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bobbin
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Registered: 12/29/09
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Does alcohol extraction work with mycelium?
#11777745 - 01/06/10 04:11 PM (14 years, 1 month ago) |
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Hello everyone.
I've read how to extract psilocybin from mushroms with alcohol. And how to do it from mycelium growing in rye cakes with cranberry juice. But does alcohol extract from mycelium growing in rice cakes? is safe safe? How's the potency compared with extracting from mushrooms?
Edited by bobbin (01/06/10 04:13 PM)
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veda_sticks
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Re: Does alcohol extraction work with mycelium? [Re: bobbin]
#11777793 - 01/06/10 04:18 PM (14 years, 1 month ago) |
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It depends on teh age of the mycelium. if its never fruited it wont have much actives at all. The last time i checked this out, it was generaly best to use mycelium that had atleast produced 1 flush.
Im pretty sure that a flush of mushrooms will contain alot more actives that the mycelium they grew from.
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bobbin
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Re: Does alcohol extraction work with mycelium? [Re: veda_sticks]
#11778036 - 01/06/10 04:51 PM (14 years, 1 month ago) |
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thanks for the answer.
So if the cake has never fruited it'll not have psilocybin? or just less?
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
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Re: Does alcohol extraction work with mycelium? [Re: veda_sticks]
#11778613 - 01/06/10 06:09 PM (14 years, 1 month ago) |
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It absolutely does and I've done it!
Here's a thread I made where I did the experiment, then discussed about the stability of psilocybin if I left it in the alcohol soultion (solvent)-> http://www.shroomery.org/forums/showflat.php/Number/10411553/page/3
One important thing to remember is that the mycelium has to be matured for awhile before its worth extracting.
Quote:
veda_sticks said: It depends on the age of the mycelium. If its never fruited it wont have much actives at all. The last time I checked this out, it was generaly best to use mycelium that had at least produced 1 flush.
I'm pretty sure that a flush of mushrooms will contain a lot more actives that the mycelium they grew from.
I would say that's debatable. Although I don't have any good data to argue that mycelium that has not been fruited yet has more active chemcials, logic tells me that during fruiting, organic chemicals such as Psilocybin (which is not a nutrient to the mushroom, but maybe a defensive mechanism?), travel from the mycelium to the mushroom as the mycelium supplies it with other real nutrients like water and protiens. Therefore, fresh non-fruited mycelium that has been aged (I do agree with veda_sticks on that one) would be most potent. Everytime I've done a mycelium extraction, its been from fresh, un-fruited mycelium.
Anyone here done any quantitative experiments on this subject? Wanna share? -----------------------------------------------------------------------------------------------------------------------------------------
As for Potency, its pretty powerful if you do it right, but you have to use a lot of mycelium...
Here's a rough estimate for dosage and intensity (from my personal experiments)
1 fully colonized half-pint cake (aged 1 week) = about a Level 1-2 out of 5 trip.
^ Its a pretty rough estimate, but that seems to be what I experience...
>> Anyway, back to the procedure you want to know about.....
Here's how you do a simple Alcohol Extraction (Simply Put)
1) Colonize a substrate 100% (I prefer grains) with P. cubenis mycelium from spores or a G2G transfer.
2) Wait about 1 week after full colonization for the mycelium to "strengthen" its network. This increases the psychoactive potency of the mycelium and makes more of it.
3) Buy 190 proof (95%) or above pure alcohol spirit (I use EverClear(r) for the job) and get a electric blender.
4) Cut up your colonized cake into the blender and pour a nice amount of the 190+ proof alcohol into the blender. Blend mixture to a smooth texture.
5) Now you have to make a double-boiler....Get 2 pots, one that fits inside the other, and one of those metal steamer baskets used for steaming vegi's (Its the ones that fold out like a flower). Put the metal steamer in the bigger pot, then fill the pot about half-way with water. Now, turn on the power on your stove-top to "HI", because you're about to get HI..(just jokin').
6) Now, pour the mycelium/alcohol/substrate blend from the blender into the smaller pot. Now, place other smaller pot inside the large one and let it heat up. Adjust the heat when the mycelium blend starts to form tiny bubbles, around "Med" to just above "Low" usually. The double-boiler is used because alcohol has a low boiling point, and you don't wanna burn or dry-out the mycelium. That would be bad.
7) Gentle stir for like an hour or so (the longer the better), adding alcohol to the mixture when the level gets low. Also, add water to underneath if that gets too low too. While its boiling, get a large bowl (or another pot) and a white t-shirt or *fine-thread* cloth you don't want anymore. This will be used for filtering out the grain/substrate from the now psychedelic alcohol.
9) Here's the tricky part...after your done boiling, while its hot (but not too hot or you'll get burned!), pour the mixture into the bowl/pot with the t-shirt stretched over it. The reason the filtering is done hot is because the alcohol flows better thru the cloth and prevents psilocybin crystals from forming, which form from cooling while your filtering the alcohol. Quickly twist the T-shirt into a ball and ring out the alcohol. Make sure its not right off the grill, or you'll burn your hands. Might be a good idead to wear thick rubber gloves at this stage for safety. Ring and squeeze the T-shirt dry into the pot/bowl. Repeat if there is liquid left in the cooking pot.
10) This is my favorite part...You can either drink it like that (I do not recomend it, since its hard alcohol and you won't be tripping: you'll just be drunk) or you can evaporate the alcohol (solvent). Evaporating is my recomendation.
Evaporating the Alcohol from the Extraction (Optional but Recomended)
1. Funnel the alcohol/actives solution into a wide glass, the more horizontal surface area, the better. Those wide coffee mugs or those fat-ass glasses work great, just make sure its pretty shallow.
2. Find a high-velocity fan and set it up over the glass. I use either a stack of books or tupperware to get the height and angle I want the fan to be. It should face the glass at about a 45 degree angle from over head.
3. Turn on the fan and watch for ripping on the surface of the alcohol in the glass. Move around the fan (pitch, elevation, etc.) until you get the most rippling effect possible.
4. The best part....sit back and watch as the alcohol level goes down. When I first did this, I started to see white crystals form at the bottom of the glass. It was pretty exciting because I knew it was the psilocybin/psilocyin molecules cooling down and forming together into visible "clumps". Also, you will notice white residue on the sides of the glass as it goes down. Thats actives too. Might want to scrape it off at the end of the fan-procedure.
5. Leave fan on until you get to a desired level. I couldn't get mind to just dry crystals, since its part water in the alcohol too. If you don't want *any* alcohol in the final product, give it a sniff test. If it smells like hard alcohol, keep the fan on. If it kinda smells like funky water, you are done evaporating.
And that's it! Simple right? I know it sounds pretty complicated from my step-by-step description, but its not too bad as long as you have several hours of free-time to kill.
Well, good luck and may the mycelium be your guide...
The Extraction Faction ~ LogicaL Chaos ~
Edited by LogicaL Chaos (01/06/10 06:26 PM)
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oh_you_know
lsd <3



Registered: 02/08/09
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Re: Does alcohol extraction work with mycelium? [Re: LogicaL Chaos]
#11897342 - 01/25/10 10:26 AM (14 years, 24 days ago) |
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well if im kinda thinking about it like a dmt extraction, when u have the trippy alco, instead of fan evaporating, why not stick in a super cold, cold as could be, freezer? in a dmt extraction the dmt crystals freeze and stick to the side, use half pint jars or something, freeze it, pour the alcohol into another jar and freeze again, then jsut keep scraping the crystals out of the jars. sounds like it would work in my head, maybe try it out? let us know how it goes.
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LogicaL Chaos
Ascension Energy & Alien UFOs




Registered: 05/12/07
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Re: Does alcohol extraction work with mycelium? [Re: oh_you_know]
#11897372 - 01/25/10 10:31 AM (14 years, 24 days ago) |
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Sounds plausible, and the science is there....
but how do you seperate the crystals from the alcohol? (if the precipitate from the mycelium/alcohol extraction is actually crystals)
And is it dry of the alcohol? Does the freezing "push-out" the solvent from the solution and make the "crystals" dry?
I'll definitely try it next time (soon) I do a mycelium extraction to see if its better than the fan evaporation method.
Oh, I do know...now ~ LogicaL Chaos ~
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oh_you_know
lsd <3



Registered: 02/08/09
Posts: 2,282
Loc: Oh, USA
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Re: Does alcohol extraction work with mycelium? [Re: LogicaL Chaos]
#11897420 - 01/25/10 10:40 AM (14 years, 24 days ago) |
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haha well im no scientist, i jsut know the dmt extract and applied it here just cause u cause them crystals lol well in dmt the paint thinner stays liquid and they are "dry" you pour it out and then let it air dry a bit and scrap them out. i would think it would work here since alco doesnt freeze unless its like whatever crazy amount below zero i believe. the only problem im thinking of right now is that 5% water. the water would frezze, but where, on top or below the alco? or maybe its even through out so the water will freeze in crystals and could be confusing, but they would melt at room temp. i really dont know anything about the science or chemistry of any of this, just from teks i read.
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mellowparty
legitimate researcher


Registered: 05/17/09
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Re: Does alcohol extraction work with mycelium? [Re: oh_you_know]
#11897757 - 01/25/10 11:47 AM (14 years, 24 days ago) |
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Here is my hypothetical tek. It looks nice on theory but im not sure about the efficiency.
Freeze the shrooms while wet. This will cause the ice crystals to shear the fungal cell walls thus facilitate extraction of the active compounds. Afterwards mash it all up and add some sort of an acid. The H+ will stick to lone electron pair of the nitrogen. This is will create a highly soluble salt of the compound. Filtrate. Use some base to remove the N bound protons. After that seems a bit blurry but probably the actives will precipitate. Separate. Enjoy
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PrimeTech
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Re: Does alcohol extraction work with mycelium? [Re: LogicaL Chaos]
#25745983 - 01/15/19 05:01 PM (5 years, 1 month ago) |
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Hi, Have you done this using brf jars? Is it the same process? Also I would like to know the ratio of needed materials, like how many cakes and amount of solvent is needed for a decent blend. Thx
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Astonish
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Re: Does alcohol extraction work with mycelium? [Re: PrimeTech]
#25745988 - 01/15/19 05:02 PM (5 years, 1 month ago) |
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Quote:
PrimeTech said: Hi, Have you done this using brf jars? Is it the same process? Also I would like to know the ratio of needed materials, like how many cakes and amount of solvent is needed for a decent blend. Thx
Bro this thread is 9 years old
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PrimeTech
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Re: Does alcohol extraction work with mycelium? [Re: PrimeTech]
#25746070 - 01/15/19 05:43 PM (5 years, 1 month ago) |
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Yes I'm aware.
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mocknbird
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Re: Does alcohol extraction work with mycelium? [Re: PrimeTech]
#27260469 - 03/19/21 01:27 PM (2 years, 10 months ago) |
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Quote:
PrimeTech said: Yes I'm aware.
Now that's what I call optimism!!
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