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Offlinefearforce7
addict
Registered: 01/30/00
Posts: 642
Loc: USA
Last seen: 7 years, 11 months
Is this why all my jars got contams?
    #796055 - 08/04/02 03:00 PM (21 years, 7 months ago)

I realized i did not flame the needle when i went to inoculate, i just rubed it down with alchole(sp?) anyway anyone think this is why they all got infected?


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Offlineravingbanana
Every Girl'sSexual Desire

Registered: 07/25/02
Posts: 249
Last seen: 20 years, 9 months
Re: Is this why all my jars got contams? [Re: fearforce7]
    #796058 - 08/04/02 03:02 PM (21 years, 7 months ago)

As long as it's not a very low % of alcohol I don't think that would have been the reason.. I don't think you should've flamed it after the alcohol wipe. Even though that would've been a pretty funny reaction from you.


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If Bin Laden eats worms.. Would he be equal to nutritional value of worm castings?
If you could have sex with a person's voice.. Your voice would be the one I'd pick to rape.
Don't worry.. I have a seven inch penis.

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Offlinefearforce7
addict
Registered: 01/30/00
Posts: 642
Loc: USA
Last seen: 7 years, 11 months
Re: Is this why all my jars got contams? [Re: ravingbanana]
    #796068 - 08/04/02 03:06 PM (21 years, 7 months ago)

it was 70%


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Offlineravingbanana
Every Girl'sSexual Desire

Registered: 07/25/02
Posts: 249
Last seen: 20 years, 9 months
Re: Is this why all my jars got contams? [Re: fearforce7]
    #796077 - 08/04/02 03:12 PM (21 years, 7 months ago)

Were you cautious as you were doing it? Making sure the syringe didn't touch anything before using it at an innoculation point.. and wiping it down at each point?


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If Bin Laden eats worms.. Would he be equal to nutritional value of worm castings?
If you could have sex with a person's voice.. Your voice would be the one I'd pick to rape.
Don't worry.. I have a seven inch penis.

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Offlinefearforce7
addict
Registered: 01/30/00
Posts: 642
Loc: USA
Last seen: 7 years, 11 months
Re: Is this why all my jars got contams? [Re: ravingbanana]
    #796108 - 08/04/02 03:27 PM (21 years, 7 months ago)

Well, i didnt wipe it down each time, to tell you the truth, i feel as though i was kinda sloppy with it. The kitchen was dirty, but i used a lot of lysoel. I think i just need to be cleaner next time, Oh yea, I used the oven tek. anyway

later


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Offlineravingbanana
Every Girl'sSexual Desire

Registered: 07/25/02
Posts: 249
Last seen: 20 years, 9 months
Re: Is this why all my jars got contams? [Re: fearforce7]
    #796120 - 08/04/02 03:34 PM (21 years, 7 months ago)

Priority number one should have been being clean. Once you get used to how clean you should be then you should be able to do it quicker and remember.. All good things take time.


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If Bin Laden eats worms.. Would he be equal to nutritional value of worm castings?
If you could have sex with a person's voice.. Your voice would be the one I'd pick to rape.
Don't worry.. I have a seven inch penis.

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OfflineKilla420
Alien Lover
Male User Gallery
Registered: 06/26/02
Posts: 1,057
Loc: Down South
Last seen: 1 year, 3 months
Re: Is this why all my jars got contams? [Re: ravingbanana]
    #796136 - 08/04/02 03:41 PM (21 years, 7 months ago)

I never flame sterilize my needle and have never gotten contams except for when I used to much water in my birdseed. I just wipe the needles after ever jar with alcohol. I do this in a small ass closet that doesnt have anything in it at all. I cleaned it out with bleach a long time ago so I just spray lysol when im innaculating and it works fine. I would suggest not doing that in your kitchen. To big of a area that the lysol might not be able to cover. Try a bathroom or closet like I do. Good luck next time.

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Offlinepleezr
fuct

Registered: 12/22/01
Posts: 1,708
Loc: NW
Last seen: 19 years, 1 month
Re: Is this why all my jars got contams? [Re: fearforce7]
    #796137 - 08/04/02 03:41 PM (21 years, 7 months ago)

No. I never flame the needle. Just wipe that bitch.


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pleezr

"Tell me about the fucking golf shoes!!"

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Offlinefearforce7
addict
Registered: 01/30/00
Posts: 642
Loc: USA
Last seen: 7 years, 11 months
Re: Is this why all my jars got contams? [Re: pleezr]
    #796220 - 08/04/02 04:17 PM (21 years, 7 months ago)

Well, that was my second set of jars. The first ones i did worked really good, but i also cleaned really good so i gues that is why, i am doing it in the kitchen so i can use the oven rack. Well anway, i got a free syring when i ordered them so i am gonna make up some more jars tonight and just clean better. BTW they are south americans

later


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Offlinetchyted
miestro
Registered: 09/03/01
Posts: 526
Loc: WA near seattle
Last seen: 9 years, 6 months
Re: Is this why all my jars got contams? [Re: fearforce7]
    #796406 - 08/04/02 05:39 PM (21 years, 7 months ago)

it has been my experiance that the more one f%&ks with his proceedure, the more he invites contamnation upon his head!

woa! yea, woe! unto the pittlers in the laboratory, for theirs shal be undone!

who the hell flames or wipes his needles?

if you have any reason to suspect that a needle is contamned, then quit using it. otherwise the syringe is out of it's sterile container only for the short time it is to be used, ideally never leaving the inocculators hand.

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OfflineZEPH
member

Registered: 03/01/02
Posts: 1,609
Loc: Up
Last seen: 8 years, 6 months
Re: Is this why all my jars got contams? [Re: tchyted]
    #796803 - 08/04/02 10:25 PM (21 years, 7 months ago)

hehe my bud has nitrous remote control cars we flamed with nitrous worked really well hehe but id flame no matter what..............mho

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Offlinekykeon
Dead wishes

Registered: 05/30/02
Posts: 1,506
Loc: A universe right next to ...
Last seen: 17 years, 5 months
Re: Is this why all my jars got contams? [Re: fearforce7]
    #797026 - 08/05/02 04:34 AM (21 years, 7 months ago)

A lot of things may lead to contams actually and as i have experienced myself, there is no standard procedure as long as you dont have the same conditions. Which means that i had twice contaminated jars and then i found what was going wrong. I was even wearing gloves and mask, not to mention flaming, dettoling etc, but i was failing. I was having a very bad time because i thought i wasnt sterilized my teks enough. Always saying, is it the needle? is it the spores? is it the pc? is it the grains?

Then i realized that most people in here that i was following their teks was from northern europe or northern states, so i changed the place i stored my jars. Voila! Success, with the exact method. The cupboard i used to keep them was going extremely hot because it was in the f***** kitchen. I moved them to another room, inside a cardboard where the temp was lower and i succeded. Why? Because TOO HIGH temperature, above some levels of say 40 C, make spores hard to start their cycle, while bacteria were having a party. And they smell so bad...

Just my experience. It doesnt mean this is the answer 4 you. My point is perhaps you should seek the answer somewhere you dont even think of.

on the other side, it wouldnt hurt to be more sterile, would be?

Peas lol

Wyckeon
athens, greece


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The living ghost of Kykeon

Edited by kykeon (08/05/02 04:35 AM)

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Anonymous

Post deleted by Moe Howard [Re: fearforce7]
    #797806 - 08/05/02 11:37 AM (21 years, 7 months ago)


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Offlinerheidiohed121
Simeon

Registered: 06/19/02
Posts: 157
Last seen: 20 years, 10 months
Re: Is this why all my jars got contams? [Re: ]
    #797822 - 08/05/02 11:43 AM (21 years, 7 months ago)

Lysol. Use tons and tons of Lysol. It is a life saver or shrooms saver if you want to look at it like that.


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i apologize if i aide in the spreading of any misinformation

"Adventavit asinus,
Pulcher et fortissimus"

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OfflineI_Fart_Blue
Stranger

Registered: 06/25/02
Posts: 3,495
Loc: SItting on the Group W Be...
Last seen: 20 years, 2 months
Re: Is this why all my jars got contams? [Re: ZEPH]
    #797865 - 08/05/02 11:58 AM (21 years, 7 months ago)

"hehe my bud has nitrous remote control cars we flamed with nitrous worked really well hehe " No you didn't. Quit lying.


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"A study of the history of opinion is a necessary preliminary to the emancipation of the mind. I do not know which makes a man more conservative-to know nothing but the present, or nothing but the past." -John Maynard Keynes

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Offlinewiser
member
Registered: 04/23/02
Posts: 162
Loc: countryside
Last seen: 20 years, 5 months
Re: Is this why all my jars got contams? [Re: I_Fart_Blue]
    #798114 - 08/05/02 02:26 PM (21 years, 7 months ago)

This is my first grow without any contamz. I just did it all in my bathtub. Cleaned the bathroom including the bathtub (with dettox (english equivalent to lysol i geuss)) and flame the needle after each innoculation. Wore dustmask, gloves, bag over hair, etc...
Worked good...
lataz


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I like to SHIMMY to my locations....

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Offlinewiser
member
Registered: 04/23/02
Posts: 162
Loc: countryside
Last seen: 20 years, 5 months
Re: Is this why all my jars got contams? [Re: wiser]
    #798116 - 08/05/02 02:27 PM (21 years, 7 months ago)

OOPs...
I meant this is my first grow and i DONT have any contams, not my first grow with no contams...
tired...


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I like to SHIMMY to my locations....

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Invisiblejokefox
Top of the chain
Male User Gallery


Registered: 12/22/09
Posts: 6,231
Loc: never where I should be
Re: Is this why all my jars got contams? [Re: wiser]
    #11715503 - 12/25/09 02:58 AM (14 years, 3 months ago)

Quote:

wiser said:
This is my first grow without any contamz. I just did it all in my bathtub. Cleaned the bathroom including the bathtub (with dettox (english equivalent to lysol i geuss)) and flame the needle after each inoculation. Wore dustmask, gloves, bag over hair, etc...
Worked good...
lataz 




like a safeway bag lol jk

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