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OfflineSmawrpg
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Started first grow w/some qs
    #11717530 - 12/25/09 04:23 PM (15 years, 26 days ago)

I am using a Mycobag from the mad season, its only a 1lb bag.

I inoculated it with 2-3ccs of spore solution between bag and substrate, I only shook the syringe a little bit.

I did not flame sterilize only used 70% rubbing alcohol and a glove box (crappy cardboard one)

Once I sterilized the injection site I kept the alcohol-drenched wipe on top of it until I could clean the syringe and inject the solution.

I am keeping the bag at around 66-68 degrees, Its kind of cold I know but I am short on space and heating oil money. Plus I hear bags are always 4-5 degrees warmer once growth appears, not sure if thats true however.

How long before I can expect to see growth if I inoculated it on 48 hours ago?  Currently there is nothing. I hear up to two weeks is that right?  Also how many bags just flat out do not colonize not considering contamination, just no growth whatsoever, is that somewhat common?

Thanks guys, im just very impatient and nervous as bags and spores arn't cheap and I really want some mushies.

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OfflineSmawrpg
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Re: Started first grow w/some qs [Re: Smawrpg]
    #11717716 - 12/25/09 05:10 PM (15 years, 26 days ago)

bump?

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OfflineParapipe
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Re: Started first grow w/some qs [Re: Smawrpg]
    #11717739 - 12/25/09 05:17 PM (15 years, 26 days ago)

As far as growth goes you may see tiny patches of mycelium in as little as 3 days in my experience however, due to the cooler incubation conditions you have mentioned 66-68 degrees F visible growth may take a few days longer. If you inspect it every day it will be like trying to boil water while watching the pot and feel as though its taking for ever. Your best bet is to set it and forget it (at least for a while keep your self busy) but, it would also be wise to inspect your bag(s) every so often for growth as well as keeping an eye out for contamination. May my wishes for success be with you good luck.


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Sincerely, Your friend Parapipe.

Edited by Parapipe (12/25/09 05:19 PM)

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OfflineSmawrpg
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Re: Started first grow w/some qs [Re: Parapipe]
    #11720302 - 12/26/09 07:00 AM (15 years, 25 days ago)

Okay cool.  Is it ok if I just pick it up very gently to inspect it as I have done that a couple times? I try not to move the contents at all.

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InvisibleCitizen13
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Re: Started first grow w/some qs [Re: Smawrpg]
    #11720328 - 12/26/09 07:19 AM (15 years, 25 days ago)

Quote:

Smawrpg said:
Okay cool.  Is it ok if I just pick it up very gently to inspect it as I have done that a couple times? I try not to move the contents at all.




yup. won't hurt a thing.

remember in the future when sterilizing your needle before injection, that nothing gets more sterile then a red hot piece of metal. I flame the tip and then cool it with an alcohol swab.


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OfflineSmawrpg
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Re: Started first grow w/some qs [Re: Citizen13]
    #11728776 - 12/27/09 09:48 PM (15 years, 23 days ago)

cool thanks I will sterilize better next time.

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Invisiblegus330
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Re: Started first grow w/some qs [Re: Smawrpg]
    #11728862 - 12/27/09 10:05 PM (15 years, 23 days ago)

I used the same bags about 6months ago and used them for spawn in my mono tub great results


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OfflineMontock
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Re: Started first grow w/some qs [Re: gus330]
    #11729005 - 12/27/09 10:37 PM (15 years, 23 days ago)

It's true it does sometime take up to 2 weeks with a ms syringe, be pateint, if i were you i personally would stow it away and forget about it and set a reminder with your cell phone, it makes the time go by alot quicker, you can pick it up and look at it, but i find to have better results when i dont fuck with them, also shaking your syringe very thourghly helps a ton and being anal about being clean is a must.


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OfflineSmawrpg
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Re: Started first grow w/some qs [Re: Montock]
    #11742491 - 12/30/09 12:55 PM (15 years, 21 days ago)

Alright I knows its probably too soon to be worried but its been a week and no signs of mycellium, should I shake the bag?  Also when should I put reinoculation on the table, another week? Thanks.

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InvisibleDoc_T
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Re: Started first grow w/some qs [Re: Smawrpg]
    #11742519 - 12/30/09 12:59 PM (15 years, 21 days ago)

Shake at around 25-33% growth.
Re-inoculation with the same syringe makes no sense to me. But possibly with another one if there's no growth visible in another week.

But 66 is cold. The bag can't warm up until it starts growing, and it can't grow until it warms up.
So put it someplace like a box or a styrofoam cooler. With a hot water bottler, not a heater. And use a thermometer.

Somebody is going to come along and say 66 is fine, that they grow mushrooms at the North Pole, and it never gets above freezing.
You still want warmer temps, no matter what they say.


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Invisiblegus330
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Re: Started first grow w/some qs [Re: Doc_T]
    #11742610 - 12/30/09 01:15 PM (15 years, 21 days ago)

hey Doc_T having problems with my mono tub staying higher than 70degrees any ideas would help its a mini mono 6 qt


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InvisibleDoc_T
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Re: Started first grow w/some qs [Re: gus330]
    #11742645 - 12/30/09 01:21 PM (15 years, 21 days ago)

Warmer room? 70 isn't bad, but 75 would probably be better. What are temps like right where the tub is?


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Invisiblegus330
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Re: Started first grow w/some qs [Re: Doc_T]
    #11742773 - 12/30/09 01:39 PM (15 years, 21 days ago)

68 degrees f


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InvisibleDoc_T
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Re: Started first grow w/some qs [Re: gus330]
    #11742793 - 12/30/09 01:43 PM (15 years, 21 days ago)

Yeah, that's on the cool side. Could you raise it up a bit? Couple of feet = couple of degrees, usually.


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Invisiblegus330
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Re: Started first grow w/some qs [Re: Doc_T]
    #11742809 - 12/30/09 01:46 PM (15 years, 21 days ago)

yea i,ll give it a try thanks for the help if that doe,nt work can you use like a heating pad on low under a blanket or something


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OfflineSmawrpg
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Re: Started first grow w/some qs [Re: gus330]
    #11745579 - 12/30/09 09:51 PM (15 years, 20 days ago)

ill take that in advice and somehow work on keeping it warm.  Doc what is that girl in ur avatar, I see her everywhere but have yet to know thats all about, kinda random and off topic but just wondering.

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