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Offlinehaze999
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Registered: 12/25/09
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sudden failure w/ inoculation
    #11937778 - 01/31/10 10:02 PM (11 years, 3 months ago)

Hi all, still on my first grow here.

I've knocked up 6 quart jars of WBS (3 MS, 3 LC) all the jars were successful.

Recently I wanted to try 7 more jars at once, so I used the same methods to knock them up with the LC I've used before, but none of the jars show any growth after about 4 days.

The only difference from the last 3 jars that were made from the same LC is that I didn't have time to simmer the grain (but I didn't simmer with the first 3 MS jars either...) and also the sponge I used to protect the needle inside my glove box was already used (although it was still soaked in 91% isopropyl.)

Could this small error with the sponge cause all 7 of the jars to fail?


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Invisiblefeelfunny
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Re: sudden failure w/ inoculation [Re: haze999]
    #11937804 - 01/31/10 10:07 PM (11 years, 3 months ago)

the sponge is not the problem. and just cuz it has been 4 days it dosent mean it wont work eather. what would make you think the sponge has anything to do with it??


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Offlinehaze999
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Re: sudden failure w/ inoculation [Re: feelfunny]
    #11937908 - 01/31/10 10:22 PM (11 years, 3 months ago)

last time I inoculated with this batch of LC I saw small growth after 24 hours and by 5 days at least 20% of the jar was colonized. But now 5 days later there is not a single tiny spec of growth in ANY of the new jars. :S. Those 3 jars from this LC are already 100% done 10 days later, so seeing as how there is no growth after 4 days at all something is wrong with all of the jars.


Edited by haze999 (01/31/10 10:26 PM)


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Re: sudden failure w/ inoculation [Re: haze999]
    #11937980 - 01/31/10 10:33 PM (11 years, 3 months ago)

are you shore the jars that are done arent mold? that sounds kind of fast even for a lc. any pics. did you suck myc into the syringe, i mean did you use all the myc in the LC for the first time?


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

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Offlinehaze999
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Re: sudden failure w/ inoculation [Re: feelfunny]
    #11938241 - 01/31/10 11:03 PM (11 years, 3 months ago)

100% it isnt mold, fluffy white. It's a strain that is known to be a fast colinizer. I used 10CC of LC the first time, and have about 300cc left. I boiled a syringe full of water then let it cool, when it was time for innoc I took the water out and filled it from the LC inside of my glove box. Also am 100% sure that there was myc floating inside the syringe the second time. I flame sterilized the needle in between each jar, and all my jar lids are self sealing with RTV silicone. I cooled the needle inside a alcohol soaked paper towel, then straight into the glove box, through the sponge into the jar.


Edited by haze999 (01/31/10 11:07 PM)


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