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OfflineMushroomGirl
Stranger
Registered: 12/08/02
Posts: 16
Last seen: 21 years, 4 months
Two Weeks and Nothing!
    #1189882 - 01/04/03 09:07 AM (21 years, 4 months ago)

I innoculated 12 1/2pint jars two weeks ago, 6 with B+ and 6 with Golden Teacher. I placed them in an incubator at about 80 degrees. Nothing is growing in the jars. I followed the PF Tek and used whole brown rice that I blended up alittle in a blender. The vermiculite in the jars seems a bit dry. Whenever I look at them the vermiculite seems to move around in the jars like it's completely dry. Should I start over and use more water?

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InvisibleSubGen1us

Registered: 11/26/02
Posts: 3,427
Re: Two Weeks and Nothing! [Re: MushroomGirl]
    #1189920 - 01/04/03 09:24 AM (21 years, 4 months ago)

too dry... maybe wait it out a little to be sure then start over. i bet more help will be here in a bit.

What amount of water did u use.


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Edited by SubGen1us (01/04/03 09:27 AM)

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OfflineBigDaddy420
nothing betterto do
Registered: 01/04/03
Posts: 17
Loc: here
Last seen: 21 years, 3 months
Re: Two Weeks and Nothing! [Re: SubGen1us]
    #1189939 - 01/04/03 09:33 AM (21 years, 4 months ago)

if the mixture shakes around in the jars, then it is probably too dry


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TRIPPING IS FUN!

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Invisibledog
straw dog

Registered: 06/20/02
Posts: 2,790
Loc: Route 66
Re: Two Weeks and Nothing! [Re: MushroomGirl]
    #1189945 - 01/04/03 09:35 AM (21 years, 4 months ago)

Two weeks is a long time to wait for germination. It's difficult to say exactly where you went wrong unless you tell exactly how you prepped your jars.


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Fascism (fash'izem) n. A governmental system marked by a centralized dictatorship, stringent socioeconomic controls, and often belligerent nationalism. see also: the Bush Administration.

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OfflineMushroomGirl
Stranger
Registered: 12/08/02
Posts: 16
Last seen: 21 years, 4 months
Re: Two Weeks and Nothing! [Re: BigDaddy420]
    #1189947 - 01/04/03 09:37 AM (21 years, 4 months ago)

I used 1/4 cup water for each 1/2pint jar. I think I read somewhere on here that you should use more water for fine vermiculite. Does anyone have any suggestions as to the water amount I should use when I do this again? And is it alright to use whole brown rice that has been run through a blender? Thanks.

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InvisibleSubGen1us

Registered: 11/26/02
Posts: 3,427
Re: Two Weeks and Nothing! [Re: MushroomGirl]
    #1189962 - 01/04/03 09:46 AM (21 years, 4 months ago)

did u use some sort of dry heat for them? like a heat pad or light? if so that might be why the water dried out


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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: Two Weeks and Nothing! [Re: SubGen1us]
    #1190011 - 01/04/03 10:12 AM (21 years, 4 months ago)

Aaahhh real good question Sub!

Yeah explain to us your incubator setup.


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->Quote of the Moment :
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OfflineRageOholic67
member
Registered: 11/07/02
Posts: 107
Last seen: 21 years, 3 months
Re: Two Weeks and Nothing! [Re: SubGen1us]
    #1190019 - 01/04/03 10:14 AM (21 years, 4 months ago)

What you need to do is do away w/ all the measurements. If you are doing 6 jars then get 840 ml of verm. Then just dump water in until it is all wet, not pooled up, but all completely wet. Stir and make sure its even. Then add 240 ml of brf. Maybe the granule size of the brf is hindering your efforts. Try home depot, they have a 10 dollar grinder and maybe you will get a finer powder w/ that. Then it should mix a bit better. If not that, then buy a bag of it for 4 dollars. Its very fine. And make sure you dont compact it into jars, fluff it. If you do that you should be problem free for all of 2003.

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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: Two Weeks and Nothing! [Re: RageOholic67]
    #1190027 - 01/04/03 10:18 AM (21 years, 4 months ago)

Ummm you say do away with all measurements but then tell her to put in 840ml of vermiculite...

And ml is a measurement of liquids!

:crazy:


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~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.

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OfflineRobbyrob
Cubensis seeker

Registered: 12/27/02
Posts: 653
Loc: Earth
Last seen: 14 years, 8 days
Re: Two Weeks and Nothing! [Re: BigDaddy420]
    #1190296 - 01/04/03 12:31 PM (21 years, 4 months ago)

Quote:

if the mixture shakes around in the jars, then it is probably too dry




can't u just open and add a little water?

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OfflineMickyFinn
Fuck the DJ
Registered: 12/18/01
Posts: 760
Loc: VA Beach VA
Last seen: 14 years, 2 months
Re: Two Weeks and Nothing! [Re: Robbyrob]
    #1190299 - 01/04/03 12:32 PM (21 years, 4 months ago)

Quote:

can't u just open and add a little water?



sure you can, will you get contams? sure you may.

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OfflineDSD
devil

Registered: 11/30/02
Posts: 1,263
Loc: Bad,Bad person - minus ...
Last seen: 5 years, 6 months
Re: Two Weeks and Nothing! [Re: MickyFinn]
    #1190329 - 01/04/03 12:46 PM (21 years, 4 months ago)


i also thought my jars may be a little dry ( pf tek ) but am afraid to open them due to contams ( good point Micky ) patience is a virtue, or so they say - wait a little longer and see what happens

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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: Two Weeks and Nothing! [Re: DSD]
    #1190339 - 01/04/03 12:55 PM (21 years, 4 months ago)

Inject in sterile water...


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.

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Anonymous

Re: Two Weeks and Nothing! [Re: MushroomGirl]
    #1190857 - 01/04/03 05:50 PM (21 years, 4 months ago)

I had some jars that did not germinate right away. I made some more and they did well, but I kept the noshows in the incubator anyway (did not need the jars). After about A month some small growth was present. Thought it might have been a contam or somthing but it looked ok. Turned out to be some nice growth. Only one out of six jars contamed.

So, if you dont need the jars, make another batch and start form there.

Best of Luck. AWG

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InvisibleMontezuma
Perennial pupil

Registered: 12/14/02
Posts: 26
Loc: Somewhere around the worl...
Re: Two Weeks and Nothing! [Re: MushroomGirl]
    #1191506 - 01/05/03 12:29 AM (21 years, 4 months ago)

It is not a dry problem, especially if you used syringes to inoculate the cakes. Spores can germinate with very little umidity in the substrate.
Two weeks are a lot of time if temperature was right. To high or to low temperature can retard germination of spores. You should double check your temperature especially on the bottom of the incubator.

or:

- The spores can be dead in the syringes, this can happen sometime. Or they are to old and need more time to germinate.

- You got them contamined from some invisible bacteria.

My suggestion is to carefully adjust temperature around 80 and wait some time. Also prepare some new jars using different and new syringes.

Byez


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MONTEZ

All what I've wrote can be true, false or unprecise. It is your choice to decide if believe me or not...

Edited by Montezuma (01/05/03 12:31 AM)

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OfflineMushroomGirl
Stranger
Registered: 12/08/02
Posts: 16
Last seen: 21 years, 4 months
Re: Two Weeks and Nothing! [Re: Montezuma]
    #1191687 - 01/05/03 04:57 AM (21 years, 4 months ago)

Thanks all for the good info. I'm going to do 6 of them over and add a heating pad under my incubator. Right now all I have is a fish tank heater in a large glass vase full of water. The temps in the incubator stay around 75-80. What is an effective but fairly cheap way to incubate these jars properly? Again, thanks for the help, hopefully I'll have success on the next attempt.

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Offlineribbit
up till dawn

Registered: 10/31/02
Posts: 290
Last seen: 12 years, 6 months
Re: Two Weeks and Nothing! [Re: MushroomGirl]
    #1191850 - 01/05/03 06:26 AM (21 years, 4 months ago)

What is an effective but fairly cheap way to incubate these jars properly?

not every house/apt has this type of set up. but there are a lot that does

if you have the little storage space above the refridgerator, i found that to be a perfect spot for incubation (plenty of room also). the heat from the radiator thing on the back of the fridge keeps the temps just right. it fluxuates just a bit, but not enough to do anything bad.

hope that helps. cuz its a free incubation place if you got it

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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: Two Weeks and Nothing! [Re: ribbit]
    #1191856 - 01/05/03 06:28 AM (21 years, 4 months ago)

Wow great idea! :smile:


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.

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OfflineRobbyrob
Cubensis seeker

Registered: 12/27/02
Posts: 653
Loc: Earth
Last seen: 14 years, 8 days
Re: Two Weeks and Nothing! [Re: SixCee]
    #1191865 - 01/05/03 06:32 AM (21 years, 4 months ago)

if there is contams wont it contaminate the food?

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InvisibleSixCee
keep rolling
Male

Registered: 06/12/02
Posts: 3,720
Loc: US, Chicago
Re: Two Weeks and Nothing! [Re: Robbyrob]
    #1191866 - 01/05/03 06:33 AM (21 years, 4 months ago)

Yes. Some contams aren't as agressive as others and won't attack the mycelium but will rather stop when they meet.


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.

Extras: Filter Print Post Top
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Shop: Myyco.com Golden Teacher Liquid Culture For Sale   Kraken Kratom Red Vein Kratom   Left Coast Kratom Buy Kratom Extract   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Liquid Cultures   North Spore Bulk Substrate   OlympusMyco.com Olympus Myco Bulk Substrate


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