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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Vermiculite

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OfflineBladeLSD
Squirrel Master

Registered: 08/25/00
Posts: 801
Last seen: 6 months, 18 hours
My Jumbled Fumbled experiment
    #1191838 - 01/05/03 08:21 AM (14 years, 7 months ago)

on 12/12/02 I mixed 10, .5pint jars using this formula

1/8 cup of brown rice powder (
1/2 cup of horticultural vermiculite
40-45 cc's

Sterilized by boiling on low heat for 1 hour
!12/13/02 Innoculated each jar with 1cc PF Classic

Today I have 2 contaminated cake 4 cakes with small germination, 3 cakes with 20% but completly halted for atleast 2 weeks 1 cake with 50% but halted and 2 cakes with no growth at all

Anyone know whats going? Should I just ditch this experiment, and use my last syringe?(Strophoria straing which has been discontinued by PF)- should I use a different substrate formula this time?


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We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity :crazy:


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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: My Jumbled Fumbled experiment [Re: BladeLSD]
    #1191849 - 01/05/03 08:25 AM (14 years, 7 months ago)

Bigger jars take longer to colonize. The longer something takes to colonize the higher risk that foreign contaminants can invade. I will try 1/4 pint jars as soon as my spore syringe gets here and I will tell you all how it goes.


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->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
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OfflineBladeLSD
Squirrel Master

Registered: 08/25/00
Posts: 801
Last seen: 6 months, 18 hours
Re: My Jumbled Fumbled experiment [Re: SixCee]
    #1192003 - 01/05/03 09:42 AM (14 years, 7 months ago)

1/4 pint jars never seen them. Ill stick with the Half pint thanx


--------------------
We might get glimpse's of objectivity every now, and then, but we're so inherently locked in our temporal and corporeal selves that we're irrevocably locked into subjectivity :crazy:


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OfflineBC-Shroom
Evil Genius inTraining

Registered: 10/26/02
Posts: 127
Loc: BC, Canada
Last seen: 14 years, 4 months
Re: My Jumbled Fumbled experiment [Re: BladeLSD]
    #1192020 - 01/05/03 09:50 AM (14 years, 7 months ago)

Quote:

!12/13/02 Innoculated each jar with 1cc PF Classic

Today I have 2 contaminated cake 4 cakes with small germination, 3 cakes with 20% but completly halted for atleast 2 weeks 1 cake with 50% but halted 




After few weeks muselium probably used up all oxigen in the jars (if they tightly closed), that is why it stopped growing. Happens to me all the time, but its not a problem, I just losen lid a bit, and make sure that incubator is very clean. 
OR
You might have bacteriological (not mold-can't see it, only smell  :crazy: ) contamination. If that happened start all over again and improve your sterility technics.
Peace
BC 


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I regret to say that we of the F.B.I. are powerless to act in cases of oral-genital intimacy, unless it has in some way obstructed interstate commerce. - J. Edgar Hoover


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OfflineAngry Mycologist
Spontaneouslycombusting

Registered: 11/24/02
Posts: 1,282
Loc: Galapagos
Last seen: 14 years, 3 months
Re: My Jumbled Fumbled experiment [Re: BladeLSD]
    #1192225 - 01/05/03 11:29 AM (14 years, 7 months ago)

It wasn't too clear in your post as to whether or not you were using pint jars, or 1/2 pint jars. I'm assuming that since you said .5, you meant half-pint, if this is so, I would change my substrate recipe. Doesn't sound like you have enough BRF....or water for that matter. PF's maximum fruiting formula (for me at least): 1/4 BRF, 1/2 Vermiculite, 1/4 Water.


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The proper penalty of ignorance, which is of course that those who don't know should learn from those who do... - Plato


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Amazon Shop: ½ Pint Jars, Brown Rice Flour, Vermiculite

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