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Offlinefundamentalchair
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LC: LME vs. LME + Dextrose
    #11743960 - 12/30/09 05:03 PM (14 years, 1 month ago)

I've been wondering about the necessity of adding dextrose to a LC medium. Looking at FastFred's Media Cookbook It shows this simple agar recipie.

Quote:

Malt Extract Agar (3%)(MEA)(G]eneral Microbiology)(FDA M93)

    30 g Malt extract
    20 g Agar
    1 L Distilled water

    Boil to dissolve ingredients. Avoid overheating, which causes softening of agar and darkening of medium color. Autoclave 15 min at 121°C. Dispense 20-25 ml into sterile 15 x 100 mm petri dishes. Final pH, 5.5 ± 0.2.

    This medium is recommended as a general maintenance medium.




While I can't find the tek, I seem to remember one making LC with 1% LME mix in water without any other additions. I can't seem to find the tek (or any other discussions relating to LME vs. LME + Dextrose).

What is the important of adding dextrose to LC yet leaving it out of a dish? I also seem to remember something about someone claiming the 'shock' from going to an exceptionally food abundant medium (dextrose LC) to a less food abundant medium (grain/brf) causing a 'slow start' so to say.

Are my memories founded in reality, or am I just having an episode again?


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Sclerotia FAQ... If ya ain't got any stones, grow some.

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Re: LC: LME vs. LME + Dextrose [Re: fundamentalchair]
    #11744030 - 12/30/09 05:15 PM (14 years, 1 month ago)

I can't really say, I've always used dextrose as a base, but with malt or other things added to it. Never just malt.
Why not try one of each? Adjust the amounts so it's even.


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Offlinefundamentalchair
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Re: LC: LME vs. LME + Dextrose [Re: Doc_T]
    #11744196 - 12/30/09 05:41 PM (14 years, 1 month ago)

Quote:

Doc_T said:
I can't really say, I've always used dextrose as a base, but with malt or other things added to it. Never just malt.
Why not try one of each? Adjust the amounts so it's even.




As of the moment, time and the lack of understanding of how to approach a control group for a comparative study between the 2. Oh yeah, and space.

For an experiment:

Grain culture for an innoculant (To keep down contamination or dilution of ingredients)

Differing ratios of media between a static water/media ratio.
  eg: 2 (or more) each of
      100% LME
      25% LME + 75% Dextrose
      50% LME + 50% Dextrose
      25% LME + 75% Dextrose
                100% Dextrose
      All at 10:1 water/media ratio. all filtered to remove sediment. All measured (mass) and recorded.

All noced with the same amount of grain culture. Measure colonization speed of LC. If many jars are possible, periodically remove one random of each of the above, filter and wash with dH20. Weigh resulting solids for total myc growth over time. Divide by jar's (before) liquid mass to get a growth per mass ratio of each, preferably plotted over time.

Run several (5?) jars from each into a grain spawn (again with measurement of weight of spawn) to get a time to colonize/mass of each.

For the control, would I then take a setup (for the first part) and leave some non innoced LC to then measure non water solulable sediment as a control group?

For colonization speed, would I run with MS inoc from the same syringe that started the grain spawn or would just nocking them up with the 'inert' culture be sufficient?

If done to the full extent, doing a wash/measure every week for 4 weeks, it would require...

6+ LC jars per media (week 1-4 +noc +grain)
x2 (noced and unnoced)

It would at a minimum take 60 jars, and that's only at a 25% seperation and one grain jar for each.

A better formula would be:

(((100 / sepperation % + 1) x number of time plots + 1 ) + Grain jars per LC jar) x 2

So, with a test at every 10% interval with 5 jars per LC, taken once a week for 6 weeks, it would require a total of 164 jars. That would be a completely respectable experiment.

:feelsbadman:


--------------------
semper necessitas probandi incumbit ei qui agit


Sclerotia FAQ... If ya ain't got any stones, grow some.

Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually), :pm: me.

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Re: LC: LME vs. LME + Dextrose [Re: fundamentalchair]
    #11744205 - 12/30/09 05:43 PM (14 years, 1 month ago)

I was thinking just one jar of one and one of the other, and see if you even notice a difference.


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Offlinefundamentalchair
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Re: LC: LME vs. LME + Dextrose [Re: Doc_T]
    #11744286 - 12/30/09 06:00 PM (14 years, 1 month ago)

Quote:

Doc_T said:
I was thinking just one jar of one and one of the other, and see if you even notice a difference.




You're no fun.


--------------------
semper necessitas probandi incumbit ei qui agit


Sclerotia FAQ... If ya ain't got any stones, grow some.

Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually), :pm: me.

My Trade list.

Ghetto Tek: Auto FAE & Light


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Offlinefungus_tao
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Re: LC: LME vs. LME + Dextrose [Re: fundamentalchair]
    #11744317 - 12/30/09 06:06 PM (14 years, 1 month ago)

I have used just LME for liquid culture several times with edibles. Just eyeballed it too. my LC turned out great. About a tsp to a pint. my hypothesis both would work well on their own, and mixing the two is pointless.


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The Light is your guide.


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Re: LC: LME vs. LME + Dextrose [Re: fundamentalchair]
    #11744345 - 12/30/09 06:11 PM (14 years, 1 month ago)

Quote:

fundamentalchair said:
Quote:

Doc_T said:
I was thinking just one jar of one and one of the other, and see if you even notice a difference.




You're no fun.




Oh, I'm fun. I've always wondered how a dextrose-only LC is even possible, maybe you should do one of those and weigh it like you were saying.


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Offlinefundamentalchair
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Re: LC: LME vs. LME + Dextrose [Re: Doc_T]
    #11744409 - 12/30/09 06:21 PM (14 years, 1 month ago)

Quote:

Oh, I'm fun.




Alright Doc. You, me, and Boxxy. what end do you want?

Quote:

I've always wondered how a dextrose-only LC is even possible




Extremly malnourished mycellium? :shrug:

I have some karo LC that turned out like poo compared to the straight LME LC.

Your dextrose/LME seems to come out clear, where my LME only is still always beer yellow. I didn't strain either, so that could be a huge contributing factor. I like having 15g needles that'll suck up a kidney stone.


--------------------
semper necessitas probandi incumbit ei qui agit


Sclerotia FAQ... If ya ain't got any stones, grow some.

Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually), :pm: me.

My Trade list.

Ghetto Tek: Auto FAE & Light


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Re: LC: LME vs. LME + Dextrose [Re: fundamentalchair]
    #11744558 - 12/30/09 06:48 PM (14 years, 1 month ago)

Clear? No way. Cloudy beyond measure, I don't usually filter it.
It clarifies as the myc eats the nutrients.


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Offlinefundamentalchair
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Re: LC: LME vs. LME + Dextrose [Re: Doc_T]
    #11744649 - 12/30/09 07:10 PM (14 years, 1 month ago)

Quote:

Doc_T said:
Clear? No way. Cloudy beyond measure, I don't usually filter it.
It clarifies as the myc eats the nutrients.




I mean, it doesn't look like a brewski with floaties (or jellyfish in my case) like this.



I got $10 on me getting hammered some night and making some LC out of some bud light.


--------------------
semper necessitas probandi incumbit ei qui agit


Sclerotia FAQ... If ya ain't got any stones, grow some.

Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually), :pm: me.

My Trade list.

Ghetto Tek: Auto FAE & Light


Extras: Filter Print Post Top
InvisibleDoc_T
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Re: LC: LME vs. LME + Dextrose [Re: fundamentalchair]
    #11744666 - 12/30/09 07:13 PM (14 years, 1 month ago)

No it ends up like this:


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