Home | Community | Message Board

MushroomMan Mycology
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblegus330
Stranger
Male


Registered: 04/27/09
Posts: 2,609
Loc: south Flag
liquid culture
    #11743103 - 12/30/09 02:40 PM (15 years, 21 days ago)

is it necessary to have two holes in your lid or just one self healing injection port when preparing an lc


--------------------
favorite links

Edited by gus330 (12/30/09 02:42 PM)

Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: liquid culture [Re: gus330]
    #11743121 - 12/30/09 02:44 PM (15 years, 21 days ago)

It's a lot simpler to get the liquid out of the jar if there's a breather hole. Easier still if you have a  needleless connector port too.


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
Invisiblegus330
Stranger
Male


Registered: 04/27/09
Posts: 2,609
Loc: south Flag
Re: liquid culture [Re: Doc_T]
    #11743147 - 12/30/09 02:47 PM (15 years, 21 days ago)

could you use micro pore tape on a smaller hole


--------------------
favorite links

Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: liquid culture [Re: gus330]
    #11743175 - 12/30/09 02:52 PM (15 years, 21 days ago)

Could you? Yes. I've used polyfil, too. Is there a risk of wetting it if you aren't careful enough? Yes. So be careful.


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
Invisiblegus330
Stranger
Male


Registered: 04/27/09
Posts: 2,609
Loc: south Flag
Re: liquid culture [Re: Doc_T]
    #11743207 - 12/30/09 02:59 PM (15 years, 21 days ago)

I have plenty off polyfill how big of a hole do you put when using poly fil


--------------------
favorite links

Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: liquid culture [Re: gus330]
    #11743227 - 12/30/09 03:03 PM (15 years, 21 days ago)

I just punch a hole with a screwdriver. 1/4" or so, I guess. Like in the link I gave upthread.


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
OfflineSillyBilly
Professional

Registered: 03/05/09
Posts: 3,634
Loc: Californi-eh
Last seen: 9 years, 29 days
Re: liquid culture [Re: Doc_T]
    #11743270 - 12/30/09 03:10 PM (15 years, 21 days ago)

I don't use a breathing hole in mine anymore.
It's not absolutely necessary as I have heard pertaining to lc's.


--------------------
By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din

Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: liquid culture [Re: SillyBilly]
    #11743306 - 12/30/09 03:18 PM (15 years, 21 days ago)

How do you equalize the pressure? Do your LC jars ever suck in an entire spore syringe?


--------------------
You make it all possible. Doesn't it feel good?

Extras: Filter Print Post Top
OfflineSillyBilly
Professional

Registered: 03/05/09
Posts: 3,634
Loc: Californi-eh
Last seen: 9 years, 29 days
Re: liquid culture [Re: Doc_T]
    #11743356 - 12/30/09 03:25 PM (15 years, 21 days ago)

I pc them with the lid a little loose and cover them with tin foil.
I don't think I have ever ended up with a great addition or subtraction of water.


--------------------
By the livin' Gawd that made you,
    You're a better man than I am, Gunga Din

Extras: Filter Print Post Top
Invisiblemyceleus_rex
seeker
Male User Gallery


Registered: 07/01/09
Posts: 1,581
Loc: alized
Re: liquid culture [Re: SillyBilly]
    #11743832 - 12/30/09 04:39 PM (15 years, 21 days ago)

I've done some grain LC'c recently with two self-healing ports, one of which I use with a polyfil stuffed syringe to equalize pressure. Works great so far and for me, seems like the way to go.


--------------------
Cervantes' sclerotia thread    Citric's self-healing lid tek  Agar's Grain LC  Breaking Up Spawn Jars

World's Ugliest Cat?

If ignorance is bliss, why aren't there more happy people?

Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Myyco.com Golden Teacher Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   North Spore Injection Grain Bag   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Spores, G2G, Liquid Culture, Cloning & Colonization Time.
( 1 2 3 all )
agar 54,401 43 07/08/23 07:04 AM
by Screwup
* Liquid Culture Challenges Leimana 9,819 4 05/31/05 11:33 PM
by Corporal Kielbasa
* How to make a liquid cultures FAST (like two days)!
( 1 2 3 4 all )
Blue Helix 41,847 68 05/26/13 05:12 PM
by Jman420
* 1st Liquid Culture, arrrgh! Seril 14,929 10 02/28/06 08:35 PM
by Limelight_Liver
* Liquid Culture Recipes MrMaddHatter 24,556 13 03/29/05 04:53 AM
by Sbealzebub
* how to use cardboard cultures to inoculate grain jars and how to use a liquid culture to inoculate lordoftheshroomz 6,663 12 05/08/05 03:55 PM
by lordoftheshroomz
* Clear liquid cultures ? mickywilliams2 3,856 11 10/12/05 05:02 PM
by Bamaman
* i can get liquid cultures, but not grain laughingbuddha 2,909 8 03/08/05 12:02 PM
by laughingbuddha

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
316 topic views. 35 members, 150 guests and 109 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2025 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.007 seconds on 12 queries.