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Invisiblegrimeyobject
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BRF sterilization improvement question
    #12014321 - 02/12/10 06:54 AM (14 years, 11 months ago)

If you were to make a jar up ready for sterilization, then let it sit overnight with duct tape on the holes to allow any spore germination in the substrate to occur, then sterilized and went on with your business, would this improve contam rates?

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OfflineLucas89
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Re: BRF sterilization improvement question [Re: grimeyobject]
    #12014335 - 02/12/10 07:02 AM (14 years, 11 months ago)

that should be fine, ive made up brf cakes 24h before boiling them, with no problems at all.


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Offlinesolumvita
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Re: BRF sterilization improvement question [Re: Lucas89]
    #12015269 - 02/12/10 11:47 AM (14 years, 11 months ago)

yes it will help, but if the substrate is too wet you may start some anaerobic bacteria.


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Invisiblefastfred
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Re: BRF sterilization improvement question [Re: solumvita]
    #12015406 - 02/12/10 12:18 PM (14 years, 11 months ago)

That isn't going to do anything.  BRF should have no endospores to begin with.  That's only a problem with rye and other similar grains.

30 min at 15 psi should give you +99%, so there's no room for any improvement.


-FF


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OfflineRogerRabbitM
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Re: BRF sterilization improvement question (moved) [Re: grimeyobject]
    #12045413 - 02/17/10 08:47 AM (14 years, 10 months ago)

This thread was moved from Advanced Mycology.

Reason:
Off topic in advanced mycology.

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