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anakin3197
JEDI MASTER


Registered: 08/21/08
Posts: 1,425
Last seen: 9 months, 3 days
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what do you all think could be wrong?
#11718400 - 12/25/09 08:45 PM (15 years, 25 days ago) |
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so I started out messing up by using wbs that was soaked for 5 days, and on a whim i decided not to simmer it, but pcd as i normally do, but i overcooked it ad it actually looked pretty dry, but i decided to use it anyway. i knocked up the jars with 2 differet strains, so i dont think it is the lcs that i used since they are both like that.



also i am wondering, i need to make jus 2 plates, and i have this http://sporeworks.com/store/catalog/Potato-Dextrose-Agar-p-16215.html
and i was jus wondering how much i should use for each plate and how much water?
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noobieshroomie
Back again




Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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Re: what do you all think could be wrong? [Re: anakin3197]
#11718427 - 12/25/09 08:53 PM (15 years, 25 days ago) |
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looks like trich to me if youre PCing long enough its getting contaminated when you are knocking them up
as for the agar, do you have a scale?
-noobie-
-------------------- AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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anakin3197
JEDI MASTER


Registered: 08/21/08
Posts: 1,425
Last seen: 9 months, 3 days
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Re: what do you all think could be wrong? [Re: anakin3197]
#11718428 - 12/25/09 08:55 PM (15 years, 25 days ago) |
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hand held not digital
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noobieshroomie
Back again




Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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Re: what do you all think could be wrong? [Re: anakin3197]
#11718505 - 12/25/09 09:08 PM (15 years, 25 days ago) |
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to find out how much you need you have to break it down i gave you 14g=2tlbs thats added to 500ml water gives you20 dishes
so take 1tlbs=7g and add 250ml water gives you 10 dishes
so take 1.5tsp =3.5g to 125ml water should give you about 5 dishes
-noobie-
-------------------- AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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anakin3197
JEDI MASTER


Registered: 08/21/08
Posts: 1,425
Last seen: 9 months, 3 days
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Re: what do you all think could be wrong? [Re: noobieshroomie]
#11718592 - 12/25/09 09:31 PM (15 years, 25 days ago) |
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ok thank you
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noobieshroomie
Back again




Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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Re: what do you all think could be wrong? [Re: anakin3197]
#11718629 - 12/25/09 09:39 PM (15 years, 25 days ago) |
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Merry Christmas bro
-noobie-
-------------------- AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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piyox420
seeker



Registered: 03/27/09
Posts: 34
Loc: pacific northwest
Last seen: 3 years, 2 months
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Re: what do you all think could be wrong? [Re: noobieshroomie]
#11719076 - 12/25/09 11:21 PM (15 years, 25 days ago) |
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hah...merry christmasss
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anakin3197
JEDI MASTER


Registered: 08/21/08
Posts: 1,425
Last seen: 9 months, 3 days
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Re: what do you all think could be wrong? [Re: piyox420]
#11719105 - 12/25/09 11:29 PM (15 years, 25 days ago) |
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merry christmas to you all as well.
i think that im jus going to go ahead and make 10 dishes. so that i can go ahead and try to check the lcs as well. and im going to throw away all those jars, thats the second time thats shits happened to those jars.
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