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 whiteshadow
Stranger
Registered: 12/02/09
Posts: 36
Last seen: 14 years, 13 days
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 using bamboo in master slants??? 
#11797796 - 01/09/10 06:07 PM (14 years, 3 months ago) |
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I cant find tongue depressors to cut up for my master slants I am making...but I do have a lot of bamboo skewers here. Will bamboo skewers work? I know pine is out of the question, and all the popsicle sticks I see are pine.
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Never order from university scientific, not even in a jam...they will rip you off.
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 lipa
Registered: 07/24/07
Posts: 2,684
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Re: using bamboo in master slants??? [Re: whiteshadow]
#11799579 - 01/10/10 12:32 AM (14 years, 3 months ago) |
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the pine will work fine!!.....! promise!!
Lipa
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mycochef
Stranger
Registered: 05/25/09
Posts: 223
Last seen: 12 years, 6 months
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Re: using bamboo in master slants??? [Re: lipa]
#11801179 - 01/10/10 10:56 AM (14 years, 3 months ago) |
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The popcicle sticks I get from my local craft store JoAnn's are hardwood(I'm almost 100% sure)and work great in my slants.
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artizen
JEFFERSONIAN
Registered: 12/16/08
Posts: 1,996
Loc: HOME SWEET HOME
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Re: using bamboo in master slants??? [Re: mycochef]
#11802094 - 01/10/10 01:33 PM (14 years, 3 months ago) |
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popcicle sticks for master slants. how does that work?
don't know if it applies i just bought these for obvious reasons.
http://www.mcfeelys.com/product/MG-1206/38quot-x-2quot-Multi-Groove-Dowel-Pin
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HCA AMU
IN LOVING MEMORY
1.6.1917 - 4.3.2010
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lipa
Registered: 07/24/07
Posts: 2,684
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Re: using bamboo in master slants??? [Re: artizen]
#11802546 - 01/10/10 02:47 PM (14 years, 3 months ago) |
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Search for roger rabbit and master slants. You should be able to find your answer.
Basically the mycelium permeates the stick and is able to survive for longer than the mycelium on the agar. It provides a protective barrier from outside elements and disturbance. Its like a fail safe for your culture in case the agar dries up.
Lipa
These are not hardwood sticks and the oyster run through them. I use these after inoculating them to slip behind the bark of freshly fallen willow trees in hopes to obtain fruits the next year.
Edited by lipa (01/10/10 02:59 PM)
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mycochef
Stranger
Registered: 05/25/09
Posts: 223
Last seen: 12 years, 6 months
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Re: using bamboo in master slants??? [Re: artizen]
#11802554 - 01/10/10 02:48 PM (14 years, 3 months ago) |
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I use 50X150mm test tubes and put a hydrated popcicle stick in it. Then I use a pipetter you could just as easily use a syringe to put 20cc in the tube with stick. PC the tubes in a TT rack when finished lay the rack with the test tubes down at roughly 15 degrees. You dont want the agar to go past the lip on the cap side. Let them cool and your done. I usually do 50 at a time so they'll always be ready.
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whiteshadow
Stranger
Registered: 12/02/09
Posts: 36
Last seen: 14 years, 13 days
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Re: using bamboo in master slants??? [Re: mycochef]
#11803045 - 01/10/10 04:03 PM (14 years, 3 months ago) |
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popsicle sticks it is!
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Never order from university scientific, not even in a jam...they will rip you off.
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nexus1946
Rebel
Registered: 06/25/09
Posts: 2,315
Loc: In the moment
Last seen: 7 years, 9 months
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Re: using bamboo in master slants??? [Re: lipa]
#11804327 - 01/10/10 07:28 PM (14 years, 3 months ago) |
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Quote:
lipa said:
Search for roger rabbit and master slants. You should be able to find your answer.
Basically the mycelium permeates the stick and is able to survive for longer than the mycelium on the agar. It provides a protective barrier from outside elements and disturbance. Its like a fail safe for your culture in case the agar dries up.
Lipa
These are not hardwood sticks and the oyster run through them. I use these after inoculating them to slip behind the bark of freshly fallen willow trees in hopes to obtain fruits the next year.
Willow eh? Cool!
Does it work well for ya'?
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Gypsum/Drywall Tek
The Story Of Russell The Texas (Cube) Bear
 
The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves.
-Arnold J. Toynbee
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noobieshroomie
Back again
Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure 
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Re: using bamboo in master slants??? [Re: nexus1946]
#11805232 - 01/10/10 10:15 PM (14 years, 3 months ago) |
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awesome info in here
im looking to make some masters soon here myself
@ mycochef 50 at a time huh
where would i find these 50x150mm tubes at?
and if you have any extra empty ones would you be up for a trade?
Pm me an let me know, thanks
Lipa thats a great idea as well
i like that bag it looks like a wooden porcupine
-noobie-
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AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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lipa
Registered: 07/24/07
Posts: 2,684
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Re: using bamboo in master slants??? [Re: nexus1946]
#11805595 - 01/10/10 11:42 PM (14 years, 3 months ago) |
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Quote:
nexus1946 said:
Quote:
lipa said:
Search for roger rabbit and master slants. You should be able to find your answer.
Basically the mycelium permeates the stick and is able to survive for longer than the mycelium on the agar. It provides a protective barrier from outside elements and disturbance. Its like a fail safe for your culture in case the agar dries up.
Lipa
These are not hardwood sticks and the oyster run through them. I use these after inoculating them to slip behind the bark of freshly fallen willow trees in hopes to obtain fruits the next year.
Willow eh? Cool!
Does it work well for ya'?
I'll tell you next year..
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nexus1946
Rebel
Registered: 06/25/09
Posts: 2,315
Loc: In the moment
Last seen: 7 years, 9 months
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Re: using bamboo in master slants??? [Re: lipa]
#11805676 - 01/10/10 11:57 PM (14 years, 3 months ago) |
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Quote:
lipa said:
Quote:
nexus1946 said:
Quote:
lipa said:
Search for roger rabbit and master slants. You should be able to find your answer.
Basically the mycelium permeates the stick and is able to survive for longer than the mycelium on the agar. It provides a protective barrier from outside elements and disturbance. Its like a fail safe for your culture in case the agar dries up.
Lipa
These are not hardwood sticks and the oyster run through them. I use these after inoculating them to slip behind the bark of freshly fallen willow trees in hopes to obtain fruits the next year.
Willow eh? Cool!
Does it work well for ya'?
I'll tell you next year..
Gotcha'.
Did you slip them behind the bark this fall?
Forgive the questions. I'm just not sure of the process and find it interesting. 
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Gypsum/Drywall Tek
The Story Of Russell The Texas (Cube) Bear
The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves.
-Arnold J. Toynbee
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 1 year, 1 month
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Re: using bamboo in master slants??? [Re: nexus1946]
#11806808 - 01/11/10 08:48 AM (14 years, 3 months ago) |
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Pine will work once it's been kiln dried. It's fresh pine logs which have anti-fungal properties that mycelium doesn't appreciate. I doubt the pine will last long in a slant, but time will tell. If it lasts a year, you should be making new ones by then anyway.
RR
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Download Let's Grow Mushrooms
semper in excretia sumus solim profundum variat
"I've never had a failed experiment. I've only discovered 10,000 methods which do not work."
Thomas Edison
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lipa
Registered: 07/24/07
Posts: 2,684
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Re: using bamboo in master slants??? [Re: nexus1946]
#11807123 - 01/11/10 10:27 AM (14 years, 3 months ago) |
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Quote:
Gotcha'.
Did you slip them behind the bark this fall?
Forgive the questions. I'm just not sure of the process and find it interesting.
Yes, and GPS'd the trees so I could come back to them next year. If it works well it will be a very easy way to make inoculation media that is easy to make and easy to apply other than using spores.
Process is simple. Just get a pot of boiling water going and throw the sticks in for about a half hour. Cool them. Poke a shitload of them into your fruiting block or bag. Wait 5 to 10 days and then remove and store in a plastic container with a moistened piece of cardboard or paper towel. Take during hikes to inoculate freshly fallen trees. I also look for those erosion control rice straw thingamabobers (the name escapes me) and poke all I have left after a hike into them. Always worth trying new things!!
Lipa
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mycochef
Stranger
Registered: 05/25/09
Posts: 223
Last seen: 12 years, 6 months
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Re: using bamboo in master slants??? [Re: lipa]
#11807648 - 01/11/10 12:16 PM (14 years, 3 months ago) |
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Test tubes are from Discountvials.com I will double check but I think my sticks are hardwood but Lipa stopped by yesterday and he feels they're pine. I'll find out, anyway they work great for me. Got something to trade I'll send you one. I have numerous Genera stored this way.
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crazyface
Adventurist
Registered: 06/11/10
Posts: 125
Loc: Cincinnati, OH USA
Last seen: 10 months, 12 hours
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Re: using bamboo in master slants??? [Re: RogerRabbit]
#22732367 - 01/03/16 03:05 PM (8 years, 3 months ago) |
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So what do you think about using bamboo? I have access tons of coffee stirrer sticks made from bamboo at my job. Will this suffice for a master slant?
Edited by crazyface (01/03/16 03:05 PM)
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