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InvisibleNecco
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Yes or no: to bother with slow growing non-rhizomorphic culture? (pics)
    #11791824 - 01/08/10 06:48 PM (14 years, 1 month ago)

A swab was swiped across some malt extract agar in a 1/2 pint. Only one colony showed up (leading me to believe that very few spores were knocked off the swab, or that hydration was necessary).

The colony that did show up is growing slow, at about half the speed of a different plate prepared at the same time (different strain as well). This colony is about 2.5 weeks old.

The colony that is present is very flat compared to others I've seen, and no branching rhizomorphic mycelia appear to be present. The strain is penis envy. The colony looks perfectly round; sorry for the poor pics, but it is really hard to get a good picture through a jar.

So would you ditch this colony and start over to get a colony that is more vigorous/fast/rhizomorphic/thick,
or do you think it is worth attempting to go all the way through harvest? :thumbup::thumbdown:

PE at 2.5 weeks:

For the sake of comparison, here is a plate of APE that was inoculated around the same time (a couple days earlier), but has been in the refrigerator since a week ago. It grew about 2X quicker and 3X thicker than the PE culture. It may show no rhizomorphic growth, but the colony is very thick and is not perfectly round. This was also from a swab-swipe that resulted in growth from one tiny area.

APE at 1.5 weeks:

Your input is appreciated.

edited for some degree of disambiguation


Edited by Necco (01/08/10 07:28 PM)


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Invisiblefeelfunny
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Re: Yes or no: to bother with slow growing non-rhizomorphic culture? (pics) [Re: Necco]
    #11791860 - 01/08/10 06:53 PM (14 years, 1 month ago)

it takes a few transfers to get this


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Offlineno_luck777
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Re: Yes or no: to bother with slow growing non-rhizomorphic culture? (pics) [Re: Necco]
    #11791867 - 01/08/10 06:54 PM (14 years, 1 month ago)

You know, I tried that before as well out of curiosity and I would definitely ditch it.

My experience was extremely slow mycelium growth with almost no fruit to speak of. Granted that was just one experience but there ya go. :smile:


Edited by no_luck777 (01/08/10 06:56 PM)


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Invisiblefeelfunny
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Re: Yes or no: to bother with slow growing non-rhizomorphic culture? (pics) [Re: no_luck777]
    #11791881 - 01/08/10 06:57 PM (14 years, 1 month ago)

yea a good one should fruit


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

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InvisibleNecco
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Re: Yes or no: to bother with slow growing non-rhizomorphic culture? (pics) [Re: no_luck777]
    #11791891 - 01/08/10 06:58 PM (14 years, 1 month ago)

Yeah, I'm more concerned about the extremely slow growth than I am about not seeing rhizos or the flatness. Overall it seems like a weak one, but I'm new to agar and don't know what to expect.


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Invisiblefeelfunny
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Re: Yes or no: to bother with slow growing non-rhizomorphic culture? (pics) [Re: Necco]
    #11791895 - 01/08/10 06:59 PM (14 years, 1 month ago)

most mine start like this


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

Feel Family Founder. :pm: me if you are tired of hearing, "Use the search function".


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OfflineRogerRabbitM
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Re: Yes or no: to bother with slow growing non-rhizomorphic culture? (pics) [Re: feelfunny]
    #11792035 - 01/08/10 07:23 PM (14 years, 1 month ago)

When you germinate spores on agar, there are anywhere from a few hundred to a few thousand strains piled on top of each other.  There's rhizomorphic strains there, but they're covered up by others.  Grab a few pieces of mycelium from around the leading edge of the growth and transfer to new plates.  Repeat a few times, and you'll start seeing your isolates.  I generally go through at least a few dozen dishes during the process.  You'll soon see the value of petri dishes over jars.
RR


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Invisiblefeelfunny
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Re: Yes or no: to bother with slow growing non-rhizomorphic culture? (pics) [Re: Necco]
    #11792044 - 01/08/10 07:24 PM (14 years, 1 month ago)

see thats what i was trying to show/tell you with not so many words


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IF A CAT AND DOG CAN GET ALONG WHY CANT EVERYONE ELSE?
If the sky is falling, don't look up!  :abduction:

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InvisibleNecco
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Re: Yes or no: to bother with slow growing non-rhizomorphic culture? (pics) [Re: RogerRabbit]
    #11792221 - 01/08/10 07:50 PM (14 years, 1 month ago)

Quote:

RogerRabbit said:
When you germinate spores on agar, there are anywhere from a few hundred to a few thousand strains piled on top of each other.  There's rhizomorphic strains there, but they're covered up by others.  Grab a few pieces of mycelium from around the leading edge of the growth and transfer to new plates.  Repeat a few times, and you'll start seeing your isolates.  I generally go through at least a few dozen dishes during the process.  You'll soon see the value of petri dishes over jars.
RR




Because it is assumed that there are so many strains present in a colony, I also assumed that colonies would average out and grow at similar rates (perhaps this is a poor assumption?).

My main concern was that out of 4 different plates started from spores on agar, 3 dishes showed thick colonies that grew about the same rate. This particular dish of PE, on the other hand, is taking a very long time to take over the plate and is thin. Another dish prepared in the same way from the same swab of PE showed no growth at all, giving me the impression that very few spore clumps were being released by the cotton. In other words, there may be a lot less diversity in this colony than we tend to expect, and the strains present may be poor performers (and not worth growing).

Do you suppose that there are vigorous strains being subdued by others?

I absolutely agree about the use of Petri dishes over jars. The moment I pulled these out of the pressure cooker I placed an order for some dishes because I realized how much jars suck to work with (& see through, and store, etc. etc.).

edit: disambiguation


Edited by Necco (01/08/10 08:55 PM)


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Offlineiluvfungi
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Re: Yes or no: to bother with slow growing non-rhizomorphic culture? (pics) [Re: Necco]
    #11792287 - 01/08/10 08:01 PM (14 years, 1 month ago)

Only way to be sure is to pour many plates and take the best regions.


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Re: Yes or no: to bother with slow growing non-rhizomorphic culture? (pics) [Re: iluvfungi]
    #11792350 - 01/08/10 08:13 PM (14 years, 1 month ago)

Why are you using cotton instead of an inoculating loop?
RR


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InvisibleNecco
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Re: Yes or no: to bother with slow growing non-rhizomorphic culture? (pics) [Re: RogerRabbit]
    #11792600 - 01/08/10 08:49 PM (14 years, 1 month ago)

Quote:

RogerRabbit said:
Why are you using cotton instead of an inoculating loop?
RR



:ashamed:
Because the spores are from a swab. I rubbed the swab right on the agar surface.

The search engine didn't give me any good descriptions of swab usage.


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InvisibleNecco
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Re: Yes or no: to bother with slow growing non-rhizomorphic culture? (pics) [Re: Necco]
    #11792837 - 01/08/10 09:29 PM (14 years, 1 month ago)

What would be the explanation behind the difference of these two different cultures if they are both composed of hundreds to thousands of dissimilar strains?:
slow growerfast grower

...same agar preparation, same incubation conditions, ~same inoculation time, method. No comprendo.

Sorry for the double post. This was an afterthought.


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InvisibleDanKnugget
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Re: Yes or no: to bother with slow growing non-rhizomorphic culture? (pics) [Re: Necco]
    #11794241 - 01/09/10 02:53 AM (14 years, 1 month ago)

I am also unclear on how to use a swab. I will look into it sometime tomorrow or sunday, and I will get back to ya if no one has posted how to use one in here.  Do you have to use a swab with agar, or can you somehow make it into a syringe?


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