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fundamentalchair
Stand up



Registered: 07/13/09
Posts: 801
Last seen: 13 years, 7 months
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Is this normal? Phreaky LC. Manspooge? Zombie Pandemic?
#11672102 - 12/17/09 10:12 PM (15 years, 1 month ago) |
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LME LC:
This looks either mega good or mega bad. I haven't decided which.

Edit: title
-------------------- semper necessitas probandi incumbit ei qui agit
Sclerotia FAQ... If ya ain't got any stones, grow some.
Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually),  me.
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Edited by fundamentalchair (12/17/09 11:50 PM)
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pblcenmy1
str8 noob



Registered: 11/24/09
Posts: 221
Loc: Colo. Rockies
Last seen: 8 years, 11 days
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why so yellow? or, why so brown.
Did you use honey or dextrose or sucrose?... if so how much?
are those glass shards in there too?
-------------------- ~LoveLife~
Down 2 Earth, Up N Space!
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fundamentalchair
Stand up



Registered: 07/13/09
Posts: 801
Last seen: 13 years, 7 months
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Quote:
pblcenmy1 said: why so yellow? or, why so brown.
Did you use honey or dextrose or sucrose?... if so how much?
are those glass shards in there too?
LME = Light Malt Extract 1g / 100ml I have a glass bead in there somewhere to theoretically break up the myc when shaken.
I think a jellyfish is growing...
-------------------- semper necessitas probandi incumbit ei qui agit
Sclerotia FAQ... If ya ain't got any stones, grow some.
Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually),  me.
My Trade list.
Ghetto Tek: Auto FAE & Light
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Damion5050
Mush Doctor


Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 11 months
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Doesn't look like any LC I have ever seen..
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shroom239
aka K.T.




Registered: 08/12/09
Posts: 550
Last seen: 9 years, 9 days
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Good god. Mine looked nothing like that.
-------------------- Disclaimer: Any picture or statement I post here is from research I do on my free time, I do not actually partake in this hobby.
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fundamentalchair
Stand up



Registered: 07/13/09
Posts: 801
Last seen: 13 years, 7 months
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Quote:
Damion5050 said: Doesn't look like any LC I have ever seen..

Quote:
shroom239 said: Good god. Mine looked nothing like that.
Is it junk?
-------------------- semper necessitas probandi incumbit ei qui agit
Sclerotia FAQ... If ya ain't got any stones, grow some.
Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually),  me.
My Trade list.
Ghetto Tek: Auto FAE & Light
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Damion5050
Mush Doctor


Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 11 months
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Hard to say until u test it out.. That is why sugar based LC are difficult and sometimes a pain in the ass.
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shroom239
aka K.T.




Registered: 08/12/09
Posts: 550
Last seen: 9 years, 9 days
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I honestly do not know. I just did my first LC and it was, well, not as plentiful as yours. If that is good LC you did a bang up job for sure.
-------------------- Disclaimer: Any picture or statement I post here is from research I do on my free time, I do not actually partake in this hobby.
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pblcenmy1
str8 noob



Registered: 11/24/09
Posts: 221
Loc: Colo. Rockies
Last seen: 8 years, 11 days
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well the snotty substance i believe to be the mycelium network. Extra ordinary of course. I guess knock up some jars and go from there.
-------------------- ~LoveLife~
Down 2 Earth, Up N Space!
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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This is why I like grain LC's
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Damion5050
Mush Doctor


Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 11 months
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Re: Is this normal? [Re: Shea25]
#11672243 - 12/17/09 10:41 PM (15 years, 1 month ago) |
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Quote:
Shea25 said: This is why I like grain LC's
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noobieshroomie
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Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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also
-noobie-
-------------------- AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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fundamentalchair
Stand up



Registered: 07/13/09
Posts: 801
Last seen: 13 years, 7 months
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Quote:
Damion5050 said:
Quote:
Shea25 said: This is why I like grain LC's


Quote:
I honestly do not know. I just did my first LC and it was, well, not as plentiful as yours. If that is good LC you did a bang up job for sure.
Bang up job? All i did was shoot some spores into some sterile LME water.
I had some other cultures where the mycelium was literally growing up the side of the jar. Absolutely bizzare. I'll see if I have one that I didn't shake it off into the LC.
-------------------- semper necessitas probandi incumbit ei qui agit
Sclerotia FAQ... If ya ain't got any stones, grow some.
Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually),  me.
My Trade list.
Ghetto Tek: Auto FAE & Light
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shroom239
aka K.T.




Registered: 08/12/09
Posts: 550
Last seen: 9 years, 9 days
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I used honey and a spore print for my LC. I got a good bit but nothing like that, maybe the honey had something to do with it. Still trying to perfect my method for LC.
-------------------- Disclaimer: Any picture or statement I post here is from research I do on my free time, I do not actually partake in this hobby.
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Damion5050
Mush Doctor


Registered: 05/01/08
Posts: 12,493
Loc: Lost In Translation !
Last seen: 3 years, 11 months
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Quote:
shroom239 said: I used honey and a spore print for my LC. I got a good bit but nothing like that, maybe the honey had something to do with it. Still trying to perfect my method for LC.
Be careful using a print.. Cause almost every print I get is dirty and will cause ur LC to go bad.. that is why i always go print to agar then to grain LC
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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if you do use prints use very very little
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shroom239
aka K.T.




Registered: 08/12/09
Posts: 550
Last seen: 9 years, 9 days
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Quote:
Damion5050 said:
Be careful using a print.. Cause almost every print I get is dirty and will cause ur LC to go bad.. that is why i always go print to agar then to grain LC
Yeah I was thinking that as I was doing it but everything turned out alright. I need to get some petri dishes and agar soon, cant pinpoint the hold up on that but its there.
Quote:
Shea25 said: if you do use prints use very very little
Oh I did.
-------------------- Disclaimer: Any picture or statement I post here is from research I do on my free time, I do not actually partake in this hobby.
Edited by shroom239 (12/17/09 10:58 PM)
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fundamentalchair
Stand up



Registered: 07/13/09
Posts: 801
Last seen: 13 years, 7 months
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This one shows the myc on the glass as well as this mycelial layer on top of the LC. This shit is whack.
I SOOOOOOO hope this stuff is legit, and not the zombie causing pandemic. Actually, that would be pretty cool too.
-------------------- semper necessitas probandi incumbit ei qui agit
Sclerotia FAQ... If ya ain't got any stones, grow some.
Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually),  me.
My Trade list.
Ghetto Tek: Auto FAE & Light
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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that does not look good
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Pscientist
KushKaptain




Registered: 11/13/09
Posts: 2,692
Loc: Sirius X1
Last seen: 22 days, 13 hours
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Eviiiillll......Eeeeeeeviiiiiiillll!
That shit is evil man, Id get rid of it, I toss lc's without a second thought, specially if theyre gnarly looking, but thats just sick...
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fundamentalchair
Stand up



Registered: 07/13/09
Posts: 801
Last seen: 13 years, 7 months
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Quote:
Pscientist said: Eviiiillll......Eeeeeeeviiiiiiillll!
That shit is evil man, Id get rid of it, I toss lc's without a second thought, specially if theyre gnarly looking, but thats just sick...
Both?
The second one is from a LC I got from sporeworks. I let 3 other jars of LC that I didn't noc up sit around to see if they contamed, none did. Did a GB noc, alcohol sponge over noc point. All LC is using self healing injection ports and double tyvek (one on top, one on bottom) for the breather hole.
I'm just completely confused.
-------------------- semper necessitas probandi incumbit ei qui agit
Sclerotia FAQ... If ya ain't got any stones, grow some.
Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually),  me.
My Trade list.
Ghetto Tek: Auto FAE & Light
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Shea25
Just some guy



Registered: 01/27/09
Posts: 7,772
Loc: Westcoast Canada
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hmm weird. Personally after that needle has been flamed red hot it should only come into contact with one thing after, and thats the self healing port
Edited by Shea25 (12/17/09 11:14 PM)
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noobieshroomie
Back again




Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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Re: Is this normal? [Re: Shea25]
#11672439 - 12/17/09 11:15 PM (15 years, 1 month ago) |
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test a jar and see that will tell you if its bad or not
-noobie-
-------------------- AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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fundamentalchair
Stand up



Registered: 07/13/09
Posts: 801
Last seen: 13 years, 7 months
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Re: Is this normal? [Re: Shea25]
#11672467 - 12/17/09 11:23 PM (15 years, 1 month ago) |
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Quote:
hmm weird. Personally after that needle has been flamed red hot it should only come into contact with one thing after, and thats the self healing port
I loaded up the GB w/ the syringe, sterile (unopened) needle, LC (thoroughly wiped down with IPA), sponge sitting in container with 70% IPA (soak 24+ hr). Spray the hell out of the GB with 70% IPA. Seal the arm holes with zip-lok bags glued to hang over the holes, IPA between tub and bags to form an air tight seal. Wait 5 mins before work.
Glove up, 70% IPA hand sanitizer up to the elbows. arms in, wait a few seconds for the air to settle. Grab syringe with left hand (plunger up), needle with right hand (point up). Break the seal at the bezel end, twist off syringe cap with index/middle finger, flip hand over, install needle (pop to lock takes about 1.5s). remove sponge, place on injector port. remove needle scabbard, inject through sponge. Withdraw holding sponge on port, recover with scabbard. Take syringe out, remove scabbard, fill with IPA. Flame needle, dump out scabbard and recover.
Is that a bad process?
Quote:
test a jar and see that will tell you if its bad or not
-noobie-
I'm on it. Noced up some jars 2 days ago, waiting to see some movement.
-------------------- semper necessitas probandi incumbit ei qui agit
Sclerotia FAQ... If ya ain't got any stones, grow some.
Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually),  me.
My Trade list.
Ghetto Tek: Auto FAE & Light
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noobieshroomie
Back again




Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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looks good but are you filling your needle cover with alcohol then putting it back on the syringe?
and are you using those wild spores or ones you bought?
-noobie-
-------------------- AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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fundamentalchair
Stand up



Registered: 07/13/09
Posts: 801
Last seen: 13 years, 7 months
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Quote:
noobieshroomie said: looks good but are you filling your needle cover with alcohol then putting it back on the syringe?
and are you using those wild spores or ones you bought?
-noobie-
Fill the cover while I flame the needle, pour it (mostly) out as I'm taking the needle off the flame and immediately recovering.
All spores/cultures I have worked with have come from shroomery vendors.
While I do have a few wild prints, I won't mess with those till I can get into agar work.
-------------------- semper necessitas probandi incumbit ei qui agit
Sclerotia FAQ... If ya ain't got any stones, grow some.
Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually),  me.
My Trade list.
Ghetto Tek: Auto FAE & Light
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TacoHerder
Bluedavenger



Registered: 06/10/09
Posts: 10,107
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Quote:
fundamentalchair said: LME LC:
This looks either mega good or mega bad. I haven't decided which.

ok, ok... tell us the truth... you jizzed in the jar didnt you
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noobieshroomie
Back again




Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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theres no need to fill it with alcohol that can actually damage the spores
but i have no clue as to why your LC looks like that as for those prints my offer still stands check out my edible trade list in my journal im always adding new stuff
id love to be able to add those as well and give you clean cultures too
-noobie-
-------------------- AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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fundamentalchair
Stand up



Registered: 07/13/09
Posts: 801
Last seen: 13 years, 7 months
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Quote:
ok, ok... tell us the truth... you jizzed in the jar didnt you 
Dude, if I could man spooge like that don't you think I could find something better to do than post shit up here? You know, like make a bukkakke flik?
The back lighting definitely makes it look more defined, but with just the flash or front lighting it doesn't look like it does in person.
Quote:
as for those prints my offer still stands
They'll be out in the mail with like the other 3-4 packages that have to go out. I was just not trying to announce anything unless you wanted to keep it on the DL.
-------------------- semper necessitas probandi incumbit ei qui agit
Sclerotia FAQ... If ya ain't got any stones, grow some.
Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually),  me.
My Trade list.
Ghetto Tek: Auto FAE & Light
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noobieshroomie
Back again




Registered: 07/18/08
Posts: 12,769
Loc: Not Too Sure
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 -noobie-
-------------------- AMU
Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.
Citric said:
Your signature is wrong on colonization temps!
GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT
ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef
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fungus_tao
Hah Zah!



Registered: 05/10/06
Posts: 1,856
Loc:
Last seen: 7 years, 3 months
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I did an LC with a particularly vigorous strain of h. ulmarius. After about a week I had a 1/4" layer of myc on top. I could turn the jar upside down and the the myc was so strong it actually created a seal, and held the water in place! Anyways, the point is it is a very valid theory you have a vigorous strain. Was this multi-spore or did you isolate? I would keep at it, because you might be onto something good. Good luck, good growing.
-------------------- Follow the light
The Light is your guide.
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fundamentalchair
Stand up



Registered: 07/13/09
Posts: 801
Last seen: 13 years, 7 months
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Quote:
Was this multi-spore or did you isolate?
first one is from MS, second is from LC via Sporeworks. They sell some mean Blue Oyster, apparently.
Funny thing is, I have some karo LC that was made from the same print as the first pic's genetic donor (same print, made 2 syringes). The karo LC has some little itsy balls of myc floating at the bottom. It's quite sad, really.
I swear these LME LC's have been growing in the fridge...
-------------------- semper necessitas probandi incumbit ei qui agit
Sclerotia FAQ... If ya ain't got any stones, grow some.
Will work for Laetiporus sulphureus culture/spores (or any other Laetiporus actually),  me.
My Trade list.
Ghetto Tek: Auto FAE & Light
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dcox420
Adrenaline Junkie



Registered: 08/06/08
Posts: 157
Loc: South East USA
Last seen: 11 years, 2 months
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HOLY SHIT MAN....seriously thats the stuff horror films are made of. Chunk that shit.
--------------------
My Busa...Nothing better than hitting the highway at 3am with no traffic and kicking it up to 110-120.
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