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Amazon Shop for: ½ Pint Jars, Rye Grain

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OfflineMitchnast
Trial by Madness
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Registered: 10/28/99
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Loc: Okanagan
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will it contam?
    #1195804 - 01/06/03 08:10 PM (13 years, 11 months ago)

i have 4 sterilized jars (via pc)of rye berries, 2 litres each. i MEANT to inject spores in the oven tek. but realized i couldnt get the lids off at all. i have small holes in the lids and a tyvex seal. on that its aluminum foil. i had to puncture the tyvex (near the edge of the jars) to get the spores in. i quickly returned the foil and put them someplace warm and relitively clean (on top of fridge in new glad bags (not sealed) and all under a clean towel to maintain temp (fridge acts as incubator)
im worried the tyvex being broken will pretty much guarentee contams.
is this doomed? or is there a good chance i will have success, i used anti bacterial soap all over and did this quickly and sterilized my needle between each jar.
these jars are big enough to make a decent master if at leas one works. what are the odds based on this would you think? (anyone)


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Offlinesocratesmind
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Registered: 02/22/02
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Re: will it contam? [Re: Mitchnast]
    #1195811 - 01/06/03 08:13 PM (13 years, 11 months ago)

seems ok but if u want to do something for your own sake just put another piece of tyvek over the one that u punched a hole in and then tape it down or sumpin. that should sufficiently blockout contams from the punctured tyvek. also only time will tell about if it does contam or not. patience :smile:


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- Abraham Lincoln: Speech in the Illinois House of Representatives, Dec 18, 1840.


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OfflineMitchnast
Trial by Madness
Male User Gallery Arcade Champion: Mahjong

Registered: 10/28/99
Posts: 8,572
Loc: Okanagan
Last seen: 6 days, 20 seconds
Re: will it contam? [Re: Mitchnast]
    #1195816 - 01/06/03 08:15 PM (13 years, 11 months ago)

oh yes, and the lids are plastic with a small hole in the top in the center, i put the syringe in on an angle and injected throught the very edge of the tyvex.


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OfflineMitchnast
Trial by Madness
Male User Gallery Arcade Champion: Mahjong

Registered: 10/28/99
Posts: 8,572
Loc: Okanagan
Last seen: 6 days, 20 seconds
Re: will it contam? [Re: socratesmind]
    #1195857 - 01/06/03 08:32 PM (13 years, 11 months ago)

well, i put some tyvex over the holes and sealed it up, so basically im counting on the presumption that the few dozen spores that may or may not have entered the jars when they had the spores injected or when i applied the outer seals do not establish on the inner tyvex seal and produce more spores and the spores between the inner and outer seal do not find their way through the pin-hole in the inner seal.
i should know in 3-4 days what the biz really is


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Amazon Shop for: ½ Pint Jars, Rye Grain

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

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