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InvisibleChe_Night_Soil
Koinoniphobic

Registered: 07/16/02
Posts: 1,533
Loc: Commonslaughtia
Bacillus mysterious
    #1167657 - 12/24/02 01:43 PM (14 years, 3 months ago)

I thought I should post this here in the Contam. forum too: after soaking grain for 24 hrs, rinsing and draining, pressure-cooking for an hour and 15 min., shaking and inoculating under very clean conditions, how is Bacillus going to show up and fuck up all kind of plans  :mad:. The jars haven?t stalled, despite the seeming bacteria problem, and one of them is almost fully there. This may be just stupid, but is there any way to kill the Bac. without harming the myc.? Thaks in advance for any help. 


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if(human==autoPart){
for(i=1;i<infinity;i++){
getBorn(mind,soul);
getEdu(mind,soul);
getJob(mind,soul);
retire(mind,soul);
die(mind,soul);
}
}


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Anonymous

Post deleted by Administrator [Re: Che_Night_Soil]
    #1167845 - 12/24/02 03:30 PM (14 years, 3 months ago)



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InvisibleTinMan
Stranger

Registered: 10/01/02
Posts: 2,956
Loc: Russia
Re: Bacillus mysterious [Re: ]
    #1170777 - 12/26/02 05:55 AM (14 years, 3 months ago)

If theres one contam I hate the most its that... and cobweb. I've had numerous jars attacked by it when they were 95% complete. The bleach dunking thing sounds like it will work, so next time I won't toss the jars right away.


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OfflineSeussA
Error: divide byzero

Folding@home Statistics
Registered: 04/27/01
Posts: 23,480
Loc: Caribbean
Last seen: 2 months, 22 days
Re: Bacillus mysterious [Re: Che_Night_Soil]
    #1175096 - 12/28/02 11:24 AM (14 years, 3 months ago)

Make sure that your incubator is clean. Bacillus are very mobile bacteria and can travel an amazing distance. Next time your incubator is empty, wipe it down with a bleach/water solution and let it dry. Then use hot soap and water, followed by a strong alcohol solution. Don't forget any heater elements, etc. When bacillus forms endospores, you are usually left with a dry white film that is very difficult to remove. When wet and mobile, the bacteria is usually a grey/white mucus that is very sticky.


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Just another spore in the wind.


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InvisibleChe_Night_Soil
Koinoniphobic

Registered: 07/16/02
Posts: 1,533
Loc: Commonslaughtia
Re: Bacillus mysterious [Re: Seuss]
    #1176142 - 12/28/02 10:09 PM (14 years, 3 months ago)

Thank you all for the advice. I will clean my incubator for sure. Has anyone had success with bleach-dunking jars of grain?


--------------------
if(human==autoPart){
for(i=1;i<infinity;i++){
getBorn(mind,soul);
getEdu(mind,soul);
getJob(mind,soul);
retire(mind,soul);
die(mind,soul);
}
}


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