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Offlinericinus
suspicious indeed


Registered: 06/20/08
Posts: 90
Last seen: 6 years, 3 months
Spore syringes with charcoal - trouble creating an LC
    #11774896 - 01/06/10 04:39 AM (14 years, 1 month ago)

Hi everyone, I'm having problems creating an LC with strains that i have in spore syringes that have some charcoal added (Panaeolus cyanescens Cull and Psilocybe mexicana A). I make my LC from 4% solution of honey...any suggestions why the problem? Other strains without the charcoal are doing great...

TC,
ricinus

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Offlinefundamentalchair
Stand up
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Registered: 07/13/09
Posts: 801
Last seen: 12 years, 9 months
Re: Spore syringes with charcoal - trouble creating an LC [Re: ricinus]
    #11775130 - 01/06/10 07:03 AM (14 years, 1 month ago)

So you're saying that...

LC w/ charcoal doesn't work.
LC w/o charcoal does work.

Where's the problem? Quit putting in charcoal.

If you got a brew-it-your-self shop around, buy some light malt extract. 1% by weight (1g LME:99-100cc dH2O) works fantastically and is pretty clear once the myc ends up eating the nutrients.

Some people filter the LME before PC to get the sediment out, although I've never thought it much of a problem.


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Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually), :pm: me.

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Offlinericinus
suspicious indeed


Registered: 06/20/08
Posts: 90
Last seen: 6 years, 3 months
Re: Spore syringes with charcoal - trouble creating an LC [Re: fundamentalchair]
    #11775468 - 01/06/10 09:27 AM (14 years, 1 month ago)

I bought the spore syringes from a spore vendor, and these syringes have some charcoal added to the spore solution in order to minimize the contams..When I put some of these spores into the LC the mycelia doesn't seem to grow...I quite don't get it why it doesn't want to grow..In the LC the charcoal goes to the bottom of the vial and creates a sediment and there's no sign of mycelia..other strains that have no charcoal added to the spore solution are growing great in the LC so I suspected that the charcoal is the problem...


TC

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InvisibleDoc_T
Random Dude
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Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: Spore syringes with charcoal - trouble creating an LC [Re: ricinus]
    #11775522 - 01/06/10 09:38 AM (14 years, 1 month ago)

Grain LC.


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You make it all possible. Doesn't it feel good?

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OfflineWorkmanV
1999 Spore War Veteran
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Registered: 03/01/01 Happy 23rd Shroomiversary!
Posts: 3,598
Loc: Oregon, USA
Last seen: 4 hours, 53 minutes
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Re: Spore syringes with charcoal - trouble creating an LC [Re: ricinus]
    #11775700 - 01/06/10 10:14 AM (14 years, 1 month ago)

The charcoal must help with the microscopic viewing of the spores.  I can't think of any other reason for its presence without implying intent to cultivate.  Good luck!


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