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seamore
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Registered: 09/10/09
Posts: 68
Last seen: 14 years, 10 months
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need help with syringes
#11745760 - 12/30/09 10:17 PM (15 years, 20 days ago) |
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ok im new to mushroom cultivation. i was wondering what i do i have a mushroom print and trying to get it extracted into syringes but i dont know how. any help please?
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gus330
Stranger



Registered: 04/27/09
Posts: 2,609
Loc: south
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Re: need help with syringes [Re: seamore]
#11745849 - 12/30/09 10:31 PM (15 years, 20 days ago) |
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-------------------- favorite links
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Randomstickynote



Registered: 07/25/09
Posts: 824
Loc: TX
Last seen: 4 years, 2 months
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Re: need help with syringes [Re: seamore]
#11745876 - 12/30/09 10:37 PM (15 years, 20 days ago) |
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http://www.mushroomvideos.com/Sporeprinting-Syringes
That's one way. Theres tons of ways though. I just suck boiling water up into a syringe about four times, leaving the water in the last time. After that cools I load the syringe, one sterile petri and my inoculation loop in my glovebox. Open the print, scrape some spores into the petri, flame sterilize the syringe, shoot a drop or two away from the petri so it's not shooting boiling water at my spores, then drop a few drops over the spores I've scraped off. Suck that up, maybe squirt out a little more and suck it back in if I missed any and then it's done. If the print was more than a month or two old let it sit for a few hours or overnight to let the spores rehydrate and then use it!
EDIT:
Quote:
gus330 said: //www.shroomery.org/8403/Psilocybers-Syringe-Tek
Do that.
Edited by Randomstickynote (12/30/09 10:42 PM)
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thomasbe
biased!



Registered: 05/30/09
Posts: 174
Last seen: 11 years, 3 months
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thats efficieny right there
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seamore
Stranger


Registered: 09/10/09
Posts: 68
Last seen: 14 years, 10 months
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Re: need help with syringes [Re: thomasbe]
#11746006 - 12/30/09 11:07 PM (15 years, 20 days ago) |
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i have 70% alcohol will that work with it?
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Randomstickynote



Registered: 07/25/09
Posts: 824
Loc: TX
Last seen: 4 years, 2 months
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Re: need help with syringes [Re: seamore]
#11746012 - 12/30/09 11:09 PM (15 years, 20 days ago) |
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Yes
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gus330
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Registered: 04/27/09
Posts: 2,609
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Re: need help with syringes [Re: seamore]
#11746015 - 12/30/09 11:09 PM (15 years, 20 days ago) |
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yea that's what i use works great
-------------------- favorite links
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seamore
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Registered: 09/10/09
Posts: 68
Last seen: 14 years, 10 months
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Re: need help with syringes [Re: seamore]
#11746058 - 12/30/09 11:16 PM (15 years, 20 days ago) |
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ok i got all that do i wipe the rtv sealant with alcohol than use the inject the needle in it? than after that how do i seal the area that i stuck the needle in? do i put more rtv on the area? sorry for all the questions i just want to make sure i do this right the first time
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gus330
Stranger



Registered: 04/27/09
Posts: 2,609
Loc: south
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Re: need help with syringes [Re: seamore]
#11746100 - 12/30/09 11:20 PM (15 years, 20 days ago) |
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dude which tek are you doing so we can help you out a little more
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seamore
Stranger


Registered: 09/10/09
Posts: 68
Last seen: 14 years, 10 months
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Re: need help with syringes [Re: seamore]
#11746106 - 12/30/09 11:22 PM (15 years, 20 days ago) |
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not sure man.. i have a brother helping me but its long distance help so our schedules conflict. so im trying to get help from you guys..
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gus330
Stranger



Registered: 04/27/09
Posts: 2,609
Loc: south
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Re: need help with syringes [Re: seamore]
#11746132 - 12/30/09 11:27 PM (15 years, 20 days ago) |
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you need to read alot more on here I can,t help with every step man I had to read for months and ask questions about certain teks pick a tek and follow it all the way here is a link for noobies easy,easyhttp:///www.shroomery.org/10256/PF-Tek-v2
-------------------- favorite links
Edited by gus330 (12/30/09 11:29 PM)
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Randomstickynote



Registered: 07/25/09
Posts: 824
Loc: TX
Last seen: 4 years, 2 months
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Re: need help with syringes [Re: seamore]
#11746143 - 12/30/09 11:30 PM (15 years, 20 days ago) |
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I'm assuming you're talking about a jar of sterile water here? With an RTV injection port?
In that case, yes, wipe it down with alcohol, pretty much wipe everything down with alcohol except your spores and whatever you're putting them in before you suck them up into your syringe. If you use a needle or a loop to scrape off the spores you can flame sterilize that and cool it (so it doesn't burn your spores) by lightly touching it to a paper towel soaked in alcohol, but don't let the loop/syringe get soaked in alcohol, or at least let it dry before you touch your spores with it. A sterilized shot glass will work fine but I just use petris because I have them and they're pre-sterilized.
If you must wipe the shot glass down with alcohol before putting your spores in it (like if you can't wrap the shot glass in foil and pressure cook it to sterilize it) then let it completely dry before you scrape your spores into it, you don't want to scrape your spores into a pool or even a tiny film of alcohol.
As for the area that you stuck the needle in, if it's a really thick needle (like 16-14 gauge) then it might make a hole, but usually RTV ports seal themselves if it's the first time you've injected through them. If these ports are on your grain jars or your PF jars and your worried about anything getting in there's no harm in putting some tape over the port just in case it didn't seal back right.
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