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Pajarito
Three Little Birds


Registered: 12/16/09
Posts: 190
Loc: Yuggoth
Last seen: 11 months, 21 days
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Pollock, DFA and fruiting on agar
#11717683 - 12/25/09 05:02 PM (14 years, 1 month ago) |
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Many years ago I experimented with Dog Food Agar (DFA) after obtaining Steven Pollock's seminal work, Magic Mushroom Cultivation. DFA was a big change from the standard MEA and PDA that I was familiar with, and I found that cubensis would rapidly fruit from multi-spore inoculation on DFA and was a quick and reliable way to establish good fruiting strains by cloning the best fruits from the agar dishes.
Now that I have become active in this hobby again, I have been curious if this technique could also be applied to other tropical species besides cubensis, specifically the Mexicanae section. Has anyone had success in fruiting mexicana, tampanensis, ATL#7 or any other exoctic species on agar?
I was thinking about trying an experiment along these lines using straight DFA, DFA with a small amount of whole rye grass, and DFA with ground rye grass seed powder. Has anyone done any experimentation with these methods?
-------------------- AmScope T490 - OMAX 18MP USB3 camera - Toupview software - FP Series I "I suddenly became strangely inebriated." — Dr. Albert Hoffman "When you say the names of the plants, you say the names of the gods." — Dr. Timothy Plowman "No weak heart shall prosper." — Hon. Robert Nesta Marley
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Jitsu
JKD Love



Registered: 11/12/08
Posts: 1,073
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Re: Pollock, DFA and fruiting on agar [Re: Pajarito]
#11718625 - 12/25/09 09:38 PM (14 years, 1 month ago) |
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Mushrooms can fruit from any kind of medium.
I have tried experiments with cloning fruit bodies from all different types of agar. All my results came out very poor as opposed to a diverse substrate with a good moisture content.
As far as your question goes... Are you curious about sclerotia formations on agar or the mushroom fruit body?
-------------------- Do not deny the classical approach, simply as a reaction, or you will have created another pattern and trapped yourself there. How I get my Pinsets The Capabilities Of A Shotgun FC
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Pajarito
Three Little Birds



Registered: 12/16/09
Posts: 190
Loc: Yuggoth
Last seen: 11 months, 21 days
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Re: Pollock, DFA and fruiting on agar [Re: Jitsu]
#11719002 - 12/25/09 11:06 PM (14 years, 1 month ago) |
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With this method, I am interested in fruit bodies. My only experience in using this technique has been cloning the most robust specimens of cubensis fruiting from the DFA cultures, and was never disappointed when these isolates wher fruited on cased grain and bulk substrates, It seemed to be a huge shortcut from isolating and growing out multiple sectors from dishes and then experimenting with them individually in subsequent inoculations to substrate to isolate promising strains.
Back in those days I didn't have access to the more exotic species to experiment, with except from the prints I obtained from Steven Peele's FMRC, unfortunately non of which I had any luck in culturing. Homestead, Syzygy and PES were always reliable back then, but of course they were all cubes!
Perhaps my successes were just luck, perhaps not. But after previously spending the time to isolate some good fruiting isolates with trial and effort, I was really impressed how well DFA worked to provided extremely quick cubensis fruitings. I would use smaller petri dishes that would allow me to place inverted wide mouth jars over them to allow plenty of room and the environment for the mushrooms to form normally.
I know that one can obtain sporadic fruitings of fruit bodies on typical agar, but in my experience nothing compared to DFA. Go Purina! I think it is worth the experimentation to work with the exotic species, although I think they may require additional additives to the agar depending on species, and see if I can find any worthwhile success in this method. I'll share any findings in the coming weeks.
-------------------- AmScope T490 - OMAX 18MP USB3 camera - Toupview software - FP Series I "I suddenly became strangely inebriated." — Dr. Albert Hoffman "When you say the names of the plants, you say the names of the gods." — Dr. Timothy Plowman "No weak heart shall prosper." — Hon. Robert Nesta Marley
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Jitsu
JKD Love



Registered: 11/12/08
Posts: 1,073
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Re: Pollock, DFA and fruiting on agar [Re: Pajarito]
#11723066 - 12/26/09 08:21 PM (14 years, 1 month ago) |
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Your performance might be low but you probably won't have trouble obtaining a few fruits.
By chance are you going to experiment with edible or medicinal mushrooms in this manner or are you sticking strictly to the Psilocybes? I'd be interested in seeing the outcome of a cloned culture grown on agar when applied to a bulk substrate.
-------------------- Do not deny the classical approach, simply as a reaction, or you will have created another pattern and trapped yourself there. How I get my Pinsets The Capabilities Of A Shotgun FC
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Pajarito
Three Little Birds



Registered: 12/16/09
Posts: 190
Loc: Yuggoth
Last seen: 11 months, 21 days
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Re: Pollock, DFA and fruiting on agar [Re: Jitsu]
#11738733 - 12/29/09 05:46 PM (14 years, 1 month ago) |
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I'm not sure if many people experiment with Dog Food Agar these days, but I highly recommend it. As I said above, I originally read about this formula in Pollock's book, and once I tried it and was amazed by how well DFA would fruit cubensis, it was my main tek for rapidly isolating fruiting strains.
On a side note, Pollock turned me onto the tek of using pure brown rice cakes as a substrate. That was many years ago, but I can definitely say that my friends were more impressed with spent brown rice cakes than they were with fruit-bodies. After a few flushes, the brown rice cake would shrink to the point to where you could just flip it right out of a narrow mouth mason jar. One spent rice cake, crumbled up and boiled into a tea was enough to send to blast 4 psychonauts to pluto and and beyond.
In my pantheon, Pollock is right up there with Schultes, McKenna and other entheo-saints 
I am starting my DFA experiments with exotics (Panaeolus and Mexicianae) this week.
-------------------- AmScope T490 - OMAX 18MP USB3 camera - Toupview software - FP Series I "I suddenly became strangely inebriated." — Dr. Albert Hoffman "When you say the names of the plants, you say the names of the gods." — Dr. Timothy Plowman "No weak heart shall prosper." — Hon. Robert Nesta Marley
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Doc_T
Random Dude




Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Pollock, DFA and fruiting on agar [Re: Pajarito]
#11738930 - 12/29/09 06:18 PM (14 years, 1 month ago) |
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Super- be sure to keep us posted!
-------------------- You make it all possible. Doesn't it feel good?
Edited by Doc_T (12/29/09 06:36 PM)
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