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OfflineSporeGirl000
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Registered: 09/09/09
Posts: 260
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Last seen: 10 years, 3 months
N00b girl here, is everything I'm doing alright so far?
    #11693369 - 12/21/09 04:13 PM (15 years, 30 days ago)

Hey just wanted some conformation that everything I'm doing is alright so far. If I did anything wrong please correct me!


1st-filled 12 1/2 pint regular mouth jars with pf's brf/verm mix.

2nd-filled 12 1/2 jars with wbs that i soaked for 15 hrs and let drain for 5. it felt moist but not at all drippy or soggy. (I'm attempting to do both cakes and bulk spawn)

3rd-put tyvek on all lids since it is supposed to block out contamination. I thought maybe better safe then sorry.
note: All libs have 4 holes punched in the top for inoculation, I hope its alright for the wbs. please let me know if its not..I wanted to do the 2 poly fill holes but the drill i bought is apparently a piece of shit and i couldnt get holes large enough to fit in a wad of polyfill.

4th-as I have said the jars are being pressure cooked right now at 15psi and I'm doing this for 90 minutes.

please let me know if all this sounds good or doesnt sound so good!

thanks

-Sg


*Update*

I inoculated the jars, after waiting 6 hours for them to cool.  I can only describe the attempt as an very awkward learning experience.

I made a nice still air glove box which I have been really excited about, only when it came to actually using it i found the arm holes in it were too close together making properly inoculating the jars pretty much impossible. in the first jar i injected a good chunk (about a 4th) into the damn hole.
so after that I decided i didnt really have much of a choice but to knock up the rest outside my glovebox of fail.

I kept everything as sterile as possible,  -lots of alcohol wipes, gloves and flames and patching the holes with micropore tape.

I've had a true hepa air filter running nonstop for a while so hopefully the air is clear.

So I'm less then pleased with myself at the moment but I'm keeping my fingers crossed that everything works out okay.

Thanks again for everyones advice.

Sporegirl.

Quick question: my needle ran dry so I had an extra jar of wild bird seed that I poured out. I found it was sort of on the dry side with moisture more on the top and bottom.  I read that too dry is better then too wet but I'm worried that the rest of the jars might not be wet enough to support mycilium.  What do you think?


--------------------
Anyway, no drug, not even alcohol, causes the fundamental ills of society.  If we're looking for the source of our troubles, we shouldn't test people for drugs, we should test them for stupidity, ignorance, greed and love of power.  ~P.J. O'Rourke  :mushroom2:

Edited by SporeGirl000 (12/22/09 12:39 AM)

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Offlineshroom239
aka K.T.
Male


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Registered: 08/12/09
Posts: 550
Last seen: 9 years, 9 days
Re: N00b girl here, is everything I'm doing alright so far? [Re: SporeGirl000]
    #11693393 - 12/21/09 04:16 PM (15 years, 30 days ago)

It might be rough to get cakes out of a regular mouth jar, but other than that looks good. :thumbup:


--------------------
Disclaimer: Any picture or statement I post here is from research I do on my free time, I do not actually partake in this hobby.

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OfflineSporeGirl000
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Registered: 09/09/09
Posts: 260
Loc: The South Flag
Last seen: 10 years, 3 months
Re: N00b girl here, is everything I'm doing alright so far? [Re: shroom239]
    #11693535 - 12/21/09 04:37 PM (15 years, 30 days ago)

Yeah I tried finding wide mouth but they only came in quarts.  :<

Ill probably order wide mouth from on line next time.


--------------------
Anyway, no drug, not even alcohol, causes the fundamental ills of society.  If we're looking for the source of our troubles, we shouldn't test people for drugs, we should test them for stupidity, ignorance, greed and love of power.  ~P.J. O'Rourke  :mushroom2:

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InvisibleTacoHerder
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Registered: 06/10/09
Posts: 10,107
Re: N00b girl here, is everything I'm doing alright so far? [Re: shroom239]
    #11693540 - 12/21/09 04:38 PM (15 years, 30 days ago)

all looks good. the reg mouth jars will be fine, just makes for slower colonization. pc for 60 min should be long enough. and no need for 4 holes on the wbs jars, but it wont hurt. good luck:super:


--------------------
TO ALL CULTIVATORS, EVERYTHING YOU NEED TO KNOW http://www.shroomery.org/forums/showflat.php/Number/10899385
We need more member like mycochef, he is truly a kind giving person. Just ask Noobie, mycochef hooked him up big time!
Fastest Cultivation WINNER!!!! NoOneKnowsHowToAct won it twice, must be doing something right:super:

Shoot me a pm if you would like a powerful custom laser built. :smile: 4th pic is a 1.3W 445nm laser burning thru some plastic makeup containers. Yes I was wearing proper safety goggles, otherwise I would be blind right now.

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Offlineoxalic32
Male
Registered: 01/27/08
Posts: 3,615
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Last seen: 13 years, 8 months
. [Re: SporeGirl000]
    #11694319 - 12/21/09 06:37 PM (15 years, 29 days ago)

.

Edited by oxalic32 (12/19/10 01:33 PM)

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InvisibleTacoHerder
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Registered: 06/10/09
Posts: 10,107
Re: N00b girl here, is everything I'm doing alright so far? [Re: oxalic32]
    #11694592 - 12/21/09 07:19 PM (15 years, 29 days ago)

Quote:

oxalic32 said:
I don't think you'll be getting a full cake out of your regular mouth jars. I would plan to spawn to bulk, that's what i did when i realized i had to.

The tyvek isn't necessary for PF tek. In fact it might just take away gas exchange, you could always remove it down the road.

If you're going to work with grains i would get some quart jars down the line. 1/2 pints jars of wbs aren't worth dealing with in my books.

So far so good. Also look for a bigger drill bit for your drill so you can make grain jars with 1 poly fill hole.





Ive done Pf tek a number of time with 100% colonization using the 1/2 pint reg mouth jars, but sometimes they may stall and need to be turned upside down to help them finish. Also the substrate has the tendency to compact a little too much and not allow the mycl to colonize that part also. Just birth and cut that part out if necessary. the tyvek isn't necessary but it wont harm. And using 1/2 pint jars for wbs works better in my opinion. You can start out with a few 1/2 pint jars, since they will colonize faster than the quart you will be able to do some g2g to qt jars after that. Saving allot of time over waiting for the qt jar to colonize 100% then do g2g.


--------------------
TO ALL CULTIVATORS, EVERYTHING YOU NEED TO KNOW http://www.shroomery.org/forums/showflat.php/Number/10899385
We need more member like mycochef, he is truly a kind giving person. Just ask Noobie, mycochef hooked him up big time!
Fastest Cultivation WINNER!!!! NoOneKnowsHowToAct won it twice, must be doing something right:super:

Shoot me a pm if you would like a powerful custom laser built. :smile: 4th pic is a 1.3W 445nm laser burning thru some plastic makeup containers. Yes I was wearing proper safety goggles, otherwise I would be blind right now.

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OfflineRailrider
Chronic contaminator


Registered: 07/10/09
Posts: 598
Loc: a van down by the river.
Last seen: 2 months, 5 days
Re: N00b girl here, is everything I'm doing alright so far? [Re: TacoHerder]
    #11695331 - 12/21/09 09:28 PM (15 years, 29 days ago)

I put tyvek(thin type) filter on all my jars no matter what the spawn type after inoculation.I haven't had a contaim since I started doing it.I imagine at some point you will ditch the 1/2 pinters,start looking at the quart jars for grains.GL


--------------------

Any post I post here is pure speculation from other read posts
Best tip for cultivation!

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InvisibleTacoHerder
Bluedavenger
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Registered: 06/10/09
Posts: 10,107
Re: N00b girl here, is everything I'm doing alright so far? [Re: Railrider]
    #11695371 - 12/21/09 09:32 PM (15 years, 29 days ago)

Quote:

Railrider said:
I put tyvek(thin type) filter on all my jars no matter what the spawn type after inoculation.I haven't had a contaim since I started doing it.I imagine at some point you will ditch the 1/2 pinters,start looking at the quart jars for grains.GL




I do use the qt jars for grain. but i start my master jars using 1/2 pint jars. they colonize 4 times faster(since a qt. has 4 1/2 pints). then i prep some qt grain jars and do some g2g. I usually get 3 qt jars out of 1 1/2 pint jar of colonized wbs.


--------------------
TO ALL CULTIVATORS, EVERYTHING YOU NEED TO KNOW http://www.shroomery.org/forums/showflat.php/Number/10899385
We need more member like mycochef, he is truly a kind giving person. Just ask Noobie, mycochef hooked him up big time!
Fastest Cultivation WINNER!!!! NoOneKnowsHowToAct won it twice, must be doing something right:super:

Shoot me a pm if you would like a powerful custom laser built. :smile: 4th pic is a 1.3W 445nm laser burning thru some plastic makeup containers. Yes I was wearing proper safety goggles, otherwise I would be blind right now.

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OfflineRailrider
Chronic contaminator


Registered: 07/10/09
Posts: 598
Loc: a van down by the river.
Last seen: 2 months, 5 days
Re: N00b girl here, is everything I'm doing alright so far? [Re: TacoHerder]
    #11695585 - 12/21/09 10:01 PM (15 years, 29 days ago)

Well i went a bit gun ho with buying half pints when I got into the hobby and bought around 48 or so :grin:

Now if I make some spawn I do quarts and use  wide mouth pints for Grain to grain.

I still do pf tek for fun once in a while cause I like to watch the myc. grow but I have waaayyy to many 1/2 pints around.

I figured if i ever got into agar work they would come in handy but other thatn that they just sit in a box.


--------------------

Any post I post here is pure speculation from other read posts
Best tip for cultivation!

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InvisibleTacoHerder
Bluedavenger
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Registered: 06/10/09
Posts: 10,107
Re: N00b girl here, is everything I'm doing alright so far? [Re: Railrider]
    #11695625 - 12/21/09 10:04 PM (15 years, 29 days ago)

Quote:

Railrider said:
Well i went a bit gun ho with buying half pints when I got into the hobby and bought around 48 or so :grin:

Now if I make some spawn I do quarts and use  wide mouth pints for Grain to grain.

I still do pf tek for fun once in a while cause I like to watch the myc. grow but I have waaayyy to many 1/2 pints around.

I figured if i ever got into agar work they would come in handy but other thatn that they just sit in a box.




u can use them to store your spore prints. fill it up with some distilled water. PC for 15 min. then drop inthe whole spore print. when you want some just suck some up with a needle.


--------------------
TO ALL CULTIVATORS, EVERYTHING YOU NEED TO KNOW http://www.shroomery.org/forums/showflat.php/Number/10899385
We need more member like mycochef, he is truly a kind giving person. Just ask Noobie, mycochef hooked him up big time!
Fastest Cultivation WINNER!!!! NoOneKnowsHowToAct won it twice, must be doing something right:super:

Shoot me a pm if you would like a powerful custom laser built. :smile: 4th pic is a 1.3W 445nm laser burning thru some plastic makeup containers. Yes I was wearing proper safety goggles, otherwise I would be blind right now.

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OfflineRailrider
Chronic contaminator


Registered: 07/10/09
Posts: 598
Loc: a van down by the river.
Last seen: 2 months, 5 days
Re: N00b girl here, is everything I'm doing alright so far? [Re: TacoHerder]
    #11695675 - 12/21/09 10:11 PM (15 years, 29 days ago)

I got 3 GT prints in some jars with/out water in my house somewhere I can't find lol.I think that sporegirl is doing fine tho.


--------------------

Any post I post here is pure speculation from other read posts
Best tip for cultivation!

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Offlineoxalic32
Male
Registered: 01/27/08
Posts: 3,615
Loc: .
Last seen: 13 years, 8 months
. [Re: Railrider]
    #11696040 - 12/21/09 11:07 PM (15 years, 29 days ago)

.

Edited by oxalic32 (12/19/10 01:33 PM)

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OfflineSporeGirl000
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Registered: 09/09/09
Posts: 260
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Last seen: 10 years, 3 months
Re: N00b girl here, is everything I'm doing alright so far? [Re: oxalic32]
    #11696582 - 12/22/09 12:42 AM (15 years, 29 days ago)

Update up top!!


--------------------
Anyway, no drug, not even alcohol, causes the fundamental ills of society.  If we're looking for the source of our troubles, we shouldn't test people for drugs, we should test them for stupidity, ignorance, greed and love of power.  ~P.J. O'Rourke  :mushroom2:

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Offlinehermione_granger
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Registered: 12/26/09
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Re: N00b girl here, is everything I'm doing alright so far? [Re: SporeGirl000]
    #11725390 - 12/27/09 10:22 AM (15 years, 24 days ago)

I would stick with brf/verm for such small jars. When you're starting out, it's easiest and gives you the best results anyway. I've made up several batches of these types of cakes and they won't produce a ton of fruits, they are an excellent starting point.

Just curious: did you put a verm barrier on the brf cakes? A .5" layer of small particle verm will give you the best barrier against contams. (I've used the larger particle stuff...between .25" to .5" pieces...and it doesn't seem to create enough of a shield).

And how are things growing?

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Offlinesacredcow
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Registered: 08/01/09
Posts: 64
Loc: TN
Last seen: 13 years, 11 months
Re: N00b girl here, is everything I'm doing alright so far? [Re: hermione_granger]
    #11725625 - 12/27/09 11:24 AM (15 years, 24 days ago)

I take it you're using multispore? Suggestion: use liquid culture
If you're dealing with such large amounts anyway, I'd use lc. It's generally accepted to reduce colonization time by 1-2 weeks and is relatively easy to start.

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