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Offlinegangles
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Registered: 10/16/09
Posts: 7
Last seen: 14 years, 9 months
questions on jar incubation and monotubs.
    #11947397 - 02/02/10 11:45 AM (14 years, 11 months ago)

Ive been a member readin for a while, this is my first post though.

Im trying to get started doing the WBS tek, and eventually get to the 50/50 verm/peat casing in a monotub. I got syringes from one of the sponsers. I just have a few questions/comments.

I prepared 4 WBS jars hoping to use 3 for the monotub and 1 to start some more jars via G2G. I followed the doc WBS tek pretty closely except I didnt remove the black sunflower seeds. I have read opinions both for and against the BSS, I figured as long as I germinated the endospores and sterilized everything properly there shouldnt have been an issue. I inoculated in a glovebox about 12 days ago. Like the noob I am i did peak at the jars after 3 days and then every couple of days after that. I thought everything was going pretty good. but ill be sure not to peak next time..

But, I disposed of the jars today due to contamination. It wasnt trych it was an orangey goo type of contam. Not exactly positive what it is but I didnt really read up on it either.

I kept things as sterile as I could. Glove box, I put coffee filters on top of the jars after I inoculated, there was a layer of tyvek. Granted my lids were pretty funky, so that could have been one issue, I have since made new lids.

However I feel like my biggest issue was the temperature of incubation. And my following questions are mostly in regard to incubators. I have read opinions varying about colonization temp. It seems colonizing at room temp above 70 is fine.

I have been monitoring the temp in my grow area and it fluctuates between about 69 F - and 73, usually on the lower side. So im right at about 70 F most of the time, and have decided i should invest in an incubator.

I have read one method of filling a tub with water, heating with a fish tank heater, and then submerging another tub on top of it, tying down with zip tyes and placing jars etc in this tub.
    -do you think that this will create a significant loss of moisture from the jars? (i have read incubating does...)
    -is placing the jars directly in the water, with bleach or some other sterilizing agent in the water, a better method because the humidity in the tub will decrease the loss of moisture in the jars?

what about incubating monotubs?
    -incubating jars seems rather easy but is this tub submerging method feasible for monotubs? probably not...
  - is there a better method for controlling the temp in monotubs? i think its really most important during the initial colonization of the casing material. then the drop to room temp will just be an easy indicator.

im also starting to think about the humidity when i get to that point.

what do people think of having an inch layer of perlite, and the substrate/casing material on a casserole tray on top of the perlite, within a monotub?

just some thoughts, this way there will be an edge around the substrate/casing that the CO2 can drop around and exit through the vents when it comes time to fruiting. the perlite will increase the relative humidity, but this edge will also prevent condensation from dripping down the sides onto the mycelium.
    i just realized it but i guess this method is alot like people using the black gardening trays... which you can cut and tape to any size you want. probably better

I just dont want to get to the point of colonizing the casing and have to worry about the humidity, once its time to fruit i could just mist it but i want to prevent the FAE during colonization.


sorry if im a bit long winded. any help would be appreciated,
thanks!!


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Invisiblebiologys
Mycologist in Trainning
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Registered: 12/21/09
Posts: 4,622
Re: questions on jar incubation and monotubs. [Re: gangles]
    #11947427 - 02/02/10 11:51 AM (14 years, 11 months ago)

69-72 is fine i wouldn't worry about a incubator, as well in a monotub the substrate itself retains the moisture to keep the rh up..although sometime you may need to mist and fan a bit...

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Offlinegangles
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Registered: 10/16/09
Posts: 7
Last seen: 14 years, 9 months
Re: questions on jar incubation and monotubs. [Re: biologys]
    #11947439 - 02/02/10 11:53 AM (14 years, 11 months ago)

ok thanks!

is it still ok to mist monotubs during the casing colonization? i thought you should basically set it and forget it, and prevent the FAE.


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Invisibledrainhaven
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Registered: 03/31/09
Posts: 297
Loc: SouthernMan
Re: questions on jar incubation and monotubs. [Re: gangles]
    #11947462 - 02/02/10 11:57 AM (14 years, 11 months ago)

Dont get crazy...your jars at room temp are fine. Its not that. I would suggest not using funky lids and making sure the WBS is not too wet. Use a paper towel if you have to. I dont simmer either-personal preferance.

Just do a generic ohmatic monotub, save mad scientist stuff for agar, or future projects. Monotubs are easy dont over think it.

"what do people think of having an inch layer of perlite, and the substrate/casing material on a casserole tray on top of the perlite, within a monotub? "

-Is that a riddle? jk man


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"We are not human beings having a spiritual experience; we are spiritual beings having a human experience." -Anonymous

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Invisiblebiologys
Mycologist in Trainning
Male

Folding@home Statistics
Registered: 12/21/09
Posts: 4,622
Re: questions on jar incubation and monotubs. [Re: gangles]
    #11947481 - 02/02/10 12:01 PM (14 years, 11 months ago)

Quote:

gangles said:
ok thanks!

is it still ok to mist monotubs during the casing colonization? i thought you should basically set it and forget it, and prevent the FAE.




i asked the same question as i have a mono that i spawned and is colonizing the answer i recieved was that yes you could mist a bit if you thought it was too dry but it was best to  leave alone until fully colonized then mist or dunk to get moisture back.

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Offlinegangles
neighborhood friend
Male
Registered: 10/16/09
Posts: 7
Last seen: 14 years, 9 months
Re: questions on jar incubation and monotubs. [Re: drainhaven]
    #11953233 - 02/03/10 09:35 AM (14 years, 11 months ago)

@drainhaven you dont simmer at all? just rinse soak rinse PC? what about the black bird seed do you take that out?


Quote:

"what do people think of having an inch layer of perlite, and the substrate/casing material on a casserole tray on top of the perlite, within a monotub? "

-Is that a riddle? jk man




hahah im always over thinking i guess.. :grin:


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Offlinestarcade
Paladin


Registered: 06/28/10
Posts: 495
Last seen: 6 years, 15 days
Re: questions on jar incubation and monotubs. [Re: gangles]
    #13029669 - 08/09/10 11:27 PM (14 years, 5 months ago)

I did the same thing..

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