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OfflineTony
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Registered: 09/25/09
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Immortal mycelium in a LC - only an injection port needed
    #12038639 - 02/16/10 08:12 AM (14 years, 10 months ago)

This is just an idea I had today. I'm posting it here because I'd like to know if anyone has tried a similar method. It's written in a matter-of-fact tone, but everything is purely theoretical.

Like most of you know the problem with having no filter disc on a LC jar is that when you draw solution into a syringe, a negative pressure forms within the jar which draws in air from the outside. Since there is no filter to clean that air, it is a potential vector for contaminants.

One solution (here comes the theory) is to use a syringe with sterilized nutrient solution already in it. You insert the needle through the port as you normally would, but instead of simply drawing LC in to fill an empty syringe, you expell some of the solution inside the syringe into the LC container. This creates pressure inside the jar, which allows you to draw the same amount of solution back into the syringe without causing negative pressure and air-intake.

After this pumping action is repeated a few times, there should be enough mycelium in the syringe to promote growth. You simply incubate it for a while and then it's ready to use or store in the fridge.

The point is that the solution's volume and nutrient content stay roughly the same over time and, in the meanwhile, air-intake and contaminations are minimized. In theory the same LC could be used again and again indefinitely. (And, to avoid senescence, new genetic material could always be introduced via a syringe. Edit: On second thought, I dunno if an already-dikaryotic mycelium strain can exchange gentetic material with other dikaryotic strains without going through spore germination and plasmogamy. I guess senescense would eventually become a problem. But that's just a side-point.)

You can also periodically change the type of nutrient solution you supply the mycelium with to keep it from getting used to a specific ratio of nutrients.

So that's the idea. Please share your thoughts :smile:

Edited by Tony (02/16/10 08:29 AM)

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Offlinescifi5001
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Re: Immortal mycelium in a LC - only an injection port needed [Re: Tony]
    #12038672 - 02/16/10 08:29 AM (14 years, 10 months ago)

Or you could just draw air into the syringe with the needle above a flame, pump the air into the jar and draw solution?


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Nothing lasts, nothing is lost.

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InvisibleDoc_T
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Re: Immortal mycelium in a LC - only an injection port needed [Re: Tony]
    #12038691 - 02/16/10 08:35 AM (14 years, 10 months ago)

Contam city.

You don't inject anything into a good LC- you draw off portions and store them safely and separately.
Then use those as masters to grow out more clean LC.


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You make it all possible. Doesn't it feel good?

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Offlinejim617
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Re: Immortal mycelium in a LC - only an injection port needed [Re: Tony]
    #12038733 - 02/16/10 08:46 AM (14 years, 10 months ago)

Try it out, all you need is water in a jar to test the theory

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OfflineTony
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Re: Immortal mycelium in a LC - only an injection port needed [Re: jim617]
    #12038757 - 02/16/10 08:54 AM (14 years, 10 months ago)

I think I need to test the theory with a proper LC and then make cakes with it. That's the only way to be sure. Probably someone else has already tried something similar though. At least there has to be someone who injects nute solution into near-depleted LCs to keep them going?

Or, like Doc_T said, maybe you just simply don't inject anything into a good LC.

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InvisibleDoc_T
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Re: Immortal mycelium in a LC - only an injection port needed [Re: Tony]
    #12038834 - 02/16/10 09:16 AM (14 years, 10 months ago)

It's worth trying. It'll only cost you a few cents to answer the question.


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You make it all possible. Doesn't it feel good?

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Offlinedeliciosa
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Re: Immortal mycelium in a LC - only an injection port needed [Re: Tony]
    #12038854 - 02/16/10 09:22 AM (14 years, 10 months ago)

It sounds to me like it just may work as long as the syringe you are using has been properly sterilized.

I may give this a try when I get going here shortly.

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OfflineKysmoker
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Re: Immortal mycelium in a LC - only an injection port needed [Re: deliciosa]
    #12038864 - 02/16/10 09:25 AM (14 years, 10 months ago)

It does sound cool in theory. Hard saying if itll work though *shrug*


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Your class, your caste, your country, sect, your name or your tribe
There's people always dying trying to keep them alive
There are bodies decomposing in containers tonight
In an abandoned building where
A squatter's made a mural of a Mexican girl
With fifteen cans of spray paint in a chemical swirl
She's standing in the ashes at the end of the world
Four winds blowing through her hair

-Four Winds by Bright Eyes

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OfflineWimy
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Re: Immortal mycelium in a LC - only an injection port needed [Re: Kysmoker]
    #12039009 - 02/16/10 10:00 AM (14 years, 10 months ago)

air will bubble up around a silicone port if you inject into a full container

because i took a vial of sterile water and a heavy 2 cc spore solution and injected it in there. works for a wipe clean but you are still displacing air!


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OfflineTony
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Re: Immortal mycelium in a LC - only an injection port needed [Re: Wimy]
    #12039101 - 02/16/10 10:18 AM (14 years, 10 months ago)

Quote:

weiliiinmyyard said:
air will bubble up around a silicone port if you inject into a full container




That's the problem I'm anticipating. My only hope is that there is a reasonable treshold value above which air starts to bubble up. If the treshold is, say 0.5cc, then I could theoretically quickly inject about 0.4 cc in and out without air displacement. Also, if I wait for a while after pushing the needle through, the silicone might heal around the needle enough to allow for some pressure variation during the pumping. Hmm...

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OfflineWimy
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Re: Immortal mycelium in a LC - only an injection port needed [Re: Tony]
    #12039182 - 02/16/10 10:33 AM (14 years, 10 months ago)

idk..

i'm with doc. the needle is gonna get you if youre new solution doesnt


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OfflinePrimeTimeSlime
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Re: Immortal mycelium in a LC - only an injection port needed [Re: Tony]
    #12039208 - 02/16/10 10:38 AM (14 years, 10 months ago)

That's a cool idea. Sounds great in theory but I guess you would have to try it out to see if it works.


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Offlinexbrutalx233
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Re: Immortal mycelium in a LC - only an injection port needed [Re: PrimeTimeSlime]
    #12039247 - 02/16/10 10:49 AM (14 years, 10 months ago)

I used to inject air into my LC's by sterilizing a syringe for of air in the pc and then injecting it in... worked fine for me...  what you should do though is get some tyvek and silicone a little tyvek breathe port...  that's worked well for me, and it doesn't leak or anything.


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:mushroom2:


I do not grow anything that is illegal... Everything is of the legal variety... if someone mentions any illegal activity it isn't me...

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Offlinebw86
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Re: Immortal mycelium in a LC - only an injection port needed [Re: xbrutalx233]
    #12039334 - 02/16/10 11:15 AM (14 years, 10 months ago)

have two injection ports
one you shove a needle with polyfil in
and the other you take out what you need.
thats what people have been doing for ever.

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Invisiblebadman
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Re: Immortal mycelium in a LC - only an injection port needed [Re: bw86]
    #12039380 - 02/16/10 11:28 AM (14 years, 10 months ago)

Quote:

bw86 said:
have two injection ports
one you shove a needle with polyfil in
and the other you take out what you need.
thats what people have been doing for ever.




:superiority:

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OfflineWimy
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Re: Immortal mycelium in a LC - only an injection port needed [Re: badman]
    #12040397 - 02/16/10 02:37 PM (14 years, 10 months ago)

can you elaborate on "take a needle with polyfil"

like a syringe filled with polyfil and two silicone injection ports??

haha..


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OfflineMycjunky
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Re: Immortal mycelium in a LC - only an injection port needed [Re: bw86]
    #12040528 - 02/16/10 03:01 PM (14 years, 10 months ago)

Quote:

bw86 said:
have two injection ports
one you shove a needle with polyfil in
and the other you take out what you need.
thats what people have been doing for ever.




Exactly what I do. Just ball up some polyfill and stuff it into the opening of the needle were you'd normally attach the syringe. Then you have a filtered port and can suck as much solution up as possible. No reason to really inject more solution, you should look into grain lcs which provide an endless supply of lc and you can be sure it's not contamed.

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OfflineSeniorCoolo
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Re: Immortal mycelium in a LC - only an injection port needed [Re: Wimy]
    #12040573 - 02/16/10 03:11 PM (14 years, 10 months ago)

Quote:

weiliiinmyyard said:
can you elaborate on "take a needle with polyfil"

like a syringe filled with polyfil and two silicone injection ports??

haha..





Have a lid with 2 injection ports. Take a syringe with a needle. Take the plunger completely out. Put some polyfil in the syringe (I put some dry polyfil in, then some soaked in rubbing alcohol on top of that). Put the polyfil syringe in one injection port. Suck up however much LC you need from port #2. Remove both needles. Viola! You have a LC syringe.

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OfflineWimy
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Re: Immortal mycelium in a LC - only an injection port needed [Re: SeniorCoolo]
    #12040725 - 02/16/10 03:32 PM (14 years, 10 months ago)

haha clever


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OfflineKillaFoRilla
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Re: Immortal mycelium in a LC - only an injection port needed [Re: SeniorCoolo]
    #12040732 - 02/16/10 03:33 PM (14 years, 10 months ago)

I would like to see some data before I try this.  I can't see injecting anything into a perfect .5qt of LC. I can only see taking it out to spawn or make more LC.

Every time you stick your jar with a needle, you have the possibility of introducing contams. Why up the odds??


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