Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Capsules   MagicBag.co Certified Organic All-In-One Grow Bags by Magic Bag   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   Myyco.com Golden Teacher Liquid Culture For Sale   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisiblePhanodude
Sir
Registered: 01/18/10
Posts: 475
Loc: US of A Flag
Experienced Shroomers Cometh Hither, Forst I Have Ye Question
    #12042965 - 02/16/10 08:34 PM (14 years, 10 months ago)

Lame Title, Now that we're over that these are the things that i have

Items:
1x Microscope
1x Spore Print (Acadian Coast or Texas) in a bag and folded over on foil
3x 12cc 18 Gauge Syringes
1x King size comforter set (The bag that holds it all with 2 holes in the side as a makeshift Glovebox)
1x Large Pot
1x7.5 Qt Pressure Cooker
1x Bag of Organic Rice Flour
1x Bag Of Vermiculite
1x Bag Of Pearlite
1x Roll Of Tin Foil
1x 91% Isopropyl Alcohol
1x Hydrogen Peroxide

My plan is to boil water, draw up boiling water, expell into sink, repeat 3 times, then pull up boiling water and cover syringe in foil, put it in freezer to cool momentarily. This is where my questions involving sterility and noobieness to the shroom game takes hold of my plans and prevents me from going further.

I'm pretty sure that's everything i've got/need. Now, the question that stands is, what's the best way i can ensure that the spores i have can get into a syringe with no contamination? Please don't reference TEK's (like don't just be like "Douchey Mcgee's Syringe Tek" with just a link) because i've read most of them but i'd like to get information from working sources. I'll do my best to respond within a timely manner.

Extras: Filter Print Post Top
Offlineevildee125
Here now
Male User Gallery


Registered: 03/23/09
Posts: 3,179
Loc: fl
Last seen: 3 years, 3 months
Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: Phanodude]
    #12043027 - 02/16/10 08:43 PM (14 years, 10 months ago)

i put in boling water a ceramic espresso cup or shot glass, a knife or something to scrape the print off the foil and syringe.. i do with the syringe as you described but instead i place the cap and leave it in the water along with the cup and place the lid.. i close all windows and air vents allowing the air in my room to settle while the items cool.. i place the pot in the room and just wait like 15 minutes or so.. i take out the cup empty any water in it.. then scrape the print into the cup.. squirt contents of syringe into cup .. then draw the solution into the syringes.. hope that helps.. you could do this in a still air or glove box.. i make do without them..  keeping in mind always that the purpose of them being still air.. so doing these kinds of thing in a clean space for which the air has done some settling works out for me

actually the cup and knife are sterilized in the same pot.. i use a different pot for the syringe.. once i draw up the water i let them cool then do as described above


--------------------
http://www.shroomery.org/forums/banlist.php http://www.shroomery.org/forums/banomatic.php

http://www.shroomery.org/forums/vetstatus.php

Kaleidoscope said:
If you build an idiot-proof device, someone will build a better idiot.

Edited by evildee125 (02/16/10 08:45 PM)

Extras: Filter Print Post Top
Offlinexxxem3ryxxx
hi
Male


Registered: 03/04/09
Posts: 325
Loc: Maryland Flag
Last seen: 14 years, 5 months
Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: Phanodude]
    #12043060 - 02/16/10 08:46 PM (14 years, 10 months ago)

i wouldn't put it in the freezer, i let mine cool overnight out in the open. just make sure you sterilize everything with that alcohol. use the glovebox, i wear gloves then rub them with alc. rub the shot glass or watever with alc rag then let air dry,  sterilize the tip of the syringe or whatever your scraping the spores in the glass with alc and lighter.

thats all i can think of....be sterile


--------------------
“I tried marijuana once. I did not inhale.” - Bill Clinton

Extras: Filter Print Post Top
Offlineshangrila
member
 User Gallery

Registered: 02/18/04
Posts: 623
Last seen: 1 year, 11 months
Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: Phanodude]
    #12043113 - 02/16/10 08:53 PM (14 years, 10 months ago)

.

Edited by shangrila (02/16/10 08:54 PM)

Extras: Filter Print Post Top
Offlineevildee125
Here now
Male User Gallery


Registered: 03/23/09
Posts: 3,179
Loc: fl
Last seen: 3 years, 3 months
Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: xxxem3ryxxx]
    #12043128 - 02/16/10 08:55 PM (14 years, 10 months ago)

Quote:

xxxem3ryxxx said:
i wouldn't put it in the freezer, i let mine cool overnight out in the open. just make sure you sterilize everything with that alcohol. use the glovebox, i wear gloves then rub them with alc. rub the shot glass or watever with alc rag then let air dry,  sterilize the tip of the syringe or whatever your scraping the spores in the glass with alc and lighter.

thats all i can think of....be sterile




yes add all this to my previous post forgot to mention.. dont forget the gloves be clean be quick..sterilize the needle tip .. all as stated above


--------------------
http://www.shroomery.org/forums/banlist.php http://www.shroomery.org/forums/banomatic.php

http://www.shroomery.org/forums/vetstatus.php

Kaleidoscope said:
If you build an idiot-proof device, someone will build a better idiot.

Edited by evildee125 (02/16/10 08:57 PM)

Extras: Filter Print Post Top
InvisiblePhanodude
Sir
Registered: 01/18/10
Posts: 475
Loc: US of A Flag
Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: evildee125]
    #12043422 - 02/16/10 09:31 PM (14 years, 10 months ago)

Would it be safe enough to just alcohol sterilize my syringe tips? I don't want lil pieces of burnt residue throughout my syringe or is that an unrealistic worry?

Extras: Filter Print Post Top
Offlinexxxem3ryxxx
hi
Male


Registered: 03/04/09
Posts: 325
Loc: Maryland Flag
Last seen: 14 years, 5 months
Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: Phanodude]
    #12043438 - 02/16/10 09:33 PM (14 years, 10 months ago)

Quote:

Phanodude said:
Would it be safe enough to just alcohol sterilize my syringe tips? I don't want lil pieces of burnt residue throughout my syringe or is that an unrealistic worry?




flame sterilize. you dont have to worry about nothing


--------------------
“I tried marijuana once. I did not inhale.” - Bill Clinton

Extras: Filter Print Post Top
Invisiblebiologys
Mycologist in Trainning
Male

Folding@home Statistics
Registered: 12/21/09
Posts: 4,622
Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: Phanodude]
    #12043447 - 02/16/10 09:34 PM (14 years, 10 months ago)

they wnt hurt anything, buy an alcohol burner for 10 bucks and you dont get the black on your needle. i wipe my syringe with alcohol wipe after burning

Extras: Filter Print Post Top
Offlinexxxem3ryxxx
hi
Male


Registered: 03/04/09
Posts: 325
Loc: Maryland Flag
Last seen: 14 years, 5 months
Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: biologys]
    #12043462 - 02/16/10 09:36 PM (14 years, 10 months ago)

Quote:

biologys said:
they wnt hurt anything, buy an alcohol burner for 10 bucks and you dont get the black on your needle. i wipe my syringe with alcohol wipe after burning




hmm i wipe mine before flaming. both must work fine:grin:


--------------------
“I tried marijuana once. I did not inhale.” - Bill Clinton

Extras: Filter Print Post Top
InvisiblePhanodude
Sir
Registered: 01/18/10
Posts: 475
Loc: US of A Flag
Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: xxxem3ryxxx]
    #12043525 - 02/16/10 09:44 PM (14 years, 10 months ago)

The print i have is about a 1 1/2- 2 inches around of print. How many syringes can i expect this to make? Also, said print is in on a piece of foil that has been folded over in half, then each side folded about a 1/4 inch to seal it. What's the best way for me to get at this badboy? Tips/tricks are welcome!

Extras: Filter Print Post Top
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: Phanodude]
    #12043676 - 02/16/10 10:05 PM (14 years, 10 months ago)

I use a size of this -------> O to make 10ml of syringe.  I pretty much unfld yet not oipen the print b4 putting in the GB


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
InvisiblePhanodude
Sir
Registered: 01/18/10
Posts: 475
Loc: US of A Flag
Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: truskool]
    #12044841 - 02/17/10 03:07 AM (14 years, 10 months ago)

Update: I just finished making them. Process:

Sucked up boiling water and squirted out a few times (think i forgot to with one syringe which sucks because if that contams the whole batch does, the water was mixed) and then sterilized a knife. I ran water over the print to loosen up spores and ran it into the glass, then i scraped it off and it made a purple mud so i ran s'more water over it (i retrieved from the glass) and got a nice solution. I've now got 18 cc's of either acadian coast or texas.

If it contams i'm gonna buy a syringe but i think we'll be ok.

Extras: Filter Print Post Top
Invisiblepassenger
Stranger


Registered: 11/05/08
Posts: 337
Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: Phanodude]
    #12044879 - 02/17/10 03:33 AM (14 years, 10 months ago)

when flaming the syringe there is no need to wipe it either before or after you flame. the water you shoot out of it into you're shot glass will cool the tip.

to get spores off you're print just shoot about 7/8th or so of you're sterile water from you're syringe (after you flamed it) into the shot glass then put a tiny drop on the foil next to where you want to take from. then use a innoc loop or sterile knife or something similiar and work the drop of water into the print just to loosen up a few spores. you DON'T want a slurry of spores. take you're syringe with a little bit of water left in it in one hand and in the other the print with the spore solution. hold over the shot glass and rinse the solution into the glass. suck it up once and shoot it back into the shot glass to get it all mixed up and you're done. simple.


EDIT. don't use that syringe that you we're unsure about.

Edited by passenger (02/17/10 03:34 AM)

Extras: Filter Print Post Top
InvisiblePhanodude
Sir
Registered: 01/18/10
Posts: 475
Loc: US of A Flag
Re: Experienced Shroomers Cometh Hither, Forst I Have Ye Question [Re: passenger]
    #12048365 - 02/17/10 04:21 PM (14 years, 10 months ago)

That's gonna be a task because i mixed the two up in the GB and don't know which is which :facepalm:

Either way it's a learning experience and i only inoculated 4 WBS jars so if they contam they contam and i'll get a syringe from a vendor and refine my technique at a later date.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Injection Grain Bag   Left Coast Kratom Buy Kratom Capsules   MagicBag.co Certified Organic All-In-One Grow Bags by Magic Bag   Kraken Kratom Red Vein Kratom   Mushroom-Hut Liquid Cultures   Myyco.com Golden Teacher Liquid Culture For Sale   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* new grower. simple question. whiterastahippie 1,022 4 08/20/02 05:32 PM
by daussaulit
* Sorry for mking U read this (harvest questions) paranoiac21 2,454 7 08/23/01 11:32 AM
by puscle
* Quick Casing Question Redstorm 873 5 02/15/03 06:37 AM
by Alger
* Another Stupid NooB Askin Questions SlamDog_C 1,008 2 01/17/02 01:38 PM
by XAZIA
* Re: PF TEK Question Gulf_Shroomer 584 2 04/29/01 05:19 AM
by ISaToriBluEI
* What tek's do you experienced growers use? Shroom1119 2,140 4 04/22/02 10:14 AM
by ParticleMan
* Hello everyone! I have some questions... Angry Mycologist 708 3 11/24/02 11:45 AM
by Dogomush
* Newbie with some questions... Cusero 1,275 7 07/15/02 06:25 AM
by Cusero

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
582 topic views. 10 members, 78 guests and 15 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.007 seconds on 12 queries.