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InvisibleBlimeyGrimey
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I am has microscopy?
    #11787410 - 01/08/10 12:31 AM (14 years, 1 month ago)

I just received my Amscope B490 microscope. :awesome:

I started using it right away and finally got to look at some spores and bacteria cultures. The oil immersion works great. I'm using Cargille Type A at the moment.

Here's the first photo I took using my phone. I think one of my roommates has the real camera at the moment.

Spores from this Psathyrella species;




@ 1000x magnification:




Now I just need a reticle and stage micrometer. I saw a site selling "reticle disks". They looked like small lens filters with the reticle on them. Are these just laid into the eyepiece?

I'll be back with more pictures.


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Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.

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OfflineAlan RockefellerM
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Re: I am has microscopy? [Re: BlimeyGrimey]
    #11787509 - 01/08/10 01:04 AM (14 years, 1 month ago)

Quote:

Are these just laid into the eyepiece?




Yes.  Usually a little retainer ring is needed to hold the disc in place, but you could use a tiny bit of glue as well.

Be careful not to get immersion oil on the 40x objective.


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OfflinepsychedelicSLUG
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Re: I am has microscopy? [Re: Alan Rockefeller]
    #11787578 - 01/08/10 01:26 AM (14 years, 1 month ago)

SWEEET! :grin:


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:sun:Variety is the spice of life!:sun:



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InvisibleBlimeyGrimey
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Re: I am has microscopy? [Re: psychedelicSLUG]
    #11787802 - 01/08/10 03:38 AM (14 years, 1 month ago)

I realized the frame is a bit crooked on the base. Makes the stage move slightly diagonal to the base.

Also the damn label above the light intensity wheel came off when I pulled the blue protective sticker off of it. It only had one drop of glue to hold it on, I guess I'll have to super glue it back later.

I was considering returning the item but that'll end up costing me shipping which will make me have a loss in the end.

Why do I always get the defective/mis-assembled crap?


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Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.

Edited by BlimeyGrimey (01/08/10 04:10 AM)

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InvisibleBobzimmer
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Re: I am has microscopy? [Re: BlimeyGrimey]
    #11794651 - 01/09/10 07:32 AM (14 years, 1 month ago)

Congrats on the scope, Blimey!:handth:

Quote:

Why do I always get the defective/mis-assembled crap?



:lol: I hear ya...That's because everything is made in China nowadays. Even if the optics are made in Switzerland, chances are, the base was made in China or another similar second-world shithole.


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Mr. Mushrooms said:
I will confess something that should be quite obvious, CC.  I love mushrooms, i.e. fungi.  I really do.  I am talking about a strong feeling, i.e. emotion, for them.  I think they are beautiful.  I even dream of them.

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InvisibleWorld Spirit
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Re: I am has microscopy? [Re: BlimeyGrimey]
    #11794735 - 01/09/10 08:09 AM (14 years, 1 month ago)

:awesome:

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Offlinepsylosymonreturns
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Re: I am has microscopy? [Re: World Spirit]
    #11796261 - 01/09/10 01:40 PM (14 years, 1 month ago)

did you get it brand new or off craigslist or something?


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InvisibleBlimeyGrimey
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Re: I am has microscopy? [Re: psylosymonreturns]
    #11797481 - 01/09/10 05:11 PM (14 years, 1 month ago)

Quote:

Bobzimmer said:
Congrats on the scope, Blimey!:handth:

Quote:

Why do I always get the defective/mis-assembled crap?



:lol: I hear ya...That's because everything is made in China nowadays. Even if the optics are made in Switzerland, chances are, the base was made in China or another similar second-world shithole.




Quote:

psylosymonreturns said:
did you get it brand new or off craigslist or something?




Yeah, after doing a bit of research I've found out that Amscope's scopes are Chinese XSG models. My particular model is called the Amscope B490 but its real name is an XSG-109L, like the manual says. The slight mis-alignment isn't too bad and doesn't affect the optical properties at all. Not too bad of a scope for only $220 plus shipping.

Here's the scope itself;



and here's some of DannyGlick's Gymnopilus luteus spores @ 1000x;



--------------------
Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.

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OfflineChaos_ultt
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Re: I am has microscopy? [Re: BlimeyGrimey]
    #11797490 - 01/09/10 05:13 PM (14 years, 1 month ago)

Does that particular model come with the piece used to measure whatever it is you're viewing?

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InvisibleBlimeyGrimey
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Re: I am has microscopy? [Re: Chaos_ultt]
    #11797937 - 01/09/10 06:37 PM (14 years, 1 month ago)

No, it did not include a reticle or micrometer.

The site where I ordered my immersion oil sells reticles for $10 and the retaining ring is $6. I've seen a few WF10X eye pieces with reticles for about $25-30.

The biggest cost is the stage micrometer, cheapest one I've found is $65.


--------------------
Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.

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OfflineChaos_ultt
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Re: I am has microscopy? [Re: BlimeyGrimey]
    #11797946 - 01/09/10 06:42 PM (14 years, 1 month ago)

I think I'm eventually going to buy a scope from that site, but what you said re: the construction of your scope makes me a bit uneasy.

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InvisibleBobzimmer
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Re: I am has microscopy? [Re: Chaos_ultt]
    #11798041 - 01/09/10 07:03 PM (14 years, 1 month ago)

Quote:

Chaos_ultt said:
I think I'm eventually going to buy a scope from that site, but what you said re: the construction of your scope makes me a bit uneasy.



They're probably ALL made in China unless you're willing to shell out the really big bucks. Don't sweat it...like Blimey said, it doesn't affect the optics and if it comes down to it, the new ones have a warranty.


That's a pretty nice lookin' scope you got there Blimey! And your already taking some nice pics!:smile:


--------------------
Mr. Mushrooms said:
I will confess something that should be quite obvious, CC.  I love mushrooms, i.e. fungi.  I really do.  I am talking about a strong feeling, i.e. emotion, for them.  I think they are beautiful.  I even dream of them.

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OfflineAlan RockefellerM
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Re: I am has microscopy? [Re: BlimeyGrimey]
    #11798365 - 01/09/10 08:07 PM (14 years, 1 month ago)

Quote:

and here's some of DannyGlick's Gymnopilus luteus spores @ 1000x;




That picture looks great, I can almost see the roughness on the spores.

Quote:

The biggest cost is the stage micrometer, cheapest one I've found is $65.





Sometimes you can find them on ebay for $30, but I can send you mine.

Quote:

I think I'm eventually going to buy a scope from that site, but what you said re: the construction of your scope makes me a bit uneasy.




If you want a good scope, get an Olympus BH-2.

If you don't want to get one of those, an amscope will work just fine.

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InvisibleBlimeyGrimey
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Re: I am has microscopy? [Re: Alan Rockefeller]
    #11799355 - 01/09/10 11:23 PM (14 years, 1 month ago)

Quote:

Chaos_ultt said:
I think I'm eventually going to buy a scope from that site, but what you said re: the construction of your scope makes me a bit uneasy.




I tried turn the neck to be perfectly aligned with the base and it won't budge; which is a good thing. Its built very solid. The misalignment is actually only 2 degrees once I measured it. As you can see from the pictures, it works great.

The truth is, they are cheap Chinese scopes with good optics. In the end its the optics that matter and these are awesome for the price. There are toy microscopes that goes for similar prices with shitty optics and no mechanical stage.


Quote:

Alan Rockefeller said:
That picture looks great, I can almost see the roughness on the spores.




Thanks, I tried to focus it just right to show the roughness.


Quote:

Alan Rockefeller said:
Sometimes you can find them on ebay for $30, but I can send you mine.




Thanks, I'll let you know once I have a reticle to work with.



Ps. stuntzii @ 1000x:


Lepista nuda @ 1000x:


Pluteus sp. (possibly cervinus):



Anyone know a good stain for mycelium? Preferably to show the nucleus.


--------------------
Message me for free microscopy services on Psilocybe, Panaeolus, and Gymnopilus species.

Looking for wild Panaeolus cinctulus and Panaeolus olivaceus prints.

Edited by BlimeyGrimey (01/10/10 12:23 AM)

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