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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Two Weeks and Nothing!
    #1189882 -

I innoculated 12 1/2pint jars two weeks ago, 6 with B+ and 6 with Golden Teacher. I placed them in an incubator at about 80 degrees. Nothing is growing in the jars. I followed the PF Tek and used whole brown rice that I blended up alittle in a blender. The vermiculite in the jars seems a bit dry. Whenever I look at them the vermiculite seems to move around in the jars like it's completely dry. Should I start over and use more water?

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Re: Two Weeks and Nothing! [Re: MushroomGirl]
    #1189920 -

too dry... maybe wait it out a little to be sure then start over. i bet more help will be here in a bit.

What amount of water did u use.


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Edited by SubGen1us (01/04/03 09:27 AM)

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Re: Two Weeks and Nothing! [Re: SubGen1us]
    #1189939 -

if the mixture shakes around in the jars, then it is probably too dry


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TRIPPING IS FUN!

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Re: Two Weeks and Nothing! [Re: MushroomGirl]
    #1189945 -

Two weeks is a long time to wait for germination. It's difficult to say exactly where you went wrong unless you tell exactly how you prepped your jars.


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.

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Re: Two Weeks and Nothing! [Re: BigDaddy420]
    #1189947 -

I used 1/4 cup water for each 1/2pint jar. I think I read somewhere on here that you should use more water for fine vermiculite. Does anyone have any suggestions as to the water amount I should use when I do this again? And is it alright to use whole brown rice that has been run through a blender? Thanks.

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Re: Two Weeks and Nothing! [Re: MushroomGirl]
    #1189962 -

did u use some sort of dry heat for them? like a heat pad or light? if so that might be why the water dried out


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Re: Two Weeks and Nothing! [Re: SubGen1us]
    #1190011 -

Aaahhh real good question Sub!

Yeah explain to us your incubator setup.


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~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.

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Re: Two Weeks and Nothing! [Re: SubGen1us]
    #1190019 -

What you need to do is do away w/ all the measurements. If you are doing 6 jars then get 840 ml of verm. Then just dump water in until it is all wet, not pooled up, but all completely wet. Stir and make sure its even. Then add 240 ml of brf. Maybe the granule size of the brf is hindering your efforts. Try home depot, they have a 10 dollar grinder and maybe you will get a finer powder w/ that. Then it should mix a bit better. If not that, then buy a bag of it for 4 dollars. Its very fine. And make sure you dont compact it into jars, fluff it. If you do that you should be problem free for all of 2003.

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Re: Two Weeks and Nothing! [Re: RageOholic67]
    #1190027 -

Ummm you say do away with all measurements but then tell her to put in 840ml of vermiculite...

And ml is a measurement of liquids!

:crazy:


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~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.

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Re: Two Weeks and Nothing! [Re: BigDaddy420]
    #1190296 -

Quote:
if the mixture shakes around in the jars, then it is probably too dry


can't u just open and add a little water?

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Re: Two Weeks and Nothing! [Re: Robbyrob]
    #1190299 -

Quote:
can't u just open and add a little water?


sure you can, will you get contams? sure you may.

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Re: Two Weeks and Nothing! [Re: MickyFinn]
    #1190329 -


i also thought my jars may be a little dry ( pf tek ) but am afraid to open them due to contams ( good point Micky ) patience is a virtue, or so they say - wait a little longer and see what happens

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Re: Two Weeks and Nothing! [Re: DSD]
    #1190339 -

Inject in sterile water...


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~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.

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Re: Two Weeks and Nothing! [Re: MushroomGirl]
    #1190857 -

I had some jars that did not germinate right away. I made some more and they did well, but I kept the noshows in the incubator anyway (did not need the jars). After about A month some small growth was present. Thought it might have been a contam or somthing but it looked ok. Turned out to be some nice growth. Only one out of six jars contamed.

So, if you dont need the jars, make another batch and start form there.

Best of Luck. AWG

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Re: Two Weeks and Nothing! [Re: MushroomGirl]
    #1191506 -

It is not a dry problem, especially if you used syringes to inoculate the cakes. Spores can germinate with very little umidity in the substrate.
Two weeks are a lot of time if temperature was right. To high or to low temperature can retard germination of spores. You should double check your temperature especially on the bottom of the incubator.

or:

- The spores can be dead in the syringes, this can happen sometime. Or they are to old and need more time to germinate.

- You got them contamined from some invisible bacteria.

My suggestion is to carefully adjust temperature around 80 and wait some time. Also prepare some new jars using different and new syringes.

Byez


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MONTEZ

All what I've wrote can be true, false or unprecise. It is your choice to decide if believe me or not...

Edited by Montezuma (01/05/03 12:31 AM)

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Re: Two Weeks and Nothing! [Re: Montezuma]
    #1191687 -

Thanks all for the good info. I'm going to do 6 of them over and add a heating pad under my incubator. Right now all I have is a fish tank heater in a large glass vase full of water. The temps in the incubator stay around 75-80. What is an effective but fairly cheap way to incubate these jars properly? Again, thanks for the help, hopefully I'll have success on the next attempt.

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Re: Two Weeks and Nothing! [Re: MushroomGirl]
    #1191850 -

What is an effective but fairly cheap way to incubate these jars properly?

not every house/apt has this type of set up. but there are a lot that does

if you have the little storage space above the refridgerator, i found that to be a perfect spot for incubation (plenty of room also). the heat from the radiator thing on the back of the fridge keeps the temps just right. it fluxuates just a bit, but not enough to do anything bad.

hope that helps. cuz its a free incubation place if you got it

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Re: Two Weeks and Nothing! [Re: ribbit]
    #1191856 -

Wow great idea! :smile:


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~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.

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Re: Two Weeks and Nothing! [Re: SixCee]
    #1191865 -

if there is contams wont it contaminate the food?

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Re: Two Weeks and Nothing! [Re: Robbyrob]
    #1191866 -

Yes. Some contams aren't as agressive as others and won't attack the mycelium but will rather stop when they meet.


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.

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Re: Two Weeks and Nothing! [Re: Robbyrob]
    #1191903 -

Quote:
if there is contams wont it contaminate the food? 


No, don't worry about it. There is a lot of contams in the air anyway (unless you live in biological Level 3 Lab :smile: , or running air filters), there is a lot of contams in the fridge itself too...
Peace
BC 


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I regret to say that we of the F.B.I. are powerless to act in cases of oral-genital intimacy, unless it has in some way obstructed interstate commerce. - J. Edgar Hoover

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Re: Two Weeks and Nothing! [Re: ribbit]
    #1192177 -

Excellent idea ribit. I do have a cabinet above the refrigerator. I'll place a thermometer up there to see how warm it stays. Thanks. :laugh: 

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Re: Two Weeks and Nothing! [Re: MushroomGirl]
    #1192187 -

Have you tried Anno's incubator tek? It works wonders, and since you already have a heater, you'd only need another tub. Check it out: http://www.fungifun.org/pf/incubator.htm


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The proper penalty of ignorance, which is of course that those who don't know should learn from those who do... - Plato

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Re: Two Weeks and Nothing! [Re: MushroomGirl]
    #1194375 -

Alien's incubator works very well too... :grin:



aliens    


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(zhukov in a previous life....)

2SER FM underground radio

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Re: Two Weeks and Nothing! [Re: ruskifile]
    #1194678 -

I dumped out the old, uncolonized substrate today. It was very dry and there was nothing growing at all. I redid 4 of the jars and added more water this time. I will innoculate them when the pressure cooker is fully cooled. I think the problem was either wrong moisture content or the spores suck. I used partially ground up brown rice so I think the larger rice granules probably absorbed alot of the water causing the vermiculite to dry out. We'll see in about 10 days or so. Thanks for the info on the incubator, I'll try that out for sure. :wink: 

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Re: Two Weeks and Nothing! [Re: MushroomGirl]
    #1194719 -

definitely sounds like it's too dry.
b+ also takes a while to get started. we've had some batches that took about 4 weeks to get going.

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Re: Two Weeks and Nothing! [Re: girlskater]
    #1194819 -

Quote:
b+ also takes a while to get started. we've had some batches that took about 4 weeks to get going. 


This is not true but it's just what has happened to you. Usually spores germinate within 5-6 days up to two weeks maximum or something had gone wrong.

Aboute the rice, you should grind the grains as flour in order to have a good result and good moistness as well.

Byez :smile:


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MONTEZ

All what I've wrote can be true, false or unprecise. It is your choice to decide if believe me or not...

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Shop: Myyco.com Golden Teacher Liquid Culture For Sale   North Spore Bulk Substrate   Sporeworks.EU Spores for European Microscopy   Mushroom-Hut Liquid Cultures   Unfolding Nature Unfolding Nature: Being in the Implicate Order   MagicBag.co All-In-One Bags That Don't Suck

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