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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Pressure Cooker, Rye Grain, Vermiculite

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OfflinePinkFloyd
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Substrate jars slow
    #1192305 - 01/05/03 12:02 PM (13 years, 11 months ago)

The first time I grew, I used a kit (stupid) and all the jars grew out in 3-4 weeks. The kit contained 12 jars presterilized and I lost 3 to contams. When I realized how relatively easy it was I bought a pressure cooker and tried Hawk's formula on pint jars...none turned out. I was told here that pint jars were too big. Then I used 1/2 pint jars and after 2 months 2 jars grew through but were lost to contams shortly after I put them in the pod. I went back to square one and followed the PF Tek word for word 5 weeks ago and got 7 jars out of 19 grown through. The others are growing, but seem to have stagnated. None have gotten contams yet. What am I doing wrong? Steam sterilized for 45 minutes, the jars are taped...maybe not enough oxygen? Maybe this is normal and I just got lucky with the first strain. The first strain was B+ and now I am using Un Id from Joshua and Matias Romero from Captain Max. Maybe these strains are slower than B+. (The Un Id are the best colonizers yet). Just bought both of Stamlets books and am reading them now, but not much on cake cultures that I have found yet. I am thinking that I will try my hand at agar and grain spawn but am a little intimidated as cake culture is supposed to be the easyest and I'm having a little trouble.


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OfflineMickyFinn
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Registered: 12/19/01
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Re: Substrate jars slow [Re: PinkFloyd]
    #1192317 - 01/05/03 12:07 PM (13 years, 11 months ago)

in between innoculation and colonization, what type of method are you using to innoculate your jars? it sounds as if your area may be contaimenated. give us some more details on the innoculation part. have you been making your own syringes?


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OfflinePinkFloyd
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Re: Substrate jars slow [Re: MickyFinn]
    #1192839 - 01/05/03 04:07 PM (13 years, 11 months ago)

Last batch was innoculated with my own syringes from prints. I don't think contams due to none found in any of the jars. And 5 of the jars grew out in 4 weeks time.


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OfflineLoop_Theorist
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Re: Substrate jars slow [Re: PinkFloyd]
    #1192877 - 01/05/03 04:19 PM (13 years, 11 months ago)

you have a Pressure cooker, but your still steam serilizing? why?

If you are really sterillizing your jars at 15 PSI for at leat 45 mins, then the problem is your innoculation technique man.



-Loop


--------------------
Pack-tight, midnight, ship it to tha coast.
Some take tha plane, you, you on tha boat,
right beside tha money which right beside tha coke
Passin by tha coastgaurd, right under their nose.

To the US-HEY where anyone can getny, MIA all the way to New Your city, you a twenty now....


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OfflinePinkFloyd
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Re: Substrate jars slow [Re: Loop_Theorist]
    #1192928 - 01/05/03 04:41 PM (13 years, 11 months ago)

Yeah, I said steam steralized...but in the pressure cooker. I think my water level is off...or not enough oxygen


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OfflineEllis Dee
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Re: Substrate jars slow [Re: PinkFloyd]
    #1192959 - 01/05/03 04:49 PM (13 years, 11 months ago)

One of my newbie errors was to pack the verm/brf down to allow for the verm seal to be the amount in the recipe. If you pack the substrate at all it will colonize extremely slowly. Also IMO there is no need to pressure cook for 45 minutes at 15 PSI, 15 minutes is plenty at 15 PSI. Good luck.


--------------------
"If the foundations be destroyed, what can the righteous do."-King Solomon

And there was war in heaven: Michael and his angels fought against the dragon; and the dragon fought and his angels,


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OfflineAngry Mycologist
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Re: Substrate jars slow [Re: PinkFloyd]
    #1193538 - 01/05/03 07:31 PM (13 years, 11 months ago)

It sounds to me like there may be a problem with your substrate recipe. How much of each ingredient do you use? Also, where are you storing your jars?


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The proper penalty of ignorance, which is of course that those who don't know should learn from those who do... - Plato


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OfflinePinkFloyd
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Re: Substrate jars slow [Re: Angry Mycologist]
    #1194234 - 01/06/03 12:32 AM (13 years, 11 months ago)

PF Tek to the letter. Jars kept in an inc (rubbermaid) at exactly 86 degrees.


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OfflineAngry Mycologist
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Re: Substrate jars slow [Re: PinkFloyd]
    #1194238 - 01/06/03 12:40 AM (13 years, 11 months ago)

I'm not exactly sure what may be causing this problem. Maybe try lowering the temperature a bit? As the mycelium grows, it produces it's own heat, making the jars a bit warmer inside (not by much). I keep my incubator at 82...


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The proper penalty of ignorance, which is of course that those who don't know should learn from those who do... - Plato


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Offlineruskifile
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Re: Substrate jars slow [Re: Angry Mycologist]
    #1194271 - 01/06/03 01:10 AM (13 years, 11 months ago)

Yeah, I wondered about this too...when I used the stated substrate receipe it never really worked - 1/4 cup h20 was far too much for the verm i was using and I had to do much tweaking of the rye flour/water ratios until I got it nailed...
(brf was a total disaster from day one - some brands of rice flour are gritty not powdery to the touch and these really sucked :tongue:
I found rye flour was much better, with possibly more nutrients...)

I also think there's a big variation in types of vermiculite; one brand I had needed a totally different amount of h20 to another despite both being the 'same' grade

Just keep trying different ratios out if you're certain it's not the innoculating technique or your syringes 

...Oh yeah, maybe 86 is a little high considering it will be a couple of degrees higher inside the jars anyway. As AngryMycologist says lower 80's would probably be better  :grin:


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(zhukov in a previous life....)

2SER FM underground radio


Edited by ruskifile (01/06/03 01:14 AM)


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OfflinePinkFloyd
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Re: Substrate jars slow [Re: ruskifile]
    #1215076 - 01/13/03 05:00 PM (13 years, 10 months ago)

Figured out/fixed the prob. I loosened the caps of all the jars and let sit in the inc for another week. Either they were too moist and the excess water evaporated...or most likely they just needed a little more O2. Anyway they rocketed through the jars and all jars completed w/ no contams. Thanks for all the help/advise.


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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, Pressure Cooker, Rye Grain, Vermiculite

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