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Offlineronjohn7779
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Magnetic Stirring Plate for LCs (What RPM should I use and how many hours should I use it?)
    #11874762 - 01/21/10 07:01 PM (14 years, 2 months ago)

I was lucky enough to be gifted a very nice lab quality magnetic stirring plate from a friend of mine.

I was wondering what RPM should I set it at?

And how many hours should my liquid culture be on the the stirring plate (i.e. 24/7 or 12/12)?


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Re: Magnetic Stirring Plate for LCs (What RPM should I use and how many hours should I use it?) [Re: ronjohn7779]
    #11874801 - 01/21/10 07:06 PM (14 years, 2 months ago)

I do 60 rpm once a day for 15 minutes. It's on a timer, I don't even touch the jars.


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Offlineronjohn7779
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Re: Magnetic Stirring Plate for LCs (What RPM should I use and how many hours should I use it?) [Re: Doc_T]
    #11874844 - 01/21/10 07:12 PM (14 years, 2 months ago)

Yikes this thing goes up to a thousand RPMs. I don't think it has such a low threshold. I think I might run this pitch 24/7 at 500 rpms.


--------------------
"Lennox Lewis, I'm coming for you man. My style is impetuous. My defense is impregnable, and I'm just ferocious. I want your heart. I want to eat his children. Praise be to Allah!" An American Hero Iron Mike!

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Re: Magnetic Stirring Plate for LCs (What RPM should I use and how many hours should I use it?) [Re: ronjohn7779]
    #11874986 - 01/21/10 07:30 PM (14 years, 2 months ago)

How is your fungus going to grow if you do that?
That would be like putting a grain jar in a paint shaker and just leaving it on.

You just have to stir it enough to break up the chunks and aerate the water a bit.


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Offlineronjohn7779
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Re: Magnetic Stirring Plate for LCs (What RPM should I use and how many hours should I use it?) [Re: Doc_T]
    #11875088 - 01/21/10 07:43 PM (14 years, 2 months ago)

Quote:

Doc_T said:
How is your fungus going to grow if you do that?
That would be like putting a grain jar in a paint shaker and just leaving it on.

You just have to stir it enough to break up the chunks and aerate the water a bit.



I'm actually fairly certain you want to agitate the shit out of a liquid culture. the more stirring the better. I was just looking for general consensus. From what I've read just now 300 rpms-24/7 is ideal.


--------------------
"Lennox Lewis, I'm coming for you man. My style is impetuous. My defense is impregnable, and I'm just ferocious. I want your heart. I want to eat his children. Praise be to Allah!" An American Hero Iron Mike!

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Re: Magnetic Stirring Plate for LCs (What RPM should I use and how many hours should I use it?) [Re: ronjohn7779]
    #11875138 - 01/21/10 07:50 PM (14 years, 2 months ago)

Ok, whatever works for you.


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