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OfflineCurious_George
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Registered: 07/15/02
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syringe making idea ???
    #1194593 - 01/06/03 05:26 AM (14 years, 4 months ago)

Hello All.

Been thinking a bit ( yeah i do this sometimes... ... ) :smile:

Instead of making a print, o'l fashion way,, would it be possible to cut a fresh, clean cap, dry it upside down ( gills up ) in a clean container, and when ready, take the cap and throw it into a sterile jar with water, shake it up and suck the purple water up with an empty syringe?

I've tried to make tea before and noticed that when you use a cap, you end up with purple water, LOTS of spores... this would make perfect syringes!!

I tried last night with a fresh cap, and it's no use, the spores are stuck to the gills and won't drop.

any good use to this?? seems like a good idea for those who don't have a flow hood and want to make a syringe with as little air exposure as possible..

thoughts!!

cg


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InvisibleBilge
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Registered: 08/26/02
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Re: syringe making idea ??? [Re: Curious_George]
    #1194623 - 01/06/03 05:46 AM (14 years, 4 months ago)

i'd think by the time the cap is dry it is also hopelessly contaminated.


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OfflineCurious_George
You want abaloon?

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Re: syringe making idea ??? [Re: Bilge]
    #1194624 - 01/06/03 05:49 AM (14 years, 4 months ago)

Why?

It would be grown in sterile conditions! cut with a clean knife and dried in a clean ( lysol,alcohol. whatever ) washed container with a lid.

pls explain!

cg


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InvisibleDreaMaTrix
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Re: syringe making idea ??? [Re: Curious_George]
    #1194824 - 01/06/03 02:16 PM (14 years, 4 months ago)

Just take a spore print in the bottom of a jar instead of an upside down dried out cap  :smirk:



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OfflineBlueJay
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Re: syringe making idea ??? [Re: DreaMaTrix]
    #1195011 - 01/06/03 03:09 PM (14 years, 4 months ago)

"Just take a spore print in the bottom of a jar instead of an upside down dried out cap  " :grin: :grin: :grin: :grin: :grin: :grin: :grin: :grin:

Gerber in fact with a drilled hole only slightly larger then the needle and near the side so you can tip it on its side to get that last syringe full again...You usually can get 60 cc into one jar(6 syringes per print..Perfect.. I use masking tape over my hole and peel back,wipe needle with alcohol and inject cooled strerile water in pulling back the spore filled water.....THE MOST IMPORTANT THING IS YOU MUST MAKE IT RIGHT AFTER IT DROPS!!! if you dont the spores will stick to the glass like they were super glued..The only draw back...


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Edited by BlueJay (01/06/03 03:11 PM)


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OfflineRaadt
nicht

Registered: 06/07/02
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Re: syringe making idea ??? [Re: DreaMaTrix]
    #1195019 - 01/06/03 03:11 PM (14 years, 4 months ago)

When you do this, make sure that you let the print dry. If it is wet, the spores seem to be electrostatically charged to the glass, and will not BUDGE when water is put into the jar. take the print, let it dry, scrape it about, then add water.


--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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OfflineCurious_George
You want abaloon?

Registered: 07/15/02
Posts: 1,065
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Re: syringe making idea ??? [Re: Raadt]
    #1196508 - 01/07/03 03:15 AM (14 years, 4 months ago)

Cool!!!

right-on! that will work.  THANKS!!

I really did not want to do the scrape spores into water in open air thing, this is awsome.

My spores droped on sunday night, i'll be using them tonight!  About the stuck in the bottom of jar thing.. i thought of this .. any possible harm with leaving the cap in the jar and adding water, shake it up and use the cap to rub against the glass to release the print in the water? or should i remove the cap first and use something else to dislodge the print?

cg ( yeah i know,, first timers are always the dumbest ones!!  :smirk: )


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InvisibleDreaMaTrix
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Re: syringe making idea ??? [Re: Curious_George]
    #1196939 - 01/07/03 07:48 AM (14 years, 4 months ago)

So, we have a jar with spores stuck to the bottom.
Put a few coins or pieces of glass in the bottom of the jar, sterilise it, then take your spore print. Remove cap, add sterile water, then swirl the coins/glass to set them spores free :smile:

Good luck



--------------------





"We are the one's we have been waiting for" - Hopi saying


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OfflineCurious_George
You want abaloon?

Registered: 07/15/02
Posts: 1,065
Loc: La La Land
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Re: syringe making idea ??? [Re: DreaMaTrix]
    #1197041 - 01/07/03 08:45 AM (14 years, 4 months ago)

:laugh:    :smile:    :cool:    :wink:

oky then!!

thanks again..

you know... most questions one knows the answer, but it just feels better to know that it's ok to do!!! haha..
cg


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Invisiblemycofile
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Re: syringe making idea ??? [Re: Curious_George]
    #1197281 - 01/07/03 10:32 AM (14 years, 4 months ago)

Actually, you can do pretty much exactly what you initially asked. In fact stamets talks about a similar method in GGAMM called spore mass innoculations. Basically you fruit in a jar so the fruit is sterile. Then take a mature cap, throw it in some water and shake it. Walla, heavily spored water.

Actually, he adds molasses to the water to make a liquid innoculum which is then multiplied about a 1000 times and used to innoculate lotsa grain or sawdust.

Whatever, your initial question is feasible.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

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OfflineCurious_George
You want abaloon?

Registered: 07/15/02
Posts: 1,065
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Re: syringe making idea ??? [Re: mycofile]
    #1197314 - 01/07/03 10:44 AM (14 years, 4 months ago)

small note.

IF the cap is FRESH,, it does not drop spores,, i tried it!!

However, if the cap dries out a bit, then YES SIR..  :laugh:

cg


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OfflineRaadt
nicht

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Re: syringe making idea ??? [Re: Curious_George]
    #1197331 - 01/07/03 10:49 AM (14 years, 4 months ago)

let the cap mature to the point of mass sporulation, and it drops when fresh  :wink:


--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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OfflineCurious_George
You want abaloon?

Registered: 07/15/02
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Re: syringe making idea ??? [Re: Raadt]
    #1197362 - 01/07/03 10:59 AM (14 years, 4 months ago)

i wanted to, but did not want spores all over my casing!!!

the veil was completely broken off and was an inch below the cap, when EXACTLY do the spores drop by themselves???

I was going to make a foil umbrella to attach to the stem just in case, but i did not.... felt kind of stupid!! maybe not!!

cg


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OfflineRaadt
nicht

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Re: syringe making idea ??? [Re: Curious_George]
    #1197742 - 01/07/03 01:18 PM (14 years, 4 months ago)

once the cap starts turning purple, you'll see spores start to accumulate on the casings... I honestly do not think it effects anything, but that's only me.


--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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OfflineOn_the_Down-Low
Polyfil-ophile-Say it 3 timesfast.

Registered: 10/15/02
Posts: 401
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Re: syringe making idea ??? [Re: Curious_George]
    #1199651 - 01/08/03 06:51 AM (14 years, 4 months ago)

I thought I read here that spores dropped on a casing will prevent fruit developement. But again, this is 2nd hand info.


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OfflineRaadt
nicht

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Re: syringe making idea ??? [Re: On_the_Down-Low]
    #1199660 - 01/08/03 06:55 AM (14 years, 4 months ago)

I've read it too, but have yet to see any evidence. It is very well possible, but from my own experiences i've not noticed it to be a problem.


--------------------
Raadt

-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--


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OfflineCurious_George
You want abaloon?

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Re: syringe making idea ??? [Re: Raadt]
    #1199777 - 01/08/03 07:55 AM (14 years, 4 months ago)

Hmmm...

Well, i made my first syringes last night.. worked ok,, a bit akward tho, since it was my first time  :blush: *( caugh: virgin ).

But it seems to have worked. i cleaned a penny with lysol, then with alcohol, then with distiled water, poped it in the plastic jar with the print on it, and shook it around, filled it with the water from my syringes that were filled and emptied with alcohol a few times, then filled and emptied with boiling hot water out of the microwave, i let them cool, and emptied and re-filled with water and spores...

I injected some jars right away with one of them, i had not read that you should wait 12 hours before using,, but should still work.

MUCH darker than the ones i baught!!!! they are purple!!! cool!

thanks for all the help guys!!

cg


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OfflineCurious_George
You want abaloon?

Registered: 07/15/02
Posts: 1,065
Loc: La La Land
Last seen: 12 years, 2 months
Re: syringe making idea ??? [Re: Curious_George]
    #1199782 - 01/08/03 08:00 AM (14 years, 4 months ago)

oh, about spores on casing, i think it's just ugly to have purple caps from the mushied below the open caps, but other than that, i can't see it being a problem!!

Worst case, they would germinate and create more mycelium!!! or not???  :confused:

we shal find out tho!!

cg

ps :: MAAAAAN these suckers grow fast!! they gre over 2 inches in 24 hours!!!! jesus christ! i love straw.. never again will i use BRF cakes.. NEVER
!!!!


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Here Johny,, have a joint.. all your friends are doing it!!


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InvisibleIDontGrow
insomniac

Registered: 09/23/02
Posts: 217
Re: syringe making idea ??? [Re: Curious_George]
    #1201263 - 01/08/03 03:44 PM (14 years, 4 months ago)

its called the 9er print tek....look it up


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Invisiblemycofile
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Re: syringe making idea ??? [Re: Curious_George]
    #1214382 - 01/13/03 12:37 PM (14 years, 4 months ago)

Heavily (and I mean much more heavily than one or two prints worth) sporulated casing surfaces do prevent or reduce further fruiting.

The real reason I would be concerned is that I've read professional references to spore covered casing encouraging the spread of viri. BTW, if you think contams are bad, you've never fought a virus. Spores are contaminated as well, not just cultures, and flow hoods are useless against them. They can wipe out entire strain libraries. Scarry stuff. But you shouldn't have to really worry about it, I did once, but can't even really blame it on a sporulated casing.


--------------------
"From a certain point of view"
-Jedi Master Obi Wan Kenobi

PM me with any cultivation questions.

I just looked at my profile and realized I had a website at one point in time on geocities, it's not there anymore and I have no idea what I had on it. Anybody remember my website from several years aga? PM if so please.


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