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Offlinersinatra13
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Germinating Spores
    #11669200 - 12/17/09 03:22 PM (15 years, 1 month ago)

lately, i've been trying to make my own syringes from prints, and then inoculate a batch of cakes, as opposed to buying more syringes, because i need to learn to be self sufficient, however, i havent been having much luck.

its not sterile procedure that i lack, i havent ever gotten any contams, during colonization.

anyways, this is what almost always happens. i make the syringe the usual way (sterilized shot glass, scrape spores in, shoot about 4-6 cc's of sterile water, swirl, suck back up) after this however, i never see any spore clumps in my syringes, but i mean i know they must be in there. so i inoculate a few jars, wait weeks, and weeks, and nothing happens. no contam... but no germination either.

can anybody tell me what i may be doing wrong when making my syringes?


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Invisibleprismism
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Registered: 05/11/09
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Re: Germinating Spores [Re: rsinatra13]
    #11669227 - 12/17/09 03:25 PM (15 years, 1 month ago)

keep trying.
everything sounds good.


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Offlinersinatra13
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Re: Germinating Spores [Re: prismism]
    #11669248 - 12/17/09 03:28 PM (15 years, 1 month ago)

Quote:

prismism said:
keep trying.
everything sounds good.




thanks for your input prismism:thumbup:


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Invisiblenoobieshroomie
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Re: Germinating Spores [Re: prismism]
    #11669250 - 12/17/09 03:28 PM (15 years, 1 month ago)

do you see clumps of spores in the shot glass?

    -noobie-


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AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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Offlinesansa

Registered: 11/17/09
Posts: 647
Last seen: 10 years, 4 months
Re: Germinating Spores [Re: noobieshroomie]
    #11669265 - 12/17/09 03:31 PM (15 years, 1 month ago)

If you're using a needle tip to swirl them you may be damaging the spores. Try using an inoculation loop-shaped piece of wire.

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Offlinersinatra13
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Re: Germinating Spores [Re: noobieshroomie]
    #11669307 - 12/17/09 03:35 PM (15 years, 1 month ago)

Quote:

noobieshroomie said:
do you see clumps of spores in the shot glass?

    -noobie-




yes however i wasnt able to really suck them up well.
after i suck up the spore water, there are usually still some clumps left in the shot glass


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Invisiblemyco_mikey_mo
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Re: Germinating Spores [Re: rsinatra13]
    #11669331 - 12/17/09 03:38 PM (15 years, 1 month ago)

and you dont always have to see spores in the syringe for them to really be there. they are microscopic


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Offlinersinatra13
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Re: Germinating Spores [Re: myco_mikey_mo]
    #11669399 - 12/17/09 03:48 PM (15 years, 1 month ago)

Quote:

myco_mikey_mo said:
and you dont always have to see spores in the syringe for them to really be there. they are microscopic




i know, i said that in my post, i said that i cant see them but i know theyre there. but still they dont germinate

and before anyone asks, yes i allow them a 24 hour hydrating period


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Invisiblepsykiidellic
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Re: Germinating Spores [Re: rsinatra13]
    #11669444 - 12/17/09 03:55 PM (15 years, 1 month ago)

could be a dead print


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Offlinersinatra13
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Re: Germinating Spores [Re: psykiidellic]
    #11669625 - 12/17/09 04:18 PM (15 years, 1 month ago)

Quote:

psykiidellic said:
could be a dead print




ive tried 3 different ones


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Invisiblenoobieshroomie
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Re: Germinating Spores [Re: rsinatra13]
    #11669645 - 12/17/09 04:20 PM (15 years, 1 month ago)

try to make a LC
and see if they germinate there

      -noobie-


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AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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Offlinersinatra13
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Re: Germinating Spores [Re: noobieshroomie]
    #11669690 - 12/17/09 04:26 PM (15 years, 1 month ago)

Quote:

noobieshroomie said:
try to make a LC
and see if they germinate there

      -noobie-




i have, they dont


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Invisiblenoobieshroomie
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Re: Germinating Spores [Re: rsinatra13]
    #11669704 - 12/17/09 04:28 PM (15 years, 1 month ago)

did you make LC with those 3 different prints?
did your spores come in direct contact with any chemicals
 
    -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

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Offlineinit
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Registered: 02/11/09
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Re: Germinating Spores [Re: rsinatra13]
    #11669713 - 12/17/09 04:29 PM (15 years, 1 month ago)

Do you think it's possible that the shot glass still had rubbing alcohol pooled at the bottom when you scraped your spores into it? That's the most plausible explanation I have, so thought I'd ask. I always worry about this myself since I use the same method to make syringes. (No problems here though.)


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Offlinersinatra13
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Re: Germinating Spores [Re: noobieshroomie]
    #11669718 - 12/17/09 04:30 PM (15 years, 1 month ago)

Quote:

noobieshroomie said:
did you make LC with those 3 different prints?
did your spores come in direct contact with any chemicals
 
    -noobie-




no only one...
and no they didnt

i guess i'll just keep trying. :thumbup:


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InvisibleCitizen13
Jack's wasted life.


Registered: 11/01/09
Posts: 1,319
Re: Germinating Spores [Re: rsinatra13]
    #11669745 - 12/17/09 04:32 PM (15 years, 1 month ago)

well the other day someone had the same problem and it turned out they were pressure cooking their syringes with the spores in them.

it took 4 pages of responses to figure that out, so in the interest of getting to the heart of the matter; are you doing that?

:lol:


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Offlineinit
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Re: Germinating Spores [Re: Citizen13]
    #11669758 - 12/17/09 04:34 PM (15 years, 1 month ago)

Quote:

Citizen13 said:
well the other day someone had the same problem and it turned out they were pressure cooking their syringes with the spores in them.

it took 4 pages of responses to figure that out, so in the interest of getting to the heart of the matter; are you doing that?

:lol:



Haha that was my first thought too.. but again I figured.. no way  :rofl:


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Invisiblenoobieshroomie
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Re: Germinating Spores [Re: init]
    #11669773 - 12/17/09 04:35 PM (15 years, 1 month ago)

yea that shit was funny:lmafo:

  -noobie-


--------------------
AMU

Best Thread Ever
CapZilla said:
not sure what GE and FAE are but i should probably get some.

Citric said:
Your signature is wrong on colonization temps!

GOOD JUDGMENT COMES FROM EXPERIENCE
EXPERIENCE COMES FROM BAD JUDGMENT

ROOM TEMP 70-75 IS BEST FOR COLONIZATION
Thank you mycochef

Extras: Filter Print Post Top
Offlinersinatra13
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Re: Germinating Spores [Re: Citizen13]
    #11669784 - 12/17/09 04:37 PM (15 years, 1 month ago)

Quote:

Citizen13 said:
well the other day someone had the same problem and it turned out they were pressure cooking their syringes with the spores in them.

it took 4 pages of responses to figure that out, so in the interest of getting to the heart of the matter; are you doing that?

:lol:




oh.. you pressure cook it before you put the spores in it?.... not after?...


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InvisibleCitizen13
Jack's wasted life.


Registered: 11/01/09
Posts: 1,319
Re: Germinating Spores [Re: rsinatra13]
    #11669817 - 12/17/09 04:39 PM (15 years, 1 month ago)

Quote:

rsinatra13 said:
Quote:

Citizen13 said:
well the other day someone had the same problem and it turned out they were pressure cooking their syringes with the spores in them.

it took 4 pages of responses to figure that out, so in the interest of getting to the heart of the matter; are you doing that?

:lol:




oh.. you pressure cook it before you put the spores in it?.... not after?...




sarcasm right?

or no?

but yeah, you never pressure cook spores.

:jefferson:


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Shop: Left Coast Kratom Buy Kratom Extract   Mushroom-Hut Liquid Cultures   OlympusMyco.com We’re Not Chasing Unicorns—We’re Building Quality (Olympus Myco Grow Bags)   North Spore Injection Grain Bag   Myyco.com Isolated Cubensis Liquid Culture For Sale


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