the cup and knife are sterilized in the same pot.. i use a different pot for the syringe.. once i draw up the water i let them cool then do as described above
Edited by evildee125 (02/16/10 08:45 PM)
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xxxem3ryxxx
hi



Registered: 03/04/09
Posts: 325
Loc: Maryland
Last seen: 14 years, 5 months
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Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: Phanodude]
#12043060 - 02/16/10 08:46 PM (14 years, 10 months ago) |
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i wouldn't put it in the freezer, i let mine cool overnight out in the open. just make sure you sterilize everything with that alcohol. use the glovebox, i wear gloves then rub them with alc. rub the shot glass or watever with alc rag then let air dry, sterilize the tip of the syringe or whatever your scraping the spores in the glass with alc and lighter.
thats all i can think of....be sterile
-------------------- “I tried marijuana once. I did not inhale.” - Bill Clinton
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shangrila
member


Registered: 02/18/04
Posts: 623
Last seen: 1 year, 11 months
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Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: Phanodude]
#12043113 - 02/16/10 08:53 PM (14 years, 10 months ago) |
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.
Edited by shangrila (02/16/10 08:54 PM)
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evildee125
Here now



Registered: 03/23/09
Posts: 3,179
Loc: fl
Last seen: 3 years, 3 months
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Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: xxxem3ryxxx]
#12043128 - 02/16/10 08:55 PM (14 years, 10 months ago) |
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Quote:
xxxem3ryxxx said: i wouldn't put it in the freezer, i let mine cool overnight out in the open. just make sure you sterilize everything with that alcohol. use the glovebox, i wear gloves then rub them with alc. rub the shot glass or watever with alc rag then let air dry, sterilize the tip of the syringe or whatever your scraping the spores in the glass with alc and lighter.
thats all i can think of....be sterile
yes add all this to my previous post forgot to mention.. dont forget the gloves be clean be quick..sterilize the needle tip .. all as stated above
Edited by evildee125 (02/16/10 08:57 PM)
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Phanodude
Sir
Registered: 01/18/10
Posts: 475
Loc: US of A
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Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: evildee125]
#12043422 - 02/16/10 09:31 PM (14 years, 10 months ago) |
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Would it be safe enough to just alcohol sterilize my syringe tips? I don't want lil pieces of burnt residue throughout my syringe or is that an unrealistic worry?
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xxxem3ryxxx
hi



Registered: 03/04/09
Posts: 325
Loc: Maryland
Last seen: 14 years, 5 months
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Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: Phanodude]
#12043438 - 02/16/10 09:33 PM (14 years, 10 months ago) |
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Quote:
Phanodude said: Would it be safe enough to just alcohol sterilize my syringe tips? I don't want lil pieces of burnt residue throughout my syringe or is that an unrealistic worry?
flame sterilize. you dont have to worry about nothing
-------------------- “I tried marijuana once. I did not inhale.” - Bill Clinton
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biologys
Mycologist in Trainning



Registered: 12/21/09
Posts: 4,622
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Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: Phanodude]
#12043447 - 02/16/10 09:34 PM (14 years, 10 months ago) |
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they wnt hurt anything, buy an alcohol burner for 10 bucks and you dont get the black on your needle. i wipe my syringe with alcohol wipe after burning
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xxxem3ryxxx
hi



Registered: 03/04/09
Posts: 325
Loc: Maryland
Last seen: 14 years, 5 months
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Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: biologys]
#12043462 - 02/16/10 09:36 PM (14 years, 10 months ago) |
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Quote:
biologys said: they wnt hurt anything, buy an alcohol burner for 10 bucks and you dont get the black on your needle. i wipe my syringe with alcohol wipe after burning
hmm i wipe mine before flaming. both must work fine
-------------------- “I tried marijuana once. I did not inhale.” - Bill Clinton
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Phanodude
Sir
Registered: 01/18/10
Posts: 475
Loc: US of A
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Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: xxxem3ryxxx]
#12043525 - 02/16/10 09:44 PM (14 years, 10 months ago) |
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The print i have is about a 1 1/2- 2 inches around of print. How many syringes can i expect this to make? Also, said print is in on a piece of foil that has been folded over in half, then each side folded about a 1/4 inch to seal it. What's the best way for me to get at this badboy? Tips/tricks are welcome!
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truskool
WTF?? FTW!!



Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
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Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: Phanodude]
#12043676 - 02/16/10 10:05 PM (14 years, 10 months ago) |
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I use a size of this -------> O to make 10ml of syringe. I pretty much unfld yet not oipen the print b4 putting in the GB
-------------------- Ask AMU for the best mycology advice out there
Roll it While I troll it.
I don't mean to boast, but dam if I don't brag.
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Phanodude
Sir
Registered: 01/18/10
Posts: 475
Loc: US of A
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Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: truskool]
#12044841 - 02/17/10 03:07 AM (14 years, 10 months ago) |
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Update: I just finished making them. Process:
Sucked up boiling water and squirted out a few times (think i forgot to with one syringe which sucks because if that contams the whole batch does, the water was mixed) and then sterilized a knife. I ran water over the print to loosen up spores and ran it into the glass, then i scraped it off and it made a purple mud so i ran s'more water over it (i retrieved from the glass) and got a nice solution. I've now got 18 cc's of either acadian coast or texas.
If it contams i'm gonna buy a syringe but i think we'll be ok.
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passenger
Stranger


Registered: 11/05/08
Posts: 337
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Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: Phanodude]
#12044879 - 02/17/10 03:33 AM (14 years, 10 months ago) |
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when flaming the syringe there is no need to wipe it either before or after you flame. the water you shoot out of it into you're shot glass will cool the tip.
to get spores off you're print just shoot about 7/8th or so of you're sterile water from you're syringe (after you flamed it) into the shot glass then put a tiny drop on the foil next to where you want to take from. then use a innoc loop or sterile knife or something similiar and work the drop of water into the print just to loosen up a few spores. you DON'T want a slurry of spores. take you're syringe with a little bit of water left in it in one hand and in the other the print with the spore solution. hold over the shot glass and rinse the solution into the glass. suck it up once and shoot it back into the shot glass to get it all mixed up and you're done. simple.
EDIT. don't use that syringe that you we're unsure about.
Edited by passenger (02/17/10 03:34 AM)
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Phanodude
Sir
Registered: 01/18/10
Posts: 475
Loc: US of A
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Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: passenger]
#12048365 - 02/17/10 04:21 PM (14 years, 10 months ago) |
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That's gonna be a task because i mixed the two up in the GB and don't know which is which 
Either way it's a learning experience and i only inoculated 4 WBS jars so if they contam they contam and i'll get a syringe from a vendor and refine my technique at a later date.
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