Home | Community | Message Board

MushroomCube.com
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Cultivation Supplies   PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Liquid Cultures   Bridgetown Botanicals Bridgetown Botanicals   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineloughbrew
1,1,2,3,5,8,13,21,34,55,89
 User Gallery

Registered: 07/26/09
Posts: 129
Last seen: 2 years, 3 months
My easy to obtain substitute for petri dishes/culture plates.
    #11726756 - 12/27/09 02:52 PM (14 years, 1 month ago)

I have been here for a little bit and even though I have not posted much at all I have been reading as much if not more than most others who come to this site(thanks mania). Hopefully this can help a few of you out, if only in a pinch.
 
  Petri dishes and culture plates are necessary for a few procedures in the science of Mycology, but are often difficult to come by in certain cities (such as the one in which I live). Ordering online is all fine and good, but then you have to wait for it to ship. Not to mention the price of good glass petris and the fact that the plasticware cannot survive the autoclave. One night, under the influence of some natural inspiration, I decided to take a trip to the Walled Market. Not for anything in particular, I just like to be around women and bright lights when I am in that state of mind. As I was making my rounds I picked up a glance of something just after auto. A transparent, multi well, polypropylene box that had a lid which overlapped all sides. A small box for fishing lures. I just stood there for a second and looked at it thinking to myself "No way, it can't be that convenient." But it was. I bought the thing, tested it out, and it worked. This is not as good as glassware or laboratory grade equipment, but they are everywhere. They are also pretty inexpensive. I have been using them ever since.





You should be able to find these in every sporting goods store as well as Wally World, Tarjet and some grocery stores.


--------------------
The meaning of life is a limitation of life.

Nothing that I culture, consume, or store is illegal. Any advice or comments made about such things are simply based on information that others have posted. If I ever say anything that could be misconstrued as evidence that I do such things is a misunderstanding on your part. I am here for the research.


Extras: Filter Print Post Top
Invisiblemyceleus_rex
seeker
Male User Gallery


Registered: 07/01/09
Posts: 1,581
Loc: alized
Re: My easy to obtain substitute for petri dishes/culture plates. [Re: loughbrew]
    #11726767 - 12/27/09 02:55 PM (14 years, 1 month ago)

That fits in a PC?


--------------------
Cervantes' sclerotia thread    Citric's self-healing lid tek  Agar's Grain LC  Breaking Up Spawn Jars

World's Ugliest Cat?

If ignorance is bliss, why aren't there more happy people?


Extras: Filter Print Post Top
Invisiblemister
Nature nut
 User Gallery


Registered: 10/04/08
Posts: 2,926
Re: My easy to obtain substitute for petri dishes/culture plates. [Re: myceleus_rex]
    #11726773 - 12/27/09 02:56 PM (14 years, 1 month ago)

Somehow I don't think it will survive sterilization.


--------------------




:mushroomgrow::mushroomgrow::mushroomgrow::mushroomgrow:AMU Q & A thread:mushroomgrow::mushroomgrow::mushroomgrow::mushroomgrow:


Extras: Filter Print Post Top
Offlineloughbrew
1,1,2,3,5,8,13,21,34,55,89
 User Gallery

Registered: 07/26/09
Posts: 129
Last seen: 2 years, 3 months
Re: My easy to obtain substitute for petri dishes/culture plates. [Re: myceleus_rex]
    #11726783 - 12/27/09 02:58 PM (14 years, 1 month ago)

Its not big, only about 3.5in by 6.25in. I can fit about 10 in my PC at a time. They are polypropylene, and I have PC'd them multiple times.


--------------------
The meaning of life is a limitation of life.

Nothing that I culture, consume, or store is illegal. Any advice or comments made about such things are simply based on information that others have posted. If I ever say anything that could be misconstrued as evidence that I do such things is a misunderstanding on your part. I am here for the research.


Extras: Filter Print Post Top
OfflineRogerRabbitM
Bans for Pleasure
Male User Gallery


Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 22 days
Trusted Cultivator
OG Cultivator
Re: My easy to obtain substitute for petri dishes/culture plates. [Re: myceleus_rex]
    #11726789 - 12/27/09 02:59 PM (14 years, 1 month ago)

But, to open one dish you have to open all five, exposing them to contaminants.  In addition, if one section gets contamination, the spores easily spread to the others.

Plastic petri dishes are sterile when you take them out of the package.  Glass petri dishes get awfully bulky and heavy when you have a few hundred working at once.
RR


--------------------
Download Let's Grow Mushrooms



semper in excretia sumus solim profundum variat

"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison


Extras: Filter Print Post Top
Invisiblemyceleus_rex
seeker
Male User Gallery


Registered: 07/01/09
Posts: 1,581
Loc: alized
Re: My easy to obtain substitute for petri dishes/culture plates. [Re: loughbrew]
    #11726819 - 12/27/09 03:04 PM (14 years, 1 month ago)

Syringes are polypropylene.. should PC fine. I was just thinking today about those PP food containers by Glad and others, plus they come in quarter-pint individual units.


--------------------
Cervantes' sclerotia thread    Citric's self-healing lid tek  Agar's Grain LC  Breaking Up Spawn Jars

World's Ugliest Cat?

If ignorance is bliss, why aren't there more happy people?


Extras: Filter Print Post Top
Offlineloughbrew
1,1,2,3,5,8,13,21,34,55,89
 User Gallery

Registered: 07/26/09
Posts: 129
Last seen: 2 years, 3 months
Re: My easy to obtain substitute for petri dishes/culture plates. [Re: RogerRabbit]
    #11726842 - 12/27/09 03:07 PM (14 years, 1 month ago)

I agree that there is nothing better than labware, this use was surre not in the minds of the company that makes these. While the risk of contamination is increased I do make it a point to use all the wells at once and follow as sterile a procedure as possible. I have a low rate of contamination so I do not mind the risk. I use petris when I can, when I can't I use these.


--------------------
The meaning of life is a limitation of life.

Nothing that I culture, consume, or store is illegal. Any advice or comments made about such things are simply based on information that others have posted. If I ever say anything that could be misconstrued as evidence that I do such things is a misunderstanding on your part. I am here for the research.


Extras: Filter Print Post Top
OfflineBugzy
Magician
Male User Gallery


Registered: 03/18/09
Posts: 436
Last seen: 10 years, 5 months
Re: My easy to obtain substitute for petri dishes/culture plates. [Re: loughbrew]
    #11727480 - 12/27/09 05:22 PM (14 years, 1 month ago)

Why not try one of those over-sized pill organizers, the ones that are divided into days. Then you could use one well at a time.
:2cents:


--------------------
American by Nature....  unamerican by Law :mafioso:

"Out of all the things I've lost, I miss my mind the most."


Extras: Filter Print Post Top
Invisiblebiologys
Mycologist in Trainning
Male

Folding@home Statistics
Registered: 12/21/09
Posts: 4,622
Re: My easy to obtain substitute for petri dishes/culture plates. [Re: Bugzy]
    #11728340 - 12/27/09 08:22 PM (14 years, 1 month ago)

being a big time hunter / fisher there are these exact same trays that has it so each compartment opens seperately just like you stated like the pill containers, they sell them here at gander mountain as 'black powder trays' i guess for the diffrent primers / patches / balls but me being a newbie to this thanks for the great idea


Extras: Filter Print Post Top
InvisibleDoc_T
Random Dude
Male User Gallery


Folding@home Statistics
Registered: 03/06/09
Posts: 42,395
Loc: Colorado Flag
Re: My easy to obtain substitute for petri dishes/culture plates. [Re: myceleus_rex]
    #11728359 - 12/27/09 08:26 PM (14 years, 1 month ago)

Quote:

RogerRabbit said:
But, to open one dish you have to open all five, exposing them to contaminants.  In addition, if one section gets contamination, the spores easily spread to the others.




So do, some don't. I have a bunch (like 40+) of these boxes for one of my other hobbies,and there's different lid styles and different kinds of internal dividers.
Anyone interested, go hit up your local fishing supply place or outdoor shop, they will have an assortment.



Quote:

myceleus_rex said:
Syringes are polypropylene.. should PC fine. I was just thinking today about those PP food containers by Glad and others, plus they come in quarter-pint individual
units.




I've been exploring the PP5 containers, and I'm impressed so far. Haven't fruited one yet, but I have several in progress. (Plus others with stones.)
IF these boxes are PP5 (probably depends on brand and model) they should be atoclaveable.


--------------------
You make it all possible. Doesn't it feel good?


Extras: Filter Print Post Top
Invisiblemyceleus_rex
seeker
Male User Gallery


Registered: 07/01/09
Posts: 1,581
Loc: alized
Re: My easy to obtain substitute for petri dishes/culture plates. [Re: Doc_T]
    #11728401 - 12/27/09 08:42 PM (14 years, 1 month ago)

"IF these boxes are PP5 (probably depends on brand and model) they should be autoclaveable."

That's what he said about his, YMMV.

The food containers sound better as far as cross-contamination goes.
If you have good cultures going, including ATL stones, that's a good sign. I can't see why they wouldn't work.


--------------------
Cervantes' sclerotia thread    Citric's self-healing lid tek  Agar's Grain LC  Breaking Up Spawn Jars

World's Ugliest Cat?

If ignorance is bliss, why aren't there more happy people?


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Cultivation Supplies   PhytoExtractum Buy Bali Kratom Powder   Mushroom-Hut Liquid Cultures   Bridgetown Botanicals Bridgetown Botanicals   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* Re: i this a picture of psilocybe mexicana fruiting in a petri dish? mycofile 4,752 10 02/05/00 11:45 AM
by Mycelium5150
* Petri dish substitute? YouInfoIt 4,455 3 11/13/02 01:50 AM
by JovialLeprechaun
* sterilizing petri dishes llamaboy 10,762 11 01/17/04 02:45 PM
by ragadinks
* useing petri dishes ciscokid670 801 5 10/08/03 12:14 AM
by Cow Shit Collector
* how do you clone without a petri dish phishyfriend 2,122 5 06/15/02 11:39 AM
by Mushroom_Madness
* any downside to using giant petri dishes? e7c 641 1 04/13/04 10:30 AM
by Anno
* Pour agar into petri dishes before pc angelko 6,791 5 05/18/03 05:05 PM
by Prisoner#1
* Re: Petri Dishes & Agar Q's Placebo 3,982 5 12/07/99 10:00 AM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
6,073 topic views. 30 members, 175 guests and 48 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.032 seconds spending 0.009 seconds on 14 queries.