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OfflineVespertineDweller
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First Time Grow Log And Queries (pics)
    #11508976 - 11/23/09 04:06 AM (15 years, 1 month ago)

Hello Everyone,

First Time poster

So i've been poring through this website for the last couple of weeks. Paul Stamets TED talk was what originally got me intrigued in the world of mycology. I suggest you go check it out it's well a doozy and we can talk about it in some other forum i guess huh.

http://www.ted.com/talks/lang/eng/paul_stamets_on_6_ways_mushrooms_can_save_the_world.html

This is what i'm working with. I got beginners itch and started hastily, going down to a local seed bank and purchasing a spore syringe. The person working the shop knew nothing more than it was a cubensis strain, though not the particulars. You'd think $30 is worth knowing what's growing but anyhow 9 days later (inoculated 5 PF tek brown rice flour/vermiculite jars on friday the 13th) and this is where I stand, or sit, or crouch or whatever...



5th Jar is looking much the same as the others.

5/5 no contams i'm happy.

As I mentioned earlier I got beginners itch but since rooting around a bunch on here I think i want to switch away from just a basic PF tek situation. I'm now thinking of doing 2 cakes in the typical perlite PF tek fashion, Casing 2 cakes in some sort of chamber, and with the 3rd cut out a wedge a la...: (See: http://www.shroomery.org/67/Poor-Mans-Mycelium-Syringe-Tek
... to make syringes to inoculate the popcorn i bought tonight for a bulk substrate. (see: http://www.shroomery.org/9033/Popcorn-Grain-Spawn
and for real in depth reading about it see: http://www.shroomery.org/forums/showflat.php?fpart=all&Board=Forum4&Number=1152356&fpart=all)

As my initial PF jars are only what like 30% finished i'm looking for guidance on my experimentation. I'm also wondering if the poor mans syringe tek is a viable option for innoculating popcorn that i will be Tyndallizing (see: http://www.shroomery.org/5248/What-is-fractional-sterilization-or-tyndallization). Also would it be beneficial to combine the poor mans syringe tek with the honey tek (see: http://www.shroomery.org/66/Club99s-Honeywater-Mycelium-Tek).


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"By engaging mycelium we can change the world" -Paul Stamets

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InvisibleMr.gym
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Re: First Time Grow Log And Queries (pics) [Re: VespertineDweller]
    #11508986 - 11/23/09 04:19 AM (15 years, 1 month ago)

The one on the far left of the first pic looks like it has some trich near the inoc. point. Can you get a closeup of the mycelium of that one?

Welcome to the shroomery!


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OfflineLancaster
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Re: First Time Grow Log And Queries (pics) [Re: Mr.gym]
    #11509031 - 11/23/09 04:47 AM (15 years, 1 month ago)

You should certainly do a liquid culture if you have anything remaining in that syringe. This tek is beginner friendly. You'll be able to inoculate dozens of jars from a few CCs of spore solution.


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OfflineVespertineDweller
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Re: First Time Grow Log And Queries (pics) [Re: Lancaster]
    #11509038 - 11/23/09 04:55 AM (15 years, 1 month ago)

Alas, not a drop left:frown:


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"By engaging mycelium we can change the world" -Paul Stamets

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OfflineLancaster
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Re: First Time Grow Log And Queries (pics) [Re: VespertineDweller]
    #11509627 - 11/23/09 09:32 AM (15 years, 1 month ago)

Quote:

VespertineDweller said:
Alas, not a drop left:(



Well you can always make a print or tissue sample from your current grow once it's finished.  :super:


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OfflineVespertineDweller
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Re: First Time Grow Log And Queries (pics) [Re: Lancaster]
    #11521385 - 11/24/09 06:58 PM (15 years, 1 month ago)

So can I make a liquid culture like this

http://www.shroomery.org/forums/showflat.php/Number/6194327#6194327

using my PF jars once they are fully colonized by cutting off a piece, putting it in a jar i've sterilized with water, honey, coffee, gypsum and a couple shards of glass?


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"By engaging mycelium we can change the world" -Paul Stamets

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OfflineLancaster
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Re: First Time Grow Log And Queries (pics) [Re: VespertineDweller]
    #11521981 - 11/24/09 08:13 PM (15 years, 1 month ago)

Quote:

VespertineDweller said:
So can I make a liquid culture like this

http://www.shroomery.org/forums/showflat.php/Number/6194327#6194327

using my PF jars once they are fully colonized by cutting off a piece, putting it in a jar i've sterilized with water, honey, coffee, gypsum and a couple shards of glass?



You certainly could, you would be cloning, but you may run into problems with sterilization. You'd probably be better off waiting for your grow to fruit, selecting the largest/fastest growing specimen, and cloning that using this tek.


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OfflineVespertineDweller
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Re: First Time Grow Log And Queries (pics) [Re: Lancaster]
    #11527461 - 11/25/09 04:44 PM (15 years, 1 month ago)

oh man i'm a boner and somehow just managed to find the mushrooms cultivation archive posted right up there at the top. OOPS. My bad. Not only am i a noob grower but i havent used a forum like this since i was probably 14.

Anyways here's the progress. Seems kind of slow compared to what some folks on here show. Should I be concerned?



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"By engaging mycelium we can change the world" -Paul Stamets

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OfflineVespertineDweller
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Re: First Time Grow Log And Queries (pics) [Re: VespertineDweller]
    #11690496 - 12/21/09 03:30 AM (15 years, 30 days ago)

Man its been a long time... Over a month and a week now. First pins are happening but... I came down with a case of trich and possibly some unidentified slime thing. I've trolled around plenty looking for pictures of something similar but couldn't find anything. It's basically a slime thats a yellowish brown, very similar to the color of my substrate just more liquified. I've kept the trich jar aside just to see what happens and the myc seems to be winning the battle

Can the mycelium take over the trich and be safe to birth and fruit (not in my house obviously)?

From these pictures what do you think the slime is?








Also if anyone feels like clearing up what i'm meant to do about these little pins before I dunk em that would rule. Do i gotta sterilize the water first and what temp should it be at?


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"By engaging mycelium we can change the world" -Paul Stamets

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OfflineVespertineDweller
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Re: First Time Grow Log And Queries (pics) [Re: VespertineDweller]
    #11692172 - 12/21/09 01:13 PM (15 years, 30 days ago)

sorry for the.... bump


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"By engaging mycelium we can change the world" -Paul Stamets

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OfflineEgo Questio
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Re: First Time Grow Log And Queries (pics) [Re: VespertineDweller]
    #11692209 - 12/21/09 01:24 PM (15 years, 30 days ago)

Best advice when you've got invitro contams is chuck it,Im afraid.
If contams develop during fruiting,you are sometimes able to deal with,but invitro it's just going to get worse.And when you open that jar,you're spreading spores everywhere.
PC it and dump it.

As for the slime,I can't really make it out. (on my phone)
if it's really bad,could be wet spot,but from the sounds of it,that's just the beautifully named mycellium piss. :grin:
that's all good.

But the trich is not. :sad:


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OfflineVespertineDweller
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Re: First Time Grow Log And Queries (pics) [Re: Ego Questio]
    #11692782 - 12/21/09 02:55 PM (15 years, 30 days ago)

if it's mushroom piss can I expect the mycelium to eventually colonize it's pissy pants?


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"By engaging mycelium we can change the world" -Paul Stamets

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OfflineBreakfast Crew
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Re: First Time Grow Log And Queries (pics) [Re: VespertineDweller]
    #11692813 - 12/21/09 02:58 PM (15 years, 30 days ago)

Its not metabolites, that is sour rot. If it smells like dirty gym socks then toss it out. Try using less water in your next batch.

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OfflineEgo Questio
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Re: First Time Grow Log And Queries (pics) [Re: VespertineDweller]
    #11692818 - 12/21/09 02:59 PM (15 years, 30 days ago)

I don't think so buddy.
You'll definately want to consult more experienced minds than mine before destroying your jar,but I'm fairly sure contam in a jar is a dead jar,
not sure why no one else is replying-maybe try PMig one of the noob helping groups,feelfamily or AMU?


--------------------
Over one's mind and over one's body the individual is sovereign.
John Stuart Mill, On Liberty
(1806 - 1873)
My UPDATED UK supplies thread
My first trip report-Amsterdam wanderings
Stonesun's sclerotia infodump
Proudly discovered Highly Sensitive Person ~2009
To all you good gents, I say :hatsoff:

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OfflineVespertineDweller
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Re: First Time Grow Log And Queries (pics) [Re: Ego Questio]
    #11735650 - 12/29/09 02:50 AM (15 years, 22 days ago)

Tossed a couple in a ghetto fruiting chamber i made and that's all well and good... but still these contam jars are driving me nuts. The one that had trich is bursting with shroomies, and the other two the sour rot seems to be being colonized, im gonna birth those in a seperate chamber. thinking about birthing the trich one in a seperate room or something. Input for a noobie anyone?




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"By engaging mycelium we can change the world" -Paul Stamets

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OfflineEgo Questio
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Re: First Time Grow Log And Queries (pics) [Re: VespertineDweller]
    #11735694 - 12/29/09 03:05 AM (15 years, 22 days ago)

The fact they've fruited before the sub is 100% shows the myc has been batteling contams.
I know it's a bitch chucking something when you can see the fruits right there,but if you open that jar,you're unleashing a cloud of trich spores that will contamine your room,get in your hair,on your clothes...basically,you'll spread them all over your house.
Is not worth the future hassle to get at,what,20 grams of fruits?
My opinion...

Did you try PMing any of the help groups after for their advice?


--------------------
Over one's mind and over one's body the individual is sovereign.
John Stuart Mill, On Liberty
(1806 - 1873)
My UPDATED UK supplies thread
My first trip report-Amsterdam wanderings
Stonesun's sclerotia infodump
Proudly discovered Highly Sensitive Person ~2009
To all you good gents, I say :hatsoff:

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OfflineSillyBilly
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Re: First Time Grow Log And Queries (pics) [Re: VespertineDweller]
    #11735702 - 12/29/09 03:07 AM (15 years, 22 days ago)

don't open the trich contamed jar in your house please!
take it outside if you really want to open it up and make sure you wash off those mushrooms if you plan on eating them.
and well at that....
you really don't want to mess with any contams. the bacteria will get you sick of you eat it and the trich is not very good for your health. it has toxins that get released that i know will hurt mushroom colonization and formation but what it would do to people i don't know.


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    You're a better man than I am, Gunga Din

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