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Offlinefz6
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Am I paranoid?- Grain Lc ?
    #11726118 - 12/27/09 12:49 PM (15 years, 24 days ago)

So afoaf recently made a few pint jars that were half way full with wbs.  Once colonized he added 120 mil of sterilized water to them via a syringe.  He then sucked that out with 8 syringes and shot up some jars with them.  He has used lcs before and noticed that they usually show in 2-3 days.  His grain lcs however have not shown on the 3rd day with temps in from 77-81.  Jars were wbs btw.  The syringes had an opaque color but there was no large chunks of myclium in the water.  Has he done something wrong or just being inpatient?  Are there supposed to be chunks of myc in the water like an actual lc or are pieces so small that you cant really make out the myc?

How long does it take you guys to see growth with a grain lc?

Edited by fz6 (12/27/09 12:57 PM)

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Offlineusudor
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Re: Am I paranoid?- Grain Lc ? [Re: fz6]
    #11726487 - 12/27/09 01:55 PM (15 years, 24 days ago)

you did it right.
if after a week they have no growth then you know your glc was bad.


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Edited by usudor (12/27/09 01:56 PM)

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Offlinefz6
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Re: Am I paranoid?- Grain Lc ? [Re: usudor]
    #11727430 - 12/27/09 05:14 PM (15 years, 24 days ago)

What could go wrong with a glc though?  I noticed that the myc in the glc syringe is tiny compared to what I normally get from and LC.  I dont mean volume of myc, I mean size of the strands of myc.  Does it just take longer for glcs to take off because they start with such tiny pieces of myc?

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InvisibleShea25
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Re: Am I paranoid?- Grain Lc ? [Re: fz6]
    #11727443 - 12/27/09 05:16 PM (15 years, 24 days ago)

I have used LC's that have taken 4-5 days to start give it time

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Offlinefz6
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Re: Am I paranoid?- Grain Lc ? [Re: Shea25]
    #11727463 - 12/27/09 05:19 PM (15 years, 24 days ago)

Alright.  I hate waiting!!  Especially when Im trying something new and I don't know what to expect.  Today was  only the 3rd day so if by tuesday or wednesday there isn't even a speck showing then Ill know that somethings up.  Ill keep the board posted!

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OfflineBaKeRx561x420
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Re: Am I paranoid?- Grain Lc ? [Re: fz6]
    #11727495 - 12/27/09 05:24 PM (15 years, 24 days ago)

What gauge needle did you use?


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Invisibledancefloordale
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Re: Am I paranoid?- Grain Lc ? [Re: BaKeRx561x420]
    #11727546 - 12/27/09 05:36 PM (15 years, 24 days ago)

Completely normal in my experience. My grain LC's are always slower to show growth than sugar based ones. :thumbup:

If you shook it up well enough after adding the water the needle size shouldn't matter too much. I've had success with 20g needles using this method, but wouldn't recommend them.


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Everything posted by the user dancefloordale, aside from what is written in this here clause is completely and utterly fictitious, despite any information read (or seen), above (or below) that might lead you to believe otherwise.

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Edited by dancefloordale (12/27/09 05:38 PM)

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Offlinefz6
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Re: Am I paranoid?- Grain Lc ? [Re: dancefloordale]
    #11728644 - 12/27/09 09:29 PM (15 years, 23 days ago)

I think it was a 16 guage.  Just a standard 12 cc syringe from a farm supply store.  They were allowed to sit with the water in the jars for about 30 mintutues and then swirled for about 5 minutes straight.  The water was then sucked up using my syinges.  So whats normal for you dancefloordale, mabye 5 or 6 days?

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OfflineMontock
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Re: Am I paranoid?- Grain Lc ? [Re: fz6]
    #11728816 - 12/27/09 09:55 PM (15 years, 23 days ago)

my glc took for ever to show growth but when it did it exploded, yours sounds normal, i just added a bunch of water shook the shit out of it till i saw 905 or better of the myc stripped off and sucked it up and shot up a few jars, i also shot all the rest of the water back into the sterile water jar, what im going to do next time is add water shake the shit out of it, and shoot it all back into the sterile water jar and wait a day or to than suck the myc up and than shoot something up, after wards the grain recovered anyone know if its safe to make more glc out of it, looks ugly but healthy


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Invisibledancefloordale
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Re: Am I paranoid?- Grain Lc ? [Re: fz6]
    #11728852 - 12/27/09 10:02 PM (15 years, 23 days ago)

Quote:

Montock said:
my glc took for ever to show growth but when it did it exploded, yours sounds normal, i just added a bunch of water shook the shit out of it till i saw 905 or better of the myc stripped off and sucked it up and shot up a few jars, i also shot all the rest of the water back into the sterile water jar, what im going to do next time is add water shake the shit out of it, and shoot it all back into the sterile water jar and wait a day or to than suck the myc up and than shoot something up, after wards the grain recovered anyone know if its safe to make more glc out of it, looks ugly but healthy




Should be ok to use it again. I have spawned recovered grain jars before without problems.

Quote:

fz6 said:
I think it was a 16 guage.  Just a standard 12 cc syringe from a farm supply store.  They were allowed to sit with the water in the jars for about 30 mintutues and then swirled for about 5 minutes straight.  The water was then sucked up using my syinges.  So whats normal for you dancefloordale, mabye 5 or 6 days?




Yeah 5 or 6 days and you should see some growth. :thumbup:


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Everything posted by the user dancefloordale, aside from what is written in this here clause is completely and utterly fictitious, despite any information read (or seen), above (or below) that might lead you to believe otherwise.

Hydra Tek - A detailed guide, for newest to the most skilled cultivators.

HCA

Bulk growing made easy-discussion
Bulk Growing Made Easy

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Offlinefz6
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Re: Am I paranoid?- Grain Lc ? [Re: fz6]
    #11728984 - 12/27/09 10:32 PM (15 years, 23 days ago)

Cool.  What If I was to suck back up the water using my large 60cc syringes and then shot that into a Honey Lc and let it colonize that for say one week.  By this time the lc should look CRAAAZY thick and would possibly have the regular lcs reaction time.  Does this sound reasonable?

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Offlinefz6
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Re: Am I paranoid?- Grain Lc ? [Re: fz6]
    #11729003 - 12/27/09 10:36 PM (15 years, 23 days ago)

I thought Id also mention that 1cc was used on pint jars.  That seemed like a decent amount to use.  Sound about right to you?

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Re: Am I paranoid?- Grain Lc ? [Re: fz6]
    #11729018 - 12/27/09 10:40 PM (15 years, 23 days ago)

as for the glc to honey, it will grow till its out of nutes or air, or something goes wrong, it should look normal just get there a bit quicker, as for the amount of lc/ms solution you use, the more the better in my book as long as your not fucking your moisture content up


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Offlinefz6
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Re: Am I paranoid?- Grain Lc ? [Re: Montock]
    #11729052 - 12/27/09 10:50 PM (15 years, 23 days ago)

I realized later that double the solution could have been used and there still would have been tons of solution left over.  You live you learn right?  Its hard to tell whats what when looking at the glc syringe.  The whole syringe has that opaque myc color to it but the strands are so small that Im not sure a lot/most of the color is from dirt and crap from the seed and not myc.  In regular Lcs you can visibly see the myc floating around, I like that.

Can you imagin shooting a 60cc glc syringe into a 200ml lc though?  2 weeks later the thing would by so thick you couldn't see through it.

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Invisiblemyceleus_rex
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Re: Am I paranoid?- Grain Lc ? [Re: fz6]
    #11729059 - 12/27/09 10:54 PM (15 years, 23 days ago)

Funny, I've only done a couple g LCs but they seemed to colonize spawn faster than sugar LC-- one started within 48 hrs. Other threads I read confirmed this. My understanding is that starting the culture on grain acclimates the myc to colonizing grain substrate, so it "hits the ground running".


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Offlinefz6
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Re: Am I paranoid?- Grain Lc ? [Re: myceleus_rex] * 1
    #11737009 - 12/29/09 12:12 PM (15 years, 22 days ago)

I just thought that Id let everyone know that on day 4 the first specks were found. On day 5, today the seemed to start to really take off.  By tomorrow I imagine they will be at full throttle.  So 5-6 days is about right then at 77-80 degrees.
Peace!

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