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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Spores, LC, anastomosis?
#11877097 - 01/22/10 06:01 AM (14 years, 28 days ago) |
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I'm trying to wrap my head around the whole anastomosis thing. The idea is that compatible substrains within a multispore grow will exchange genetic material with each other and end up (more or less) identical. This is often used to explain when using fewer spores is a better option when inoculating, for example.
Ok, let's say I take an LC jar, and shoot in 1,000 spores. How many different genetic individuals might I reasonably expect to form? A million? (1000x1000) 62,500? (250x250) Hundreds? Several? Just one?
I understand it's a complicated scenario with a lot of variables- I'm asking for an educated guess, with explanation.
-------------------- You make it all possible. Doesn't it feel good?
Edited by Doc_T (01/22/10 06:08 AM)
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bardleyrichard
My Keyboard Got Damaged



Registered: 02/12/09
Posts: 1,904
Loc: Ohio
Last seen: 10 years, 2 months
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Re: Spores, LC, anastomosis? [Re: Doc_T]
#11877110 - 01/22/10 06:10 AM (14 years, 28 days ago) |
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I don't even know how you could figure this out, even if you somehow tested it
-------------------- I find myself yearning for clouds returning, all that the rain promises, and more...
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badman


Registered: 06/14/06
Posts: 4,039
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The possible variables are off the scale
100s
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Fahkface
Over-Fiend



Registered: 12/11/06
Posts: 4,821
Loc: In your Mind, Pedro! In y...
Last seen: 13 years, 4 months
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Re: Spores, LC, anastomosis? [Re: Doc_T]
#11877306 - 01/22/10 07:43 AM (14 years, 28 days ago) |
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My genetic understanding doesn't go too deep, but as far as I understand this (and in the end, I might be completely wrong about this), it's impossible to tell.
You shoot 1,000 genetic individuals into the LC and you're not able to recognize how many of them are compatible. All you'll see is, that mycelium will form.
The compatible monokaryotic mycelium strings combine by the process of anastomosis, share genetic information and form dikaryotic mycelium strings, making it a new strain of the species. Every monokaryotic cell contains multiple genetic information, that can result in different phenotypic characteristics (dominant / recessive genes).
I see no way how you should be able to tell how many compatible genetic individuals have formed or got lost.
But since preventing new genetic information from other mushrooms of the same species to enrich the genetic pool of a particular mushroom results in a degradation of the genetic line, it's to expect to be less than the 1,000 you shot in there (cause not all genetic individuals are compatible).
I might have neglected aspects, that don't appear to me (as I sometimes do ).
But with the information I could gather about this, this seems logical
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