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Offlinesacredcow
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Registered: 08/01/09
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Last seen: 13 years, 12 days
Help a noob confirm that this is trich?
    #11666616 - 12/17/09 04:44 AM (14 years, 2 months ago)

I am pretty sure it is. It wasn't there yesterday, now the whole jar is white basically with green spots.
Luckily only seeing it in one of 12 BRF jars




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Offlinesacredcow
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Registered: 08/01/09
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Loc: TN
Last seen: 13 years, 12 days
Re: Help a noob confirm that this is trich? [Re: sacredcow]
    #11666635 - 12/17/09 04:57 AM (14 years, 2 months ago)

I underestimated the size of the syringe needle when preparing so many of the holes i made in the jar lids were too small for proper inoculation. Most of the jars had at least one or two holes the needle would fit through so i just inoculated them per usual through the holes and then through the two coffee filters i have between the lid and the verm layer, however a couple jars such as this one had no holes of suitable size for inoculation whatsoever and had to be opened for open air injection of spores through the verm layer. That's probably where this one most likely picked up the contam, but luckily as the coffee filers between the lid and verm layer weren't punctured, they're keeping the trich spores from getting out and contaminatng everything else.


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Offlinesacredcow
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Re: Help a noob confirm that this is trich? [Re: sacredcow]
    #11666745 - 12/17/09 06:22 AM (14 years, 2 months ago)

So can anyone help me confirm this?


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Offlinesacredcow
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Re: Help a noob confirm that this is trich? [Re: sacredcow]
    #11667827 - 12/17/09 11:49 AM (14 years, 2 months ago)

could also be aspergillus or something?
sorry for so many thread bumps
just wanting some advice


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Offlinesolumvita
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Re: Help a noob confirm that this is trich? [Re: sacredcow]
    #11674222 - 12/18/09 10:15 AM (14 years, 2 months ago)

Not sure about the trich identity, it certainly needs to be tossed, if you really want when you throw it away smell it, trich smells quite 'earthy'.

most likely it is trich given that you opened the lid.  It does mena that you need to clean the room you work in.  wash the walls/floors/everywhere you can reach, down with bleach (10%) you might want to open the windows first as it can get quite smelly.


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OfflineRansom
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Re: Help a noob confirm that this is trich? [Re: solumvita]
    #11689315 - 12/20/09 10:22 PM (14 years, 1 month ago)

Dont forget to pressure cook, or wash with bleach.


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Offlineveda_sticks
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Re: Help a noob confirm that this is trich? [Re: Ransom]
    #11701843 - 12/22/09 07:24 PM (14 years, 1 month ago)

dont know why you are using coffee filters, which contaminant spores have an easy job getting trhough since the holes in coffee filters are 10X the size.

Your dry verm layer is your contamination barrier.

I never recomend open air inoculations.


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OfflineSuntzu
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Re: Help a noob confirm that this is trich? [Re: sacredcow]
    #11702618 - 12/22/09 09:12 PM (14 years, 1 month ago)

I vote either Penicillium or Aspergillus. . .impossible to differentiate between these two without a scope, and very difficult to verify with a scope without spreading the spores everywhere.  I think Trich is too-often blamed in grain contamination, and that more often it's either P or A [at least as far as green molds go].  Trich is much happier in peat/soil, i.e. casing mixes, than P or A are.


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