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Invisiblemango man
crazed!
Male

Registered: 02/02/09
Posts: 344
Loc: north CUNTry Flag
Where do most of you do your agar work?
    #11739833 - 12/29/09 08:47 PM (15 years, 21 days ago)

I want to start doing some projects with agar but I don't have the means to construct a flowhood.
I was wondering if you all do your agar work in front of a flowhood or in a glovebox?
I don't see the point in wasteing my time starting these projects in a glovebox if they are most likely going to fail.
Sorry for the noob question.
MM


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Always interested in trading with trusted Shroomerites!
I'm looking for edibles and prints from woodlovers.
:pm: me

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InvisibleDoc_T
Random Dude
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Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: Where do most of you do your agar work? [Re: mango man]
    #11739852 - 12/29/09 08:50 PM (15 years, 21 days ago)

With good technique, a glovebox will work fine. And a flowhood won't save you from poor technique.


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You make it all possible. Doesn't it feel good?

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OfflineThyrax
No way
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Registered: 01/03/08
Posts: 1,068
Loc: Montreal, Quebec
Last seen: 14 years, 4 months
Re: Where do most of you do your agar work? [Re: mango man]
    #11739863 - 12/29/09 08:52 PM (15 years, 21 days ago)

You will have less succes with a GB but if you sterile procedure are good you should have pretty good result. I just don't like to do agar in a glove box but thats me.


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OfflineThyrax
No way
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Registered: 01/03/08
Posts: 1,068
Loc: Montreal, Quebec
Last seen: 14 years, 4 months
Re: Where do most of you do your agar work? [Re: Doc_T]
    #11739866 - 12/29/09 08:53 PM (15 years, 21 days ago)

Quote:

Doc_T said:
And a flowhood won't save you from poor technique.


:super:


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Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: Where do most of you do your agar work? [Re: Thyrax]
    #11739918 - 12/29/09 09:04 PM (15 years, 21 days ago)

I have had great success w/ a clear rubbermaid w/ 2 holes in it. I just set it on a little table in the living room and work in front of the boob tube.


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The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

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Invisiblemango man
crazed!
Male

Registered: 02/02/09
Posts: 344
Loc: north CUNTry Flag
Re: Where do most of you do your agar work? [Re: anonjon]
    #11739936 - 12/29/09 09:07 PM (15 years, 21 days ago)

I think my sterile technique is good. I very rarely get any contam.
Guess this is a good way for me to test myself and my technique.
MM


--------------------
Always interested in trading with trusted Shroomerites!
I'm looking for edibles and prints from woodlovers.
:pm: me

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Invisiblecurbstop
Regnarts


Registered: 06/02/09
Posts: 810
Re: Where do most of you do your agar work? [Re: mango man]
    #11739980 - 12/29/09 09:15 PM (15 years, 21 days ago)

I too have a Question in which the OP will benefit from, Is agar more sensitive to contams then say PF TEK and or Grain work?

Reason I am asking this is like the OP said,I very very Rarley have a contam but have yet to work on agar for the fact that I was under the impression that agar was extremely  sensitive to contams.


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is multispore a spore company

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InvisibleCitizen13
Jack's wasted life.


Registered: 11/01/09
Posts: 1,319
Re: Where do most of you do your agar work? [Re: curbstop]
    #11740003 - 12/29/09 09:19 PM (15 years, 21 days ago)

Quote:

Is agar more sensitive to contams then say PF TEK and or Grain work?




think of agar as a substrate on steroids.
:stoned:


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OfflineRandomstickynote
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Registered: 07/25/09
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Re: Where do most of you do your agar work? [Re: curbstop]
    #11740041 - 12/29/09 09:24 PM (15 years, 21 days ago)

It's not extremely sensitive. I've had a stack of agar plates sitting in the back of my glovebox uncovered, with no cover over the arm holes for 36 hours before. Came back to the stack after running errands, living life, etc, inoculated and didn't get a single contam due to it sitting.

The reason it's more sensitive is that you have to open them and expose the entire usable surface area to the elements as opposed to injecting in a tiny hole creating a minuscule opportunity for contamination. Also they don't seal air tight like jars, but if you wrap in press n' seal or parafilm that shouldn't be an issue.


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AMU

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OfflineThyrax
No way
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Registered: 01/03/08
Posts: 1,068
Loc: Montreal, Quebec
Last seen: 14 years, 4 months
Re: Where do most of you do your agar work? [Re: Randomstickynote]
    #11740140 - 12/29/09 09:41 PM (15 years, 21 days ago)

I don't see why mold spore would germinate more or less on agar. They will probably grow faster but who care. I may be wrong but im pretty sure its the same same thing as the other substrate. The difference is in the way to do it, There is a lot more chance that you intoduce contam in your agar while doing it cus of the way you do it.


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Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: Where do most of you do your agar work? [Re: Randomstickynote]
    #11741615 - 12/30/09 05:41 AM (15 years, 21 days ago)

Quote:

Randomstickynote said: Also they don't seal air tight like jars,




they do if you use jars for your agar.


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The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

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Offlinesugarcane
Lunatic


Registered: 01/16/09 Happy 16th Shroomiversary!
Posts: 29
Last seen: 14 years, 10 months
Re: Where do most of you do your agar work? [Re: anonjon]
    #11741835 - 12/30/09 10:40 AM (15 years, 21 days ago)

My FOAF does his in any room without a draft. Meaning anywhere, just close the windows and make sure the air is not moving.

Barely gets anything contaminated.

He does wear gloves and a mask and sanitizes with alcohol and of course flame sterilizes with every move.

For the non-professional, he really doesnt think flow hoods or gloveboxes are necessary. When the time comes for you to build or buy one, you will know.

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Invisibleanonjon
Partially Right

Registered: 11/03/08
Posts: 6,322
Re: Where do most of you do your agar work? [Re: sugarcane]
    #11742092 - 12/30/09 11:36 AM (15 years, 21 days ago)

Quote:

sugarcane said:

For the non-professional, he really doesnt think flow hoods or gloveboxes are necessary. When the time comes for you to build or buy one, you will know.




what is that supposed to mean. Amateur hobbyists won't benefit from a 7 dollar glove box?


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The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

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OfflineThyrax
No way
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Registered: 01/03/08
Posts: 1,068
Loc: Montreal, Quebec
Last seen: 14 years, 4 months
Re: Where do most of you do your agar work? [Re: anonjon]
    #11742116 - 12/30/09 11:41 AM (15 years, 21 days ago)

Quote:

anonjon said:
Quote:

sugarcane said:

For the non-professional, he really doesnt think flow hoods or gloveboxes are necessary. When the time comes for you to build or buy one, you will know.




what is that supposed to mean. Amateur hobbyists won't benefit from a 7 dollar glove box?



yeah a glove box is so cheap that no one should not use one ...


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Invisiblenoobieshroomie
Back again
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Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure Flag
Re: Where do most of you do your agar work? [Re: anonjon]
    #11742117 - 12/30/09 11:41 AM (15 years, 21 days ago)

glove box at least
in the last month i have done almost 100 dishes
i have lost 4, 3 were bad cultures to start with
and one was my fault


      -noobie-


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AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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Offlinesugarcane
Lunatic


Registered: 01/16/09 Happy 16th Shroomiversary!
Posts: 29
Last seen: 14 years, 10 months
Re: Where do most of you do your agar work? [Re: noobieshroomie]
    #11744411 - 12/30/09 06:22 PM (15 years, 21 days ago)

How about this...

get the agar and the dishes, work with them w/o a box or a hood.

see what you get.

if it contams, then get one or the other, but my bet is you wont need to.

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Invisiblemango man
crazed!
Male

Registered: 02/02/09
Posts: 344
Loc: north CUNTry Flag
Re: Where do most of you do your agar work? [Re: sugarcane]
    #11744462 - 12/30/09 06:30 PM (15 years, 21 days ago)

I actually placed an order today and will have my dishes very soon.
I plan on useing my glovebox. It seems that this whole project is going to be far cheaper than I had origionally anticipated anyway.
Thank you all for the info as always.
MM


--------------------
Always interested in trading with trusted Shroomerites!
I'm looking for edibles and prints from woodlovers.
:pm: me

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InvisibleDanKnugget
Mysterious Traveller
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Registered: 07/15/03
Posts: 1,074
Loc: On a boat
Re: Where do most of you do your agar work? [Re: mango man]
    #11744549 - 12/30/09 06:47 PM (15 years, 21 days ago)

I hate working in a glovebox, I find it very akward and uncomfortable.  But then again i made my glovebox like a retard.  I put the holes on the 2 sides so I am hugging it whenever i use it.  Needless to say i try to use it as little as possible.  I will be making a new one one of these days, and I would recommend putting the holes on the same side, like noobieshroomie's happy glovebox :smile:


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My 3 proverbs:
1. School is for people who don't know stuff.
2. Jobs are for losers.
3. Reading is for people with nothing better to do.

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InvisibleCitizen13
Jack's wasted life.


Registered: 11/01/09
Posts: 1,319
Re: Where do most of you do your agar work? [Re: DanKnugget]
    #11744571 - 12/30/09 06:50 PM (15 years, 21 days ago)

wtf why would you do that:lol:

you must look like an idiot while using it (no offense)


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InvisibleDanKnugget
Mysterious Traveller
Male


Registered: 07/15/03
Posts: 1,074
Loc: On a boat
Re: Where do most of you do your agar work? [Re: Citizen13]
    #11744648 - 12/30/09 07:10 PM (15 years, 21 days ago)

yes, i feel like an idiot too :blush: Also, the arm holes are a bit small and its a pain to squeeze my hands through.  Don't build you glove box stoned :P I put them like that so i could reach every point in the box, i think...


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My 3 proverbs:
1. School is for people who don't know stuff.
2. Jobs are for losers.
3. Reading is for people with nothing better to do.

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