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OfflinelosfreddyS
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Does it look like this cloning and agar are ok?
    #11788665 - 01/08/10 12:11 PM (11 years, 4 months ago)

So far so good?  what do i do next?



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Re: Does it look like this cloning and agar are ok? [Re: losfreddy]
    #11788717 - 01/08/10 12:23 PM (11 years, 4 months ago)

That tissue section- is there mycelium growing from it? Or is that bacteria?


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OfflinelosfreddyS
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Re: Does it look like this cloning and agar are ok? [Re: Doc_T]
    #11788751 - 01/08/10 12:31 PM (11 years, 4 months ago)

ya, the first pic is a tissue cutting.  may be hard to see, but there is mycelium growing.  IDK if its supposed to be more flush.  The 2nd pic is from spores.  I thought that that dark spot at the bottom was contam, but i think it may just be the spores.


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Re: Does it look like this cloning and agar are ok? [Re: losfreddy]
    #11788797 - 01/08/10 12:42 PM (11 years, 4 months ago)

Spore plate: find a good section, transfer to a new dish and isolate for further use.
Tissue plate: am I looking to close to the sample? Is the mycelium farther away, kinda thin?


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Re: Does it look like this cloning and agar are ok? [Re: Doc_T]
    #11788832 - 01/08/10 12:51 PM (11 years, 4 months ago)

ya.  its really light.  Its kinda hard to see even with the camera.  heres a pic from behind.  i was thinking a g2g from agar or agar to LC, but idk really.  Dont have too much experince with agar



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Re: Does it look like this cloning and agar are ok? [Re: losfreddy]
    #11788861 - 01/08/10 12:57 PM (11 years, 4 months ago)

this is where i would take samples from



      -noobie-


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AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef


Edited by noobieshroomie (01/08/10 01:04 PM)


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