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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Quote:
noobieshroomie said: that was after i washed my hands too just goes to show you why its good to wear gloves when doing agar work
-noobie-
Yep its true. After seeing your nail beds under a microscope after washing them you will want to ware gloves
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 21 days
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Re: Trich question [Re: Shea25]
#11979630 - 02/07/10 01:26 AM (14 years, 12 days ago) |
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Shea25 said:
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Kysmoker said: Since this questions been semi-answered, mind if I ask one? Its about trich and I dont feel like making another post.
I have one jar out of 45 that has shown a small green/bluish dot about 2cm in diameter. I took it away from the rest of my jars because im about 99% sure thats a contam. Is their any harm in letting it grow though? I wana see how this contam works, how quick it is, just study it a bit so I understand how they act a bit more.
Is this risky? Its still inside the jar, sealed up with a dry verm barrier. Its at the innoc point, so apparently it somehow got in when I inoculated (though its near the bottom of the jar).
Im just glad its one jar and not more, atleast for now >.>
P.S sorry for the thread jack, if you want me to delete my post just ask.
If you really want to study it then sure go a head its no prob. One tip thoe elastic band a coffee filter or 2 over the top of the jar. It will be contained and safe to study 
No way. Toss it. Trying to stop contaminant spores with coffee filters is like trying to stop a mosquito with a chain link fence. Even with the best synthetic filter disks, I've accidentally missed contaminated jars, and all the surrounding jars come down with trich too. Filters might stop 99.99% of the contaminant spores, but .001% of a few billion is a lot of spores to have floating around. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Quote:
RogerRabbit said:
Quote:
Shea25 said:
Quote:
Kysmoker said: Since this questions been semi-answered, mind if I ask one? Its about trich and I dont feel like making another post.
I have one jar out of 45 that has shown a small green/bluish dot about 2cm in diameter. I took it away from the rest of my jars because im about 99% sure thats a contam. Is their any harm in letting it grow though? I wana see how this contam works, how quick it is, just study it a bit so I understand how they act a bit more.
Is this risky? Its still inside the jar, sealed up with a dry verm barrier. Its at the innoc point, so apparently it somehow got in when I inoculated (though its near the bottom of the jar).
Im just glad its one jar and not more, atleast for now >.>
P.S sorry for the thread jack, if you want me to delete my post just ask.
If you really want to study it then sure go a head its no prob. One tip thoe elastic band a coffee filter or 2 over the top of the jar. It will be contained and safe to study 
No way. Toss it. Trying to stop contaminant spores with coffee filters is like trying to stop a mosquito with a chain link fence. Even with the best synthetic filter disks, I've accidentally missed contaminated jars, and all the surrounding jars come down with trich too. Filters might stop 99.99% of the contaminant spores, but .001% of a few billion is a lot of spores to have floating around. RR
No offense but I have had my fair share of contaminated jars in my grow areas. Most with filtered lids and or a dry verm barrier. I have left agar dishes out in the open and have fanned them into the open air into my grow area,I still maintain a low contam rate and mold spore count. I can see the fact that having a contamed vessel in the grow area can be bad if not contained right. But to want to study it is a viable option. I have seen no proof that keeping a jar around for a few extra days will greatly impact your chances of contams if one wants to study it in a safe manner Filtered/Sealed off. This is just based off my circumstances.
Thoe coffee filters are not the best option, when I first started to make lids with them for LC's I had a near flawless success ratio. Tyvek is much better suited thats for sure
Im not one to advocate keeping moldy or contamed shit around the house. But if you want to study it, keep it in a sealed container, that is what I should have said from the start. I would use coffee filter's or tyvek and then put it in a baggie as well.
Not many people want to study contams and I can see why. But doing so can be beneficial in a safe manner. Personally I dont keep contamed things around long anymore. I have had my experiences with them
Quote:
Filters might stop 99.99% of the contaminant spores, but .001% of a few billion is a lot of spores to have floating around.
True there are also billions when you open your door to get into your house. or open a window or turn on the heat in your house
Bottom line is that we should be accepting if people want to study contams its a good thing to know and study as long as its in a safe manner. Personally having a contamed jar here and there as long as there is a filtered and contained has not been detrimental to my grow area based off test's done with agar,
Opening a contamed jar and observing one in a filtered/sealed are two different scenarios.
As for petri dishes if one ones to study that way micropore tape around the dish and seal it off in a plastic baggie
Im not really looking for an argument I just think its perfectily fine for people to want to observe a contam. As long as its is in a safe manner .
Theres no need to discourage people from learning about contams look at noobieshroom he has some sweet pictures/pictures showing how dirty hands can be even after being washed. Should we scrutinize him for even having those plates around ? No it can be helpful.
Key words SEAL off the contam'd plate/jar something I should have been more clear on if you want to study it
Edited by Shea25 (02/07/10 02:25 AM)
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Kysmoker
Shroomer In Training



Registered: 01/05/10
Posts: 804
Last seen: 11 years, 3 months
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Quote:
RogerRabbit said:
Quote:
Shea25 said:
Quote:
Kysmoker said: Since this questions been semi-answered, mind if I ask one? Its about trich and I dont feel like making another post.
I have one jar out of 45 that has shown a small green/bluish dot about 2cm in diameter. I took it away from the rest of my jars because im about 99% sure thats a contam. Is their any harm in letting it grow though? I wana see how this contam works, how quick it is, just study it a bit so I understand how they act a bit more.
Is this risky? Its still inside the jar, sealed up with a dry verm barrier. Its at the innoc point, so apparently it somehow got in when I inoculated (though its near the bottom of the jar).
Im just glad its one jar and not more, atleast for now >.>
P.S sorry for the thread jack, if you want me to delete my post just ask.
If you really want to study it then sure go a head its no prob. One tip thoe elastic band a coffee filter or 2 over the top of the jar. It will be contained and safe to study 
No way. Toss it. Trying to stop contaminant spores with coffee filters is like trying to stop a mosquito with a chain link fence. Even with the best synthetic filter disks, I've accidentally missed contaminated jars, and all the surrounding jars come down with trich too. Filters might stop 99.99% of the contaminant spores, but .001% of a few billion is a lot of spores to have floating around. RR
If it gets any bigger or shows progression I will, right now its just stayed that small spot for 24 hours or so. Really confusing me =P
EDIT: I covered it with foil ducktaped on about 30 minutes ago. If it gets through that ill be impressed.
-------------------- Your class, your caste, your country, sect, your name or your tribe There's people always dying trying to keep them alive There are bodies decomposing in containers tonight In an abandoned building where A squatter's made a mural of a Mexican girl With fifteen cans of spray paint in a chemical swirl She's standing in the ashes at the end of the world Four winds blowing through her hair -Four Winds by Bright Eyes
Edited by Kysmoker (02/07/10 01:37 AM)
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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Re: Trich question [Re: Kysmoker]
#11979714 - 02/07/10 01:50 AM (14 years, 12 days ago) |
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Yea what I should have said said is use coffee filters and seal it off
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