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Offlinewocka
Lurker


Registered: 10/26/07
Posts: 771
Last seen: 7 years, 3 months
Re: How do you put the needle on the syringe??? [Re: MycoUnderground]
    #11693887 - 12/21/09 05:34 PM (15 years, 30 days ago)

take a clean shot glass and put that in a glovebox with ur sterile water syringe n spore print.

shoot water into shot glass

use a paper clip to scrape spores off into water

suck up half of the water from the bottom and the spores will stay suspended on the top.

then suck up the top layer of water which will pull in the spores.. then suck up excess water and shake.. perfect syringe

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Offlinebirdbrain84
we are living in the future
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Registered: 04/26/09
Posts: 293
Loc: nv
Last seen: 12 years, 3 months
Re: How do you put the needle on the syringe??? [Re: wocka]
    #11694931 - 12/21/09 08:23 PM (15 years, 29 days ago)

Yes I know I'm worrying a lot about little things but So far I've screwed up 7 probably 8 jars.  Hopefully this one isn't contaminated but so far after 3 days there is no growth at all.

Yeah I know some people can get away with being sloppy and not contaminate their jars but I've been so careful and still can't do it right thats why im so worried about all these tiny details..

I have 4 other jars that I havent inoculated yet so if this one doesnt work out I can try again.  But if its the syringe thats contaminated those wont work either

I know Ill probably end up doing it eventually but wont I have much more chance of contamination if I make my own syringe/


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I don't suffer from insanity, i enjoy every minute of it.

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OfflineGrumpyorc
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Registered: 09/14/09
Posts: 2,041
Last seen: 5 years, 3 months
Re: How do you put the needle on the syringe??? [Re: birdbrain84]
    #11695890 - 12/21/09 10:46 PM (15 years, 29 days ago)

if you contaminate your syringe while making the print you get like 4 other chances for the price of one syringe. 

and im a heathenly slob who does all his work in the kitchen and i aint never had a problem.  just use a glovebox. if you dont have one turn a clear tub upside down on the table push it out over the ledge, spray the inside with lysol or water or whatever the heck your gona use and give it a few min to let it settle. reach in from underneath
its not the best but its worked fine for me.

at the very least order a new syringe immediately. if your grow fails and you cant pinpoint the source then you need to start COMPLETELY over to make sure you get rid of the guilty culprit


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Offlinebirdbrain84
we are living in the future
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Registered: 04/26/09
Posts: 293
Loc: nv
Last seen: 12 years, 3 months
Re: How do you put the needle on the syringe??? [Re: Grumpyorc]
    #11696290 - 12/21/09 11:45 PM (15 years, 29 days ago)

Quote:

Grumpyorc said:
if you contaminate your syringe while making the print you get like 4 other chances for the price of one syringe. 

and im a heathenly slob who does all his work in the kitchen and i aint never had a problem.  just use a glovebox. if you dont have one turn a clear tub upside down on the table push it out over the ledge, spray the inside with lysol or water or whatever the heck your gona use and give it a few min to let it settle. reach in from underneath
its not the best but its worked fine for me.

at the very least order a new syringe immediately. if your grow fails and you cant pinpoint the source then you need to start COMPLETELY over to make sure you get rid of the guilty culprit



But if I make my own syringe and contaminate it wouldnt all the syringes I make be contaminated?

The first time I used a clear tub turned upside down it didnt work now I use the same clear tub with seran wrap on the open end

I would order a new syringe but I dont want to keep spending my money on more syringes if its not going to work


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I don't suffer from insanity, i enjoy every minute of it.

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OfflineGlassApex
Brock Samson


Registered: 07/26/09
Posts: 224
Last seen: 10 years, 9 months
Re: How do you put the needle on the syringe??? [Re: MycoUnderground]
    #11696583 - 12/22/09 12:43 AM (15 years, 29 days ago)

I'm sure there's a tek around here somewhere, but me typing it out will probably be faster than me finding it...

I'll start with empty syringes;
-Boil water
-Suck up boiling water with your empty syringes a couple times and empty them out each time in the sink. Don't burn yourself.
-On the third time, leave the boiling water in the syringes.
-Store them somewhere over night.

With your sterilized water filled syringes;
-Take your ziplock bag with your spore print in it
-Make a hole with one of your syringes
-Inject all of the water from multiple syringes in to the bag.
-Once you have enough water in the bag, scratch the spores off the aluminum foil from the outside.
-Then shake it until it's mixed up really well.
-Take your empty syringes, and one by one re-enter the same hole and suck up your desired amount of water.


Obviously, you'll need to take the proper procedures to sterilize the needle each time. Be careful not to stab through the bag. The amount of syringes needed depends on the size of the print. For a 2" spore print, I like to use 5 syringes. It's pretty easy. The only way it can get contaminated is through the little hole you created with the needle. Sure beats doing it in a shot glass.. :thumbup:

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Offlinebirdbrain84
we are living in the future
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Registered: 04/26/09
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Last seen: 12 years, 3 months
Re: How do you put the needle on the syringe??? [Re: GlassApex]
    #11698255 - 12/22/09 11:20 AM (15 years, 29 days ago)

Thanks do I need to sterilze the bag somehow first?


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I don't suffer from insanity, i enjoy every minute of it.

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OfflineGlassApex
Brock Samson


Registered: 07/26/09
Posts: 224
Last seen: 10 years, 9 months
Re: How do you put the needle on the syringe??? [Re: birdbrain84]
    #11699843 - 12/22/09 03:14 PM (15 years, 29 days ago)

Well, you should've sterilized the bag before you put the spore print in.

I always wipe them out lightly with an alcohol rag, then leave just a very small gap in the ziplock opening to dry out. I usually wait about 20 minutes to be safe before putting my print in, then close it up.

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Offlinebirdbrain84
we are living in the future
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Registered: 04/26/09
Posts: 293
Loc: nv
Last seen: 12 years, 3 months
Re: How do you put the needle on the syringe??? [Re: GlassApex]
    #11702691 - 12/22/09 09:21 PM (15 years, 28 days ago)

ok thanks


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I don't suffer from insanity, i enjoy every minute of it.

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