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OfflineBugzy
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What is a good sterile technique for spawning?
    #11721288 - 12/26/09 01:04 PM (15 years, 25 days ago)

I have got most of my sterile techniques down, but still skeptical of spawning to bulk. Moving the substrate from pastuerization to F/C and the spawn from the jars too the substrate without involving that much "open-air" time, is my main concern.

~I have been keeping the lid on my coir pot till I am ready too spawn
~Spray the room down good and get everything together
~Place an open trash bag in my mono, pour coir/spawn into garbage bag and mix well and level w/gloves.
~close up bag and poke holes for GE, place a lid on and wait a couple weeks before I check on it

My success has been 50/50 so far, not sure where I am goin wrong yet. Start by eliminating my spawning technique and go from there. I just have a few questions...

What are some good ways too spawn to bulk?

Whats the risk of:
  -grain contaminating after colonization?
  -substrates in general contaminating after pastuerization?
  -contamination after spawning?

One last thing, whats the deal with trich-enriched coir? Good/bad; does it effect anything?

Thanks for any help.


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InvisibleCitizen13
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Registered: 11/01/09
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Re: What is a good sterile technique for spawning? [Re: Bugzy]
    #11721299 - 12/26/09 01:06 PM (15 years, 25 days ago)

trich enriched coir? yeah stay away from that.

i just pastuerize my coir, let it cool in a paint straining bag overnight in the sink. then in the morning i break up my spawn in the bag/jar and pour it into the bottom of my tub, pour in the coir on top of that, mix with my hands, place the tub in a garbage bag and forget about it for a week.


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Offlinetrashcan
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Re: What is a good sterile technique for spawning? [Re: Citizen13]
    #11721352 - 12/26/09 01:20 PM (15 years, 25 days ago)

ok, so my technique is a little different than yours, but im pretty sure it could be tweaked for what you want. i dont do grain yet im still working with brf cakes, but the same can be done with both.

i take a gallon size freezer bag and put my jar inside and birth in the bag, then crumble the cake in that jar. i then take my pasturized poo (put in a pillowcase and pasturized at 150 for 90 min and allowed to cool in a strainer in the sink) and add 3 jars per 1 jar of colonized brf, and mix that in the bag. after it is mixed well, pebble to small gravel sized chunks at largest, i then put it into a jumbo alumin foil bread pan, cover with labeled foil, and poke a couple holes into it, add maybe 3 or 4 bits of water, i always just add a little extra (which was nuked for 5 min) and set it away.


heres a link to the actual tek.  bulk for noobs    sorry that was a really bad run on sentance.


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InvisibleCitizen13
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Re: What is a good sterile technique for spawning? [Re: trashcan]
    #11721372 - 12/26/09 01:27 PM (15 years, 25 days ago)

with brf cakes i would throw them in a food processor for best results.

too much sterility during the spawning phase is silly imo. just get as many noc points as you can and leave it alone for a week. the high co2 and the high spawn/substrate ratio will do the rest.


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Offlinedutc2006
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Re: What is a good sterile technique for spawning? [Re: trashcan]
    #11721374 - 12/26/09 01:27 PM (15 years, 25 days ago)

Some people go as far from sterile procedures as to just pour boiling water into their coir or sub, mix and then let it set, then drain.  I guess it is safe to say that if you mix your colonized grain in well with the sub and you use enough, that the myc is going to colonize the sub faster than any competitors will have a chance to.  Your fully colonized grains are pretty contam resistant alone because the myc already has a foothold.  I just use a clean wooden spoon in open air when mixing my spawn and sub.  I just tried this for the first time with coir, the boiling water thing, and it is working fine.  Already 100% colonized.

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Offlinetrashcan
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Re: What is a good sterile technique for spawning? [Re: dutc2006]
    #11721387 - 12/26/09 01:31 PM (15 years, 25 days ago)

the reason i put the water in the micro for a bit before i add just that bit more is because my area has terribly hard bad water, and i just dont like wasting time and effort, better safe than sorry ya know?


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i will do ANYTHING for some pan cyans, plese...

p.m. me with any questions, ill help with what i can. especially newbs.



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InvisibleM11
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Re: What is a good sterile technique for spawning? [Re: trashcan]
    #11721612 - 12/26/09 02:27 PM (15 years, 25 days ago)

I pasteurize in a big pot.  I let the temperature reach 160 F.  I make sure the temperature is evenly distributed throughout the substrate then turn off the stove.  DO NOT open the pot until you are ready to spawn. 

I get all my jars and my tub ready (with a black trash back).  I actually wipe down the trash bag with some iso and let it dry.  I also wipe down the entire interior of the tub with iso.  Make sure the iso has evaporated before spawning.  I open my jars and dig out the spawn with a sterilized spoon (boiled for 30 minutes).  I bleach my entire work area with a 1:4 solution of bleach to water.  I also spray the shit out of the air with Oust and cover all the vents with alcohol soaked towels. 

Wear a face mask and gloves.  Layer your spawn and sub.  I know it sounds crazy to be that sterile, but no contams so far for me.

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InvisibleCitizen13
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Re: What is a good sterile technique for spawning? [Re: M11]
    #11721629 - 12/26/09 02:30 PM (15 years, 25 days ago)

i do none of that and i don't even wash my hands.

i get no contams either.

:shrug:

figure out what works for you and stick with it :laugh:


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Invisibleanonjon
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Re: What is a good sterile technique for spawning? [Re: Citizen13]
    #11721643 - 12/26/09 02:34 PM (15 years, 25 days ago)

Quote:

Citizen13 said:
i do none of that and i don't even wash my hands.

i get no contams either.

:shrug:

figure out what works for you and stick with it :laugh:




same , if you're getting 50/50 its from using trich enriched coir for sure. It's a no brainer.


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The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

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InvisibleCitizen13
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Re: What is a good sterile technique for spawning? [Re: anonjon]
    #11721651 - 12/26/09 02:36 PM (15 years, 25 days ago)

Quote:

anonjon said:
Quote:

Citizen13 said:
i do none of that and i don't even wash my hands.

i get no contams either.

:shrug:

figure out what works for you and stick with it :laugh:




same , if you're getting 50/50 its from using trich enriched coir for sure. It's a no brainer.




a 1:4 ratio actually fully colonizes my tubs in 2-3 days. i give it an extra week just to be sure.


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OfflineNoBrainNoPain
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Re: What is a good sterile technique for spawning? [Re: Citizen13]
    #11721672 - 12/26/09 02:41 PM (15 years, 25 days ago)

I do mostly like Trashcan, but with a little variant…

I follow RRs "bain-marie" technique to pasteurize my coir/verm/coffee mix at correct moisture, but instead of glass jars, I put the mix in an oven bag, in a pot of hot water.

Then I clean my spawn jars and empty them directly inside the bag. Mix, then fill in the tub.

No more open-air time :grin:


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Invisibleanonjon
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Re: What is a good sterile technique for spawning? [Re: NoBrainNoPain]
    #11721702 - 12/26/09 02:48 PM (15 years, 25 days ago)

Is there a more sterile way to spawn?

Step 1: don't add trich to your substrate.


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The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

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OfflineBugzy
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Re: What is a good sterile technique for spawning? [Re: Citizen13]
    #11721899 - 12/26/09 03:46 PM (15 years, 25 days ago)

Quote:

Citizen13 said:
Quote:

anonjon said:
Quote:

Citizen13 said:
i do none of that and i don't even wash my hands.

i get no contams either.

:shrug:

figure out what works for you and stick with it :laugh:




same , if you're getting 50/50 its from using trich enriched coir for sure. It's a no brainer.




a 1:4 ratio actually fully colonizes my tubs in 2-3 days. i give it an extra week just to be sure.




How deep is your sub? I have yet too see a tub colonize in less than 10days. My sub depth is around 4" give or take. Maybe not enough moisture?

I am not sure if the coir I was using had trich in it or not, got it from Petco. What brand of coir isnt enriched with trich?

Does water type make a difference? The tap water around here is terrible, looks like watered-down jizz. I have heard of people using natural pond/river water and distilled water for hydrating subs instead of tap.


--------------------
American by Nature....  unamerican by Law :mafioso:

"Out of all the things I've lost, I miss my mind the most."

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Invisiblecyantific
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Re: What is a good sterile technique for spawning? [Re: Bugzy]
    #11721936 - 12/26/09 03:55 PM (15 years, 25 days ago)

simply turning off the ac/heat/fan in your home is a good step a lot of newer ppl never think of ... most ducts are in the ceiling raining down contaminates into open bins/trays ... also dont frost layer ... always cover your broken up spawn with pastuerized sub ... the myc is in its weakest most vulnreble stage then ...

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OfflineBugzy
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Re: What is a good sterile technique for spawning? [Re: cyantific]
    #11721980 - 12/26/09 04:03 PM (15 years, 25 days ago)

Quote:

cyantific said:
simply turning off the ac/heat/fan in your home is a good step a lot of newer ppl never think of ... most ducts are in the ceiling raining down contaminates into open bins/trays ... also dont frost layer ... always cover your broken up spawn with pastuerized sub ... the myc is in its weakest most vulnreble stage then ...




Not sure what you mean by frost layer?

I do wait till the air settles after turning shit off as well as cover spawned sub with plain sub. Is there any need too place wax paper or anything ontop of that, or is that just for trays?


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American by Nature....  unamerican by Law :mafioso:

"Out of all the things I've lost, I miss my mind the most."

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Invisiblemyceleus_rex
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Re: What is a good sterile technique for spawning? [Re: Bugzy]
    #11722094 - 12/26/09 04:32 PM (15 years, 25 days ago)

"frost layer" just means coating the top of your sub with a dusting of spawn.


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OfflineBugzy
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Re: What is a good sterile technique for spawning? [Re: myceleus_rex]
    #11722193 - 12/26/09 04:57 PM (15 years, 25 days ago)

Ok, that makes sense. When you said "dont frost layer..." I thought there was somthing that I shouldnt be doing.


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American by Nature....  unamerican by Law :mafioso:

"Out of all the things I've lost, I miss my mind the most."

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Offlinedman080
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Re: What is a good sterile technique for spawning? [Re: Bugzy]
    #11722497 - 12/26/09 06:08 PM (15 years, 25 days ago)

what is this trich enriched coir???

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InvisibleCitizen13
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Re: What is a good sterile technique for spawning? [Re: dman080]
    #11722511 - 12/26/09 06:10 PM (15 years, 25 days ago)

Quote:

dman080 said:
what is this trich enriched coir???




it will say right on the packaging. it can be beneficial for some plant growing purposes. its the reason alot of people mistakenly think coir is trich-prone.

which it isn't.

at all


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OfflineBugzy
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Re: What is a good sterile technique for spawning? [Re: Citizen13]
    #11722559 - 12/26/09 06:20 PM (15 years, 25 days ago)

Is coco-tek good coir? I think thats the name anyways...


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American by Nature....  unamerican by Law :mafioso:

"Out of all the things I've lost, I miss my mind the most."

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InvisibleCitizen13
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Re: What is a good sterile technique for spawning? [Re: Bugzy]
    #11722568 - 12/26/09 06:22 PM (15 years, 25 days ago)

Quote:

Bugzy said:
Is coco-tek good coir? I think thats the name anyways...




i love the stuff


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OfflineRogerRabbitM
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Re: What is a good sterile technique for spawning? [Re: Citizen13]
    #11723153 - 12/26/09 08:46 PM (15 years, 24 days ago)

If you properly pasteurize your coir at 140F to 160F for one hour and you still get green mold before first flush, then there is no doubt about it:  Your spawn(noun) is contaminated before you ever spawn(verb) to bulk.
RR


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OfflineBugzy
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Re: What is a good sterile technique for spawning? [Re: RogerRabbit]
    #11734528 - 12/28/09 10:11 PM (15 years, 22 days ago)

2 things too add:

Coir doesnt matter if it has trich already aslong as you pastuerize correctly, correct?

Does water type make a difference? The tap water around here is terrible, looks like watered-down jizz. I have heard of people using natural pond/river water and distilled water for hydrating subs instead of tap. Have been thinking about just using distilled H2o from begining to end.

One other thing, So nothing can breed on pastuerized subs because all the resources for the mold spores to maintain life are cooked out leaveing the viable resources for shoomr too grow correct? And they cant over come mycellium once its colonized,even when you break up your jars and see the grain through the myc, mold cant find its way in?

sounds like things are mold proof after a few simple steps. Which leads me too the confusion of how do molds get there after everything is colonized? or what type of molds. Assuming your spawn was good.?

Not sure if i said what I was trying too, but I ll see soon I guess

Thanks for your time


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Invisibleanonjon
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Re: What is a good sterile technique for spawning? [Re: Bugzy]
    #11734551 - 12/28/09 10:17 PM (15 years, 22 days ago)

I'm sure some molds manage to hatch in the sub, but they are so outnumbered by the myc, which is already established and growing vigorously. So it overtakes the whole sub and becomes pretty resistant to contamn.

But then you get a couple flushes and the sub is tired and dirty and trich manages to set in.

Game over. Toss it.

I think using the trich enriched coir is still a mistake. If you do use it, I would keep temps closer to 180 the whole time.


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The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

Edited by anonjon (12/28/09 10:18 PM)

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OfflineBugzy
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Re: What is a good sterile technique for spawning? [Re: anonjon]
    #11735164 - 12/29/09 12:16 AM (15 years, 22 days ago)

Quote:

anonjon said:
I'm sure some molds manage to hatch in the sub, but they are so outnumbered by the myc, which is already established and growing vigorously. So it overtakes the whole sub and becomes pretty resistant to contamn.

But then you get a couple flushes and the sub is tired and dirty and trich manages to set in.

Game over. Toss it.

I think using the trich enriched coir is still a mistake. If you do use it, I would keep temps closer to 180 the whole time.




I m with you on the anti trch coir all the way.

So you dont necassrily get rid of mold, you hold it off for the mushrooms too take over before the mold spores catch up right?


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American by Nature....  unamerican by Law :mafioso:

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Invisibleblackdust
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Re: What is a good sterile technique for spawning? [Re: RogerRabbit]
    #11735294 - 12/29/09 01:00 AM (15 years, 22 days ago)

Quote:

RogerRabbit said:
If you properly pasteurize your coir at 140F to 160F for one hour and you still get green mold before first flush, then there is no doubt about it:  Your spawn(noun) is contaminated before you ever spawn(verb) to bulk.
RR





What happens if the temp goes over 160F? Sometimes i get up to 190F. Is that bad?

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OfflineBugzy
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Re: What is a good sterile technique for spawning? [Re: blackdust]
    #11735369 - 12/29/09 01:29 AM (15 years, 22 days ago)

your fine, 140-160 is optimal, 170 is pointless line, but 190 wont hurt, just try too keep a better eye on it next time


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American by Nature....  unamerican by Law :mafioso:

"Out of all the things I've lost, I miss my mind the most."

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Invisibleblackdust
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Re: What is a good sterile technique for spawning? [Re: Bugzy]
    #11735393 - 12/29/09 01:36 AM (15 years, 22 days ago)

Quote:

Bugzy said:
your fine, 140-160 is optimal, 170 is pointless line, but 190 wont hurt, just try too keep a better eye on it next time




Its my stove. I always turn it off once it hits 140F but then it keeps going and going and going. Next time I will turn off stove sooner.

Good Info!

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OfflineBugzy
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Re: What is a good sterile technique for spawning? [Re: blackdust]
    #11735401 - 12/29/09 01:39 AM (15 years, 22 days ago)

Wait.. whats goin on? you turn the stove of at 140? and then it goes up too 190?

how are pastuerizing; ovenbag, pot?


--------------------
American by Nature....  unamerican by Law :mafioso:

"Out of all the things I've lost, I miss my mind the most."

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