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noobieshroomie
Back again




Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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Re: The things people do for their grow that are a waste of time/money [Re: 13shrooms]
#11683811 - 12/19/09 11:00 PM (15 years, 1 month ago) |
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pretty much
there are a few intelligent answers in here but not many
-noobie-
-------------------- AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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beatnicknick
The Innovator



Registered: 05/25/05
Posts: 1,074
Last seen: 13 years, 8 months
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Re: The things people do for their grow that are a waste of time/money [Re: noobieshroomie]
#11683946 - 12/19/09 11:42 PM (15 years, 1 month ago) |
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When in doubt, listen to double R
-------------------- I don't think for myself. I think as though I'm explaining my thoughts to someone else. I'm concerned only for those listening.
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artizen
JEFFERSONIAN



Registered: 12/16/08
Posts: 1,996
Loc: HOME SWEET HOME
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Re: The things people do for their grow that are a waste of time/money [Re: RogerRabbit]
#11685098 - 12/20/09 09:08 AM (15 years, 1 month ago) |
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Quote:
RogerRabbit said:
Quote:
tugwax said: Here's the beginnings of list of things that I've read in the forum that aren't really necessary or involves excessive expense. Please add your own!
Trying to find lights with just the right color temperature.
Agreed, provided that a 50% or better increase in harvest weight for the next five or six years isn't worth a couple of quid/$3 for the correct spectrum lamp. For most of us, the payoff on buying a natural daylight fluorescent or LED lamp comes on the very first harvest.
Quote:
tugwax said: Automatic moisture delivery or FAE for a small grow. I myself fell into this OTT solution for just 9 cakes. Even though it was successful I soon reverted to manual methods with greater yield. Now the timers, air pump and fogger are gathering dust, £70 wasted.
Agreed. Been saying it for years regarding terrariums. Save the humidifiers and timers for a mini-greenhouse.
Quote:
tugwax said: Trying to find hardwood chips when any old mixed stuff will do.
Not so. While softwoods will colonize faster because they're less dense, fruiting performance is substantially reduced. Mixed woods are OK if the majority of material comes from hardwood sources, with just a few conifers tossed in. If you want to use Pine, chip it up and let it sit in the sun for a year before use. However, it will still underperform hardwood. RR
even this was putting it lightly. i thought he was gonna on this one, for sure.
if you need to save money this bad just don't bother getting into mycology. wtf experimentation is part of the life energy of this place and this hobby. if your a begginer, do a couple of brf cakes in a milk jug. if you get hooked on the hobby, you'll find the money to do things properly. it's one thing to say "whatever works for YOU" i completely agree with that but, most of the suggestions in this thread are simply lazy, cheapskate, cornercutting and just BAD ADVICE.
if you wanna save money go here http://www.shroomery.org/forums/showflat.php/Number/9107974#9107974 or ebay or craigslist or walmart but don't just NOT BUY THINGS. in general we don't NEED anything, we could all just sit arround a fucking candle every night.

ok, i'm done. 
bitches
--------------------

HCA AMU
IN LOVING MEMORY
1.6.1917 - 4.3.2010
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tugwax
Anomaly



Registered: 11/12/09
Posts: 160
Loc: Little Britain
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Re: The things people do for their grow that are a waste of time/money [Re: artizen]
#11686264 - 12/20/09 01:10 PM (15 years, 1 month ago) |
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It would have been nice if more experienced growers had added some things that they view as a waste in order to steer the new growers into making the most of their pocket money and away from worn shoe leather.
The topic has been lost in the noise of nit-picking, wallet ego and controversy.
Will a Moderator please lock it?
-------------------- Freedom is as great as one's tolerance for ambiguity.
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artizen
JEFFERSONIAN



Registered: 12/16/08
Posts: 1,996
Loc: HOME SWEET HOME
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Re: The things people do for their grow that are a waste of time/money [Re: tugwax]
#11686568 - 12/20/09 02:00 PM (15 years, 1 month ago) |
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Quote:
tugwax said: It would have been nice if more experienced growers had added some things that they view as a waste in order to steer the new growers into making the most of their pocket money and away from worn shoe leather.
The topic has been lost in the noise of nit-picking, wallet ego and controversy.
Will a Moderator please lock it?
OK
cold shocking pre-made spawn/sub wick type humidifiers any incubator
i think jenshot mentioned the TIT and there were one or 2 other suggestions that made sense but, when nearly every other post is saying not to follow commonly practiced and PROVEN methods like a GB and casing layers and suggesting alternitives like the oven door thing, wtf.
i do agree with one thing
i'm sorry i even bumped it with my rants but, hopefully this will keep noobs from taking bad advice to save some pocket change.
this is not a personnal attack. i'm sure you had the best intentions.
--------------------

HCA AMU
IN LOVING MEMORY
1.6.1917 - 4.3.2010
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sleepyrz
Strangerwithcandy
Registered: 12/19/09
Posts: 35
Last seen: 4 years, 6 months
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Re: The things people do for their grow that are a waste of time/money [Re: artizen]
#11688545 - 12/20/09 07:47 PM (15 years, 30 days ago) |
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my favorite was the guy who pressure cooks his jars empty
then takes them out and opens them to cool.......................................................
to then fill them to pressure cook them again
really? seriously? are you that impaired that you don't realize what you are doing is retarded and a complete, and i mean total....waste of time
and these incubators and glove boxes an incubator is useful if you live outside in a tent in canada and the only thing i can see a glovebox useful for is g2g or maybe making a spore syringe
everything else can be done with an alcohol soaked sponge on the needle no gloves no cleaning no retarded mask and hair net
RTV + alcohol soaked sponge on needle = win
i find it slightly saddening that they don't spend the time to research and find that there are better ways to do things
they read one thing once and think that's all there is
no, its a constant improvement process like everything in life
but i guess there are people out their who are good with status quo and being mediocre
i at least will take the time to attempt improvement
remember without improvement we would all most likely be doing pf jars
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WHEN IT RAINS IT SHROOMS
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Lucid_Euphoria
Sojourner


Registered: 03/23/05
Posts: 1,045
Loc: Flip Side
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Re: The things people do for their grow that are a waste of time/money [Re: sleepyrz]
#11690427 - 12/21/09 02:48 AM (15 years, 30 days ago) |
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-------------------- PAN CYAN & AZURE FOR TRADE
"If ignorance is bliss, THEN KNOCK THE SMILE OFF MY FACE!" - Zach de la Rocha
"Today a young man on acid realized that all matter is merely energy condensed to a slow vibration, that we are all one consciousness experiencing itself subjectively, there is no such thing as death, life is only a dream, and we are the imagination of ourselves. Here's Tom with the weather!" -Bill Hicks-
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VespertineDweller
Virgin



Registered: 11/23/09
Posts: 19
Last seen: 13 years, 5 months
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Re: The things people do for their grow that are a waste of time/money [Re: Lucid_Euphoria]
#11690445 - 12/21/09 03:00 AM (15 years, 30 days ago) |
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First time through im regretting even doing pf tek. spawning some cheep seed to coir is my next bet.
-------------------- "By engaging mycelium we can change the world" -Paul Stamets
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Dragonaut


Registered: 06/24/04
Posts: 6,487
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Re: The things people do for their grow that are a waste of time/money [Re: Lucid_Euphoria]
#11690461 - 12/21/09 03:10 AM (15 years, 30 days ago) |
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#1 waste of time...using the search engine. Just make a post and expect everyone to come up with answers for you. With any luck, it will work.
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Cloneufc
Master Exploder!



Registered: 11/15/07
Posts: 1,237
Loc: Las Vegas
Last seen: 1 year, 5 months
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Re: The things people do for their grow that are a waste of time/money [Re: Dragonaut]
#11690575 - 12/21/09 04:38 AM (15 years, 30 days ago) |
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Drum Roll Please! Open air inoculation is the biggest waste of time. I put this together because its so damn funny.
"1 cubic foot of normal household air contains on average: 100,000 invisible particulates. So, every square inch of that air carries: One hell of a load of possible contaminate spores. Agar
Best success rate for open air inoculation. Is to continuously keep the needle covered. With an alc saturated gauze pad, foam, paper towel, or cotton balls. Otherwise, you will not BE LUCKY, FOR LONG." Agar
Anyone who recommends open air inoculations can be compared to a drunk who recommends drinking and driving. RR
Agar is a very experienced grower. His method you linked keeps the needle covered at all times in an alcohol soaked cloth. That's actually not 'open air'. Leave the needle in open air and see what happens. A glovebox is very easy to make. RR
dgls_jms
"id say a glovebox is a waste considering i never use one and rarely if ever get a contamination
I agree, I do all my work on a cookie sheet, door open to a hot oven. Is a little warm but hasent fail me yet."
"so I had a WBS jar that started showing contams a couple days after the myc started growing. I put it on my back porch and forgot about it. Now I'm not going to use but but the battle for life inside that jar has been amazing to watch. The myc pushed the mold to the very top, just a very few grains, and took over the whole jar. Fun to watch!"
"I ordered up 4 syringes from ralph. I also ordered a "sterile liquid culture" in a qt mason jar off of ebay. They ebay dude was taking forever to ship so I went out and bought some baby bottles, did my best to sterilize in the mic, knocked up after they cooled down. 30 hours later, mycelia is covering the bottom, so I swirled to distribute it throughout the culture. After I swirled, they became cloudy and remained that way, never saw the mycelia back at the bottom. This is when I realized I should invest in a pc, so I ordered one up, should arrive today. So while I'm waiting for my pc, my "sterile lc" arrived. It was clear and apeared to be contan free. knocked it up with a cc from a fresh spore syringe. 24 hours later, bottoms covered in mycelia. So last night, I swirled to disrtibute it. This morning the jar is all cloudy, and there is not as much mycelia on the bottom as last night. I am starting to think it is either ralphs syringes, or the "sterile aiport syringe" that this guy sent me. I figured when he said sterile it would still be in the autoclave bag, but it was a syringe stuffed with polyfill, no plunger, with the needle already attached, whole think rapped in foil, in a ziplock bag. I used ipa and flamed the needles. Also, It got really could here over the past week, went from 80's to around 70, dropping into the 50's at night. MY thermostat reads 70 inside, but I think it might get lower during the night, as the heater is not turned on yet. I know this would slow myclia growth, but would it speed up bacterial growth? I was thinking about setting the jar on a heating pad thing, but I don't want it to get too hot. Any help would be greatly appreciated. Thanks"
sHrOoMaCiDe
"On one of my recent attempts, i used 6 BRF/Verm jars and cubensis spore syringes. I inoculated in open air(dumb idea) all six jars about 3 weeks ago. The result: 4jars infected with green/black molds, and the other 2 surviving jars did not colonize or change at all. No growth. What happened? I inoculated 1-2 maybe even 3cc's in each jar. Still after 3 weeks, nothings happened. Any insights for me? Seems like failure. Really disappointing ya know?"
I got extremely lucky with innoculating my LC and fungi rye bag in open air. They are both progressing without contamination. However, the bag is progressing at a glacial speed. The LC is almost done colonizing. When its done should I suck up some of that mycelium and inject it into my fungi bag to speed it up?
I've never had success in growing mushrooms yet, but my 3rd attempt was the closest. I inoculated two quart sized pre-sterilized rye grain jars back in july. The mycelium grew in one of them but not the other. However, it grew about halfway up the jar and then stopped. It went dormant or something. so my question is, can i re-inoculate the jar that didn't grow any mycelium? its really old, but theres no contamination.
VampireSlayer Why i'm done with OPEN AIR INOCULATIONS
"Just wanted to say...i did open air innoc for a year with pretty good success. actually only till the last couple months have I been getting contams...I do not fuck with open air period!! it is just a 50/50 in my oppinion. I put the blame on a vendor here about a contammed syringe but NOW i'm not sure if it was the the vendor to blame or if it was me...the point is if you use any other method (flow air or still air) then you can easssily put the blame on the culture or syringe (talking cubie syringer growers here not graintograin and stuff) but grain to grain I WOULD NEVER do in open air but NOW i'll never even do open air period...it's too risky period"
NinjaGaiden
"I've had 100% success with using the oven tek. I do everything: Make syringes, inoculate jars, G2G transfers using the oven.
I'm not at all trying to debate if the tek itself is bogus or not, but it does work for some." NinjaGaiden
"more like 100% LUCK
and I will debate it...
the Oven tek is crap!~" Roadkill
jeetered
"i always open air inoculate. I like "glacial" that's great, i've found that with spore to grain before too. LC to grain is much much faster. I don't know anything about adding LC to it to speed it up though."
"i do pretty much everything open air except agar work."
Re: What in the world! Contamination Forum jeetered 03/25/07 06:58 PM Re: Contamination??? Contamination Forum jeetered 05/17/07 08:43 PM Re: Contamination??? Contamination Forum jeetered 05/18/07 07:51 PM Re: Bacillus contam in rye Contamination Forum jeetered 07/01/07 09:47 AM black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/07/07 08:08 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/07/07 08:34 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/07/07 10:44 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/07/07 10:51 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/08/07 05:37 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/09/07 05:31 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/10/07 07:54 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum
figgusfiddus
"Yeah, open air inoculation isn't really that risky. When I get screwed up, it's usually the substrate conditions that do it."
Re: Contamination? Contamination Forum figgusfiddus 05/10/07 01:15 PM Re: Contamination? Contamination Forum figgusfiddus 05/14/07 10:39 AM Re: Daaaaaaaamn! Green Mold... Contamination Forum figgusfiddus 06/13/07 06:53 PM Re: weird growth on cake (pic) Contamination Forum figgusfiddus 06/16/07 05:05 PM Re: weird growth on cake (pic) Contamination Forum figgusfiddus 06/16/07 08:28 PM Re: What kind of infection is this? [pics] Contamination Forum figgusfiddus 06/16/07 08:50 PM Re: What kind of infection is this? [pics] Contamination Forum figgusfiddus 06/16/07 08:57 PM Re: Daaaaaaaamn! Green Mold... Contamination Forum figgusfiddus 06/17/07 02:19 PM Re: weird growth on cake (pic)update Contamination Forum figgusfiddus 06/17/07 06:13 PM Re: wet looking caps Contamination Forum figgusfiddus 06/17/07 06:15 PM Re: weird growth on cake (pic) Contamination Forum figgusfiddus 06/18/07 02:49 PM Re: ...is this Trich? Contamination Forum figgusfiddus 07/06/07 01:27 PM Re: ...is this Trich? Contamination Forum figgusfiddus 07/06/07 01:28 PM Low humidity promoting molds (esp. trichoderma)? Contamination Forum figgusfiddus 07/28/07 04:13 PM Re: Low humidity promoting molds (esp. trichoderma)? Contamination Forum figgusfiddus 07/30/07 10:27 PM Re: If i have a piece of bread with green mold on it... Contamination Forum figgusfiddus 01/30/08 03:46 PM
myndreach
"do most people here actually use a glovebox or something? SWIM has never had contams as a result of air...the one they got was because of a bad syringe.
lysol for surfaces, oust for the air, dust mask and rubber gloves."
Re: Worst Contam Pic Contamination Forum myndreach 05/27/05 08:55 AM Re: tiny black spec on mycelium Contamination Forum myndreach 06/01/05 04:26 PM First grow...AFOAF is freaking out...come this far....contam? Contamination Forum myndreach 06/10/05 09:27 PM Re: First grow...AFOAF is freaking out...come this far....contam? Contamination Forum myndreach 06/10/05 09:50 PM Re: black stuff in syringe Contamination Forum myndreach 06/11/05 10:48 PM Re: Possible contam on mushrooms? Contamination Forum myndreach 06/12/05 08:22 PM Re: brown mycellium? Contamination Forum myndreach 06/12/05 08:23 PM Re: spots on white mycelium Contamination Forum myndreach 06/13/05 09:00 AM Contam or Myc Piss? Contamination Forum myndreach 06/13/05 11:06 AM Re: Contam or Myc Piss? Contamination Forum myndreach 06/14/05 07:27 AM Contams in jar? Brown/Orange/Wet spot mold? Need help quick please. Contamination Forum myndreach 07/02/05 09:51 AM Re: Question about appearance of some recently acquired dried shrooms Contamination Forum myndreach 07/07/05 04:59 PM wet spot mold, brown mold, or just too moist? Contamination Forum myndreach 07/11/05 09:00 AM Re: wet spot mold, brown mold, or just too moist? Contamination Forum myndreach 07/11/05 09:44 AM Is this Jar okay? Please help. Contamination Forum myndreach 07/13/05 09:55 AM Re: Is this Jar okay? Please help. Contamination Forum myndreach 07/13/05 12:46 PM Re: Is this Jar okay? Please help. Contamination Forum myndreach 07/13/05 12:59 PM Re: discoloration Contamination Forum myndreach 07/14/05 09:25 AM Re: Are these mushrooms ruined? Contamination Forum myndreach 07/18/05 01:20 PM Is this jar okay? Please look and let me know. Contamination Forum myndreach 07/18/05 02:31 PM Re: Is this jar okay? Please look and let me know. Contamination Forum myndreach 07/18/05 05:17 PM Re: Is this jar okay? Please look and let me know. Contamination Forum myndreach 07/18/05 05:25 PM What is this? Are these salvagable? Contamination Forum myndreach 04/22/07 07:30 AM Re: what is this black stuff in my PF jar? Contamination Forum myndreach 04/22/07 07:42 AM Re: Weird Contamination Forum myndreach 04/22/07 07:44 AM Re: What is this? Are these salvagable? Contamination Forum myndreach 04/24/07 10:31 AM Re: hello all....first post here....got a question Contamination Forum myndreach 04/24/07 10:34 AM Myc piss, right? (pic) Contamination Forum myndreach 04/30/07 09:54 AM Re: Myc piss, right? (pic) Contamination Forum myndreach 04/30/07 10:42 AM Re: Cobweb mold? Contamination Forum myndreach 05/26/07 10:44 PM Pan cyan tray...what's going on? Contamination Forum myndreach 05/29/07 02:35 PM Re: Pan cyan tray...what's going on? Contamination Forum myndreach 05/29/07 08:01 PM
ButterWeasels
"ive inoculated many jars and ive only had 1 contam in las vegas but i didnt make the jar, it was from pjars, my very first grow. just flame sterilize often and youll have no problems in open air, in a clean house."
Re: Should I just case these cakes? Contamination Forum ButterWeasels 12/15/06 03:28 PM Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 07:42 AM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 07:51 AM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 08:46 AM Cobweb? Monotub Problems Contamination Forum ButterWeasels 10/18/07 09:25 AM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 10:46 AM Re: Cobweb? Monotub Problems Contamination Forum ButterWeasels 10/18/07 11:15 AM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 01:13 PM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 03:20 PM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/19/07 06:47 AM
I could go on to find a million of these!
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Dragonaut


Registered: 06/24/04
Posts: 6,487
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Re: The things people do for their grow that are a waste of time/money [Re: Cloneufc]
#11690646 - 12/21/09 05:24 AM (15 years, 30 days ago) |
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What the hell...edit that junk please. Open air inoculations FTW. Unless you are doing G2G or agar work, all you need are some alcohol wipes and a lighter/alc. lamp to keep the needle sterile.
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joska87
xenophobia



Registered: 09/29/09
Posts: 163
Loc: Idaho...No U Da Ho
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Re: The things people do for their grow that are a waste of time/money [Re: Cloneufc]
#11690649 - 12/21/09 05:25 AM (15 years, 30 days ago) |
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I could go on to find a million of these!
WHY
-------------------- When I was a boy i came across two nuns makin love in cave....That typa shit wont happen in the future...
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dancefloordale
Research Assistant


Registered: 02/13/09
Posts: 2,522
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Re: The things people do for their grow that are a waste of time/money [Re: Cloneufc]
#11690688 - 12/21/09 05:52 AM (15 years, 30 days ago) |
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Quote:
Cloneufc said: Drum Roll Please! Open air inoculation is the biggest waste of time. I put this together because its so damn funny.
"1 cubic foot of normal household air contains on average: 100,000 invisible particulates. So, every square inch of that air carries: One hell of a load of possible contaminate spores. Agar
Best success rate for open air inoculation. Is to continuously keep the needle covered. With an alc saturated gauze pad, foam, paper towel, or cotton balls. Otherwise, you will not BE LUCKY, FOR LONG." Agar
Anyone who recommends open air inoculations can be compared to a drunk who recommends drinking and driving. RR
Agar is a very experienced grower. His method you linked keeps the needle covered at all times in an alcohol soaked cloth. That's actually not 'open air'. Leave the needle in open air and see what happens. A glovebox is very easy to make. RR
dgls_jms
"id say a glovebox is a waste considering i never use one and rarely if ever get a contamination
I agree, I do all my work on a cookie sheet, door open to a hot oven. Is a little warm but hasent fail me yet."
"so I had a WBS jar that started showing contams a couple days after the myc started growing. I put it on my back porch and forgot about it. Now I'm not going to use but but the battle for life inside that jar has been amazing to watch. The myc pushed the mold to the very top, just a very few grains, and took over the whole jar. Fun to watch!"
"I ordered up 4 syringes from ralph. I also ordered a "sterile liquid culture" in a qt mason jar off of ebay. They ebay dude was taking forever to ship so I went out and bought some baby bottles, did my best to sterilize in the mic, knocked up after they cooled down. 30 hours later, mycelia is covering the bottom, so I swirled to distribute it throughout the culture. After I swirled, they became cloudy and remained that way, never saw the mycelia back at the bottom. This is when I realized I should invest in a pc, so I ordered one up, should arrive today. So while I'm waiting for my pc, my "sterile lc" arrived. It was clear and apeared to be contan free. knocked it up with a cc from a fresh spore syringe. 24 hours later, bottoms covered in mycelia. So last night, I swirled to disrtibute it. This morning the jar is all cloudy, and there is not as much mycelia on the bottom as last night. I am starting to think it is either ralphs syringes, or the "sterile aiport syringe" that this guy sent me. I figured when he said sterile it would still be in the autoclave bag, but it was a syringe stuffed with polyfill, no plunger, with the needle already attached, whole think rapped in foil, in a ziplock bag. I used ipa and flamed the needles. Also, It got really could here over the past week, went from 80's to around 70, dropping into the 50's at night. MY thermostat reads 70 inside, but I think it might get lower during the night, as the heater is not turned on yet. I know this would slow myclia growth, but would it speed up bacterial growth? I was thinking about setting the jar on a heating pad thing, but I don't want it to get too hot. Any help would be greatly appreciated. Thanks"
sHrOoMaCiDe
"On one of my recent attempts, i used 6 BRF/Verm jars and cubensis spore syringes. I inoculated in open air(dumb idea) all six jars about 3 weeks ago. The result: 4jars infected with green/black molds, and the other 2 surviving jars did not colonize or change at all. No growth. What happened? I inoculated 1-2 maybe even 3cc's in each jar. Still after 3 weeks, nothings happened. Any insights for me? Seems like failure. Really disappointing ya know?"
I got extremely lucky with innoculating my LC and fungi rye bag in open air. They are both progressing without contamination. However, the bag is progressing at a glacial speed. The LC is almost done colonizing. When its done should I suck up some of that mycelium and inject it into my fungi bag to speed it up?
I've never had success in growing mushrooms yet, but my 3rd attempt was the closest. I inoculated two quart sized pre-sterilized rye grain jars back in july. The mycelium grew in one of them but not the other. However, it grew about halfway up the jar and then stopped. It went dormant or something. so my question is, can i re-inoculate the jar that didn't grow any mycelium? its really old, but theres no contamination.
VampireSlayer Why i'm done with OPEN AIR INOCULATIONS
"Just wanted to say...i did open air innoc for a year with pretty good success. actually only till the last couple months have I been getting contams...I do not fuck with open air period!! it is just a 50/50 in my oppinion. I put the blame on a vendor here about a contammed syringe but NOW i'm not sure if it was the the vendor to blame or if it was me...the point is if you use any other method (flow air or still air) then you can easssily put the blame on the culture or syringe (talking cubie syringer growers here not graintograin and stuff) but grain to grain I WOULD NEVER do in open air but NOW i'll never even do open air period...it's too risky period"
NinjaGaiden
"I've had 100% success with using the oven tek. I do everything: Make syringes, inoculate jars, G2G transfers using the oven.
I'm not at all trying to debate if the tek itself is bogus or not, but it does work for some." NinjaGaiden
"more like 100% LUCK
and I will debate it...
the Oven tek is crap!~" Roadkill
jeetered
"i always open air inoculate. I like "glacial" that's great, i've found that with spore to grain before too. LC to grain is much much faster. I don't know anything about adding LC to it to speed it up though."
"i do pretty much everything open air except agar work."
Re: What in the world! Contamination Forum jeetered 03/25/07 06:58 PM Re: Contamination??? Contamination Forum jeetered 05/17/07 08:43 PM Re: Contamination??? Contamination Forum jeetered 05/18/07 07:51 PM Re: Bacillus contam in rye Contamination Forum jeetered 07/01/07 09:47 AM black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/07/07 08:08 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/07/07 08:34 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/07/07 10:44 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/07/07 10:51 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/08/07 05:37 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/09/07 05:31 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum jeetered 11/10/07 07:54 AM Re: black,blue,green,and purple molds in 3 jars, 18 total contam'd (failed g2g ) Contamination Forum
figgusfiddus
"Yeah, open air inoculation isn't really that risky. When I get screwed up, it's usually the substrate conditions that do it."
Re: Contamination? Contamination Forum figgusfiddus 05/10/07 01:15 PM Re: Contamination? Contamination Forum figgusfiddus 05/14/07 10:39 AM Re: Daaaaaaaamn! Green Mold... Contamination Forum figgusfiddus 06/13/07 06:53 PM Re: weird growth on cake (pic) Contamination Forum figgusfiddus 06/16/07 05:05 PM Re: weird growth on cake (pic) Contamination Forum figgusfiddus 06/16/07 08:28 PM Re: What kind of infection is this? [pics] Contamination Forum figgusfiddus 06/16/07 08:50 PM Re: What kind of infection is this? [pics] Contamination Forum figgusfiddus 06/16/07 08:57 PM Re: Daaaaaaaamn! Green Mold... Contamination Forum figgusfiddus 06/17/07 02:19 PM Re: weird growth on cake (pic)update Contamination Forum figgusfiddus 06/17/07 06:13 PM Re: wet looking caps Contamination Forum figgusfiddus 06/17/07 06:15 PM Re: weird growth on cake (pic) Contamination Forum figgusfiddus 06/18/07 02:49 PM Re: ...is this Trich? Contamination Forum figgusfiddus 07/06/07 01:27 PM Re: ...is this Trich? Contamination Forum figgusfiddus 07/06/07 01:28 PM Low humidity promoting molds (esp. trichoderma)? Contamination Forum figgusfiddus 07/28/07 04:13 PM Re: Low humidity promoting molds (esp. trichoderma)? Contamination Forum figgusfiddus 07/30/07 10:27 PM Re: If i have a piece of bread with green mold on it... Contamination Forum figgusfiddus 01/30/08 03:46 PM
myndreach
"do most people here actually use a glovebox or something? SWIM has never had contams as a result of air...the one they got was because of a bad syringe.
lysol for surfaces, oust for the air, dust mask and rubber gloves."
Re: Worst Contam Pic Contamination Forum myndreach 05/27/05 08:55 AM Re: tiny black spec on mycelium Contamination Forum myndreach 06/01/05 04:26 PM First grow...AFOAF is freaking out...come this far....contam? Contamination Forum myndreach 06/10/05 09:27 PM Re: First grow...AFOAF is freaking out...come this far....contam? Contamination Forum myndreach 06/10/05 09:50 PM Re: black stuff in syringe Contamination Forum myndreach 06/11/05 10:48 PM Re: Possible contam on mushrooms? Contamination Forum myndreach 06/12/05 08:22 PM Re: brown mycellium? Contamination Forum myndreach 06/12/05 08:23 PM Re: spots on white mycelium Contamination Forum myndreach 06/13/05 09:00 AM Contam or Myc Piss? Contamination Forum myndreach 06/13/05 11:06 AM Re: Contam or Myc Piss? Contamination Forum myndreach 06/14/05 07:27 AM Contams in jar? Brown/Orange/Wet spot mold? Need help quick please. Contamination Forum myndreach 07/02/05 09:51 AM Re: Question about appearance of some recently acquired dried shrooms Contamination Forum myndreach 07/07/05 04:59 PM wet spot mold, brown mold, or just too moist? Contamination Forum myndreach 07/11/05 09:00 AM Re: wet spot mold, brown mold, or just too moist? Contamination Forum myndreach 07/11/05 09:44 AM Is this Jar okay? Please help. Contamination Forum myndreach 07/13/05 09:55 AM Re: Is this Jar okay? Please help. Contamination Forum myndreach 07/13/05 12:46 PM Re: Is this Jar okay? Please help. Contamination Forum myndreach 07/13/05 12:59 PM Re: discoloration Contamination Forum myndreach 07/14/05 09:25 AM Re: Are these mushrooms ruined? Contamination Forum myndreach 07/18/05 01:20 PM Is this jar okay? Please look and let me know. Contamination Forum myndreach 07/18/05 02:31 PM Re: Is this jar okay? Please look and let me know. Contamination Forum myndreach 07/18/05 05:17 PM Re: Is this jar okay? Please look and let me know. Contamination Forum myndreach 07/18/05 05:25 PM What is this? Are these salvagable? Contamination Forum myndreach 04/22/07 07:30 AM Re: what is this black stuff in my PF jar? Contamination Forum myndreach 04/22/07 07:42 AM Re: Weird Contamination Forum myndreach 04/22/07 07:44 AM Re: What is this? Are these salvagable? Contamination Forum myndreach 04/24/07 10:31 AM Re: hello all....first post here....got a question Contamination Forum myndreach 04/24/07 10:34 AM Myc piss, right? (pic) Contamination Forum myndreach 04/30/07 09:54 AM Re: Myc piss, right? (pic) Contamination Forum myndreach 04/30/07 10:42 AM Re: Cobweb mold? Contamination Forum myndreach 05/26/07 10:44 PM Pan cyan tray...what's going on? Contamination Forum myndreach 05/29/07 02:35 PM Re: Pan cyan tray...what's going on? Contamination Forum myndreach 05/29/07 08:01 PM
ButterWeasels
"ive inoculated many jars and ive only had 1 contam in las vegas but i didnt make the jar, it was from pjars, my very first grow. just flame sterilize often and youll have no problems in open air, in a clean house."
Re: Should I just case these cakes? Contamination Forum ButterWeasels 12/15/06 03:28 PM Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 07:42 AM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 07:51 AM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 08:46 AM Cobweb? Monotub Problems Contamination Forum ButterWeasels 10/18/07 09:25 AM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 10:46 AM Re: Cobweb? Monotub Problems Contamination Forum ButterWeasels 10/18/07 11:15 AM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 01:13 PM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/18/07 03:20 PM Re: Cobweb? Problems Contamination Forum ButterWeasels 10/19/07 06:47 AM
I could go on to find a million of these!
That was fucking hilarious. Thank you.
-------------------- Everything posted by the user dancefloordale, aside from what is written in this here clause is completely and utterly fictitious, despite any information read (or seen), above (or below) that might lead you to believe otherwise.
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Cloneufc
Master Exploder!



Registered: 11/15/07
Posts: 1,237
Loc: Las Vegas
Last seen: 1 year, 5 months
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Re: The things people do for their grow that are a waste of time/money [Re: dancefloordale]
#11690691 - 12/21/09 05:54 AM (15 years, 30 days ago) |
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Thank you! Finally someone gets it! lol
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dgls_jms
festival follower

Registered: 03/24/09
Posts: 297
Last seen: 12 years, 7 months
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Re: The things people do for their grow that are a waste of time/money [Re: Cloneufc]
#11690698 - 12/21/09 06:02 AM (15 years, 30 days ago) |
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Quote:
"I ordered up 4 syringes from ralph. I also ordered a "sterile liquid culture" in a qt mason jar off of ebay. They ebay dude was taking forever to ship so I went out and bought some baby bottles, did my best to sterilize in the mic, knocked up after they cooled down. 30 hours later, mycelia is covering the bottom, so I swirled to distribute it throughout the culture. After I swirled, they became cloudy and remained that way, never saw the mycelia back at the bottom. This is when I realized I should invest in a pc, so I ordered one up, should arrive today. So while I'm waiting for my pc, my "sterile lc" arrived. It was clear and apeared to be contan free. knocked it up with a cc from a fresh spore syringe. 24 hours later, bottoms covered in mycelia. So last night, I swirled to disrtibute it. This morning the jar is all cloudy, and there is not as much mycelia on the bottom as last night. I am starting to think it is either ralphs syringes, or the "sterile aiport syringe" that this guy sent me. I figured when he said sterile it would still be in the autoclave bag, but it was a syringe stuffed with polyfill, no plunger, with the needle already attached, whole think rapped in foil, in a ziplock bag. I used ipa and flamed the needles. Also, It got really could here over the past week, went from 80's to around 70, dropping into the 50's at night. MY thermostat reads 70 inside, but I think it might get lower during the night, as the heater is not turned on yet. I know this would slow myclia growth, but would it speed up bacterial growth? I was thinking about setting the jar on a heating pad thing, but I don't want it to get too hot. Any help would be greatly appreciated. Thanks"
This wasn't my thread.
-------------------- Not that you lied to me, but that I no longer believe you has shaken me.
"I have done that" says my memory. "I cannot have done that" says my pride, and remains inexorable. Eventually - memory yields.
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veda_sticks
Cultivator




Registered: 07/29/07
Posts: 14,191
Loc: UK
Last seen: 5 years, 14 days
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Re: The things people do for their grow that are a waste of time/money [Re: Cloneufc]
#11690702 - 12/21/09 06:07 AM (15 years, 30 days ago) |
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Im a little disapointed to find people still saying gloveboxes are a waste of time.
I dont doubt that a SELECT group of people are very successfull with doing inoculations in open air, however from being on this forum nearly every day for the past 3-4 years and from my own personal experiences. Not using a glovebox has been the cause of alot of failures.
The needle is not the only point that contamination could get into a jar, the jar itself is also at risk. Spores landing on the jar lid, on the inoc holes and getting pushed through during inoculation.
I tried and tried using open air inoculations, keeping the needle covered, wiping the jar lid with IPA, even flaming and inoculating wile the needle was still hot and i would get 90% contam rate.
Glovebox's are certainly not a waste of time and considering how cheaply 1 can be constructed and if made right, they are very easy to work inside.
If you use a glovebox you also save money on all those chemicals you are spraying everywhere. No need to empty a bottle of lysol and a can of oust, just a nice few spraws around your glovebox.
IMO open air inoculations are a waste of time, too much effort spent trying to sterilise the air and surfaces. Yoiu'd be better of spending a few minutes each day keeping your place tidy and clean.
I made mine oiut of cardboard.

Total cost was for that was somewhere around £1 after buying clinging film.
99% success rate, inoculating substrates, LC making prints and syringes.
My flat however does have a high spore count. My bedroom (which i dont use) grows green mold. Its winter just now, i put a chair in there which i dont use and its growing mold after just a month and its bloody cold in there, muyst be close to 0.
Not everyone has a very clean place thats dry with a low spore count.
Also, i noticed someone put down lighting as a waste. The best mushrooms i have grown, and that i have seen grown across the forum has been with bright light in the daylight range on a day night schedule.
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tugwax
Anomaly



Registered: 11/12/09
Posts: 160
Loc: Little Britain
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Re: The things people do for their grow that are a waste of time/money [Re: veda_sticks]
#11691531 - 12/21/09 11:12 AM (15 years, 30 days ago) |
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The thread is still controversial but seems to have taken a turn for the better.
Is it just me or is everyone calling a still air box a glovebox? If it was a glovebox then wouldn't it have gloves attached?
-------------------- Freedom is as great as one's tolerance for ambiguity.
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nexus1946
Rebel



Registered: 06/25/09
Posts: 2,315
Loc: In the moment
Last seen: 8 years, 6 months
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Re: The things people do for their grow that are a waste of time/money [Re: tugwax]
#11691673 - 12/21/09 11:30 AM (15 years, 30 days ago) |
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Still Air/Glove Box
  
The only thing better would be a Laminar Flow Hood. (Soon to come)
-------------------- Gypsum/Drywall Tek
The Story Of Russell The Texas (Cube) Bear

The human race's prospects of survival were considerably better when we were defenceless against tigers than they are today when we have become defenceless against ourselves.
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jdolphinbabe
One Who Seeks 2 Know



Registered: 09/26/08
Posts: 402
Loc: Washington
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Re: The things people do for their grow that are a waste of time/money [Re: tugwax]
#11691725 - 12/21/09 11:39 AM (15 years, 30 days ago) |
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this thread is a waste.... first things i did make a glove box out of plastic so it can be bleached clean and 3 months later bought a pc! at the very least it has been entertaining......as far as threads go..... i am sure this one will die and get resurfaced so many times....
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tugwax
Anomaly



Registered: 11/12/09
Posts: 160
Loc: Little Britain
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Re: The things people do for their grow that are a waste of time/money [Re: jdolphinbabe]
#11691810 - 12/21/09 11:53 AM (15 years, 30 days ago) |
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Quote:
nexus1946 said: Still Air/Glove Box
  
The only thing better would be a Laminar Flow Hood. (Soon to come) 
Yes I made one just like that when waiting for the spores for my first grow. Contam free but just too awkward to use. Flow hood construction commenced immediately and has been in use ever since.
If you don't have any problem using that glovebox then I'd recommend a filtered hole on the side of the box to equalize the pressure differences when you move your hands in and out.
I'm sure this kind of design is much better than a still air box for just a little more work.
Quote:
jdolphinbabe said: this thread is a waste.... first things i did make a glove box out of plastic so it can be bleached clean and 3 months later bought a pc! at the very least it has been entertaining......as far as threads go..... i am sure this one will die and get resurfaced so many times....
You now use a PC instead of a glovebox?
-------------------- Freedom is as great as one's tolerance for ambiguity.
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Edited by tugwax (12/21/09 12:24 PM)
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