Home | Community | Message Board

Magic-Mushrooms-Shop.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals CBD Concentrates   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds High THC Strains   PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinejdominate
Stranger

Registered: 07/13/09
Posts: 24
Last seen: 13 years, 4 months
Strain degeneration question
    #12036471 - 02/15/10 08:15 PM (14 years, 10 months ago)

I only had 1 Syringe of my original PE 1 GLC culture left so i decided to replicate it and make some more...

Here is a chart showing the complete timeline of the strain.



So there shouldent be any noticable difference in the performance of the PE 1.1 and 1.2 replicate in comparison to the original PE 1 culture, right?

In theory since GLC this is basically the same as GTG, from what i've read there shouldent be any noticable difference till you get past the 3rd generation.

I think im in the right ball park. Can anyone help me understand this.

Extras: Filter Print Post Top
Offlinejdominate
Stranger

Registered: 07/13/09
Posts: 24
Last seen: 13 years, 4 months
Re: Strain degeneration question [Re: jdominate]
    #12036585 - 02/15/10 08:37 PM (14 years, 10 months ago)

Ahh, here's the quote i was looking for:

RogerRabbit:
Quote:

*You can store G-1 LC in 5, 50 or 100 vials of sterile water (years & years).Any time you want G-1 pull a tube out of the Fridge & go for it. Either start a new LC with it, or go to grains, then G2G. STORING LC




So he's saying you can store generation 1 LC in the fridge, then knock up a grain jar and go GTG... Which is the exact same as knocking up a grain jar and making more GLC.

I guess i answered my own question.

Extras: Filter Print Post Top
Invisibletruskool
WTF?? FTW!!
Male User Gallery


Registered: 06/28/09
Posts: 11,194
Loc: Over the rainbow
Re: Strain degeneration question [Re: jdominate]
    #12036616 - 02/15/10 08:42 PM (14 years, 10 months ago)

Degeneration happens after so many cell expansions.  Ppl have went 4-5 generations without senseance


--------------------
Ask  AMU    for    the  best    mycology    advice  out  there

  Roll it :burnone:While I troll it.  :maximumtrolling:

I don't mean to boast, but dam if I don't brag.

Extras: Filter Print Post Top
Offlinejdominate
Stranger

Registered: 07/13/09
Posts: 24
Last seen: 13 years, 4 months
Re: Strain degeneration question [Re: truskool]
    #12039783 - 02/16/10 12:46 PM (14 years, 10 months ago)

Whoops after a little more reading i realize im backwards on this.

What im worried about is senescence, not strain degeneration.

Senescence occurs after too many gtg, glc, transfers.

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Bridgetown Botanicals CBD Concentrates   Myyco.com Isolated Cubensis Liquid Culture For Sale   Original Sensible Seeds High THC Strains   PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag


Similar ThreadsPosterViewsRepliesLast post
* Strain/Rice question Romulusguy 616 2 05/21/02 02:42 AM
by angryshroom
* Outdoor Strain Temp Question CaliGrower 831 2 11/27/02 07:39 PM
by CaliGrower
* Strain - High Question Pulseczar 3,019 15 02/11/02 06:27 PM
by queerbandit
* Wheat Straw Juice Questions? strain 1,556 6 02/27/02 08:07 PM
by strain
* P Strain Not pinning strain 3,512 6 03/25/02 08:23 AM
by GaNjAShRooM
* What to do with these P strain cakes/casings? strain 2,471 1 04/20/02 04:08 PM
by World Spirit
* Multi Spore Grain to Grain & Senescence Crabmeat 5,713 3 07/12/08 08:51 PM
by Bubba McMushies
* Mushroom Cultivation Categories of Questions: kykeon 2,804 9 11/19/02 08:50 PM
by fredthetree

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
363 topic views. 9 members, 67 guests and 14 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.017 seconds spending 0.006 seconds on 12 queries.